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EC number: 500-302-7 | CAS number: 113930-69-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 to 28 September 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This is a GLP Guideline study. All the validity criteria were met. Analytical test material characterization and quantification was included in the study report.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Because BADGE MXDA (#42) is a multi-component substance of limited solubility, WAFs were created and analyzed via non-purgable organic carbon (NPOC) and all values reported are nominal. This method is outlined in the OECD guidance (No. 23) on Aquatic Testing of Difficult Substances and Mixtures. NPOC was measured in test solutions at test initiation and termination.
- Vehicle:
- no
- Details on test solutions:
- A preliminary non-GLP trial indicated that a dosing (loading rate) of 100 mg BADGE-IPD (#42)/L required 24 hours of stirring to reach an equilibrium in water-soluble components (i.e., a change of <20% measured dissolved organic carbon concentrations between two sampling points); therefore, the WAF preparations for all test solutions were stirred for approximately 24 hours prior to test initiation. Each WAF preparation was made using a direct additon. Due to the low loading rates for the definitive study, 10 and 5 L of solution were made for the 0.43 and 0.94 mg/L loading rates, respecitvely; all other loading rates were made in 1–L volumes. Bulk test solutions were prepared using direct additions of 0.0043, 0.0047, 0.0021, 0.0045, and 0.0100 g into appropriate volumes of LDW to achieve loading rates of 0.43, 0.94, 2.1, 4.5, and 10 mg BADGE-MXDA (#42)/L. Aliquots were placed on glass cover slips and dropped directly into the appropriate volume of water in a glass vessel (glass battery jar for the 0.43 and 0.94 mg/L loading rates; 2-L glass beaker for all other loading rates). A stir bar was added and the solutions were coveredd and stirred for 24 hours prior to use. All solutions were clear and colorless.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organism was the freshwater cladoceran, Daphnia magna, from in-house cultures. Daphnid neonates less than 24 hours old from a laboratory-reared culture were used as the test organisms. Neonates were only used from daphnids which have had at least three broods. The day before neonates were needed for testing (<24 hours prior to test initiation), neonates within the culture vessels were removed leaving only adult daphnids in the vessels; these neonates were not used in the study. On the following day, neonates produced overnight in these same culture vessels were again removed from the vessels and placed into a glass beaker containing LDW. These neonates (<24 hours old) were held in an incubator until they were used for the study.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
- Hardness:
- 58 – 64 mg CaCO3/L
- Test temperature:
- 19 – 20°C
- pH:
- 7.4 – 7.7
- Dissolved oxygen:
- 8.4 – 8.9 mg/L (94 – 100% saturation)
- Nominal and measured concentrations:
- Nominal test concentrations: 0 (control), 0.43, 0.94, 2.1, 4.5, and 10 mg BADGE-MXDA (#42)/L
- Details on test conditions:
- Dissolved oxygen levels ranged from 8.4 – 8.9 mg/L (94 – 100% oxygen saturation), temperatures ranged from 19 – 20°C, and pH ranged from 7.4 – 7.7. Light intensity ranged from 740 – 939 lux. Alkalinity ranged from 38 – 40 mg CaCO3 at test initiation. Hardness ranged from 58 – 64 mg CaCO3 at test initiation. Conductivity ranged from 177 – 204 µmhos/cm at test initiation.
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 1.46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL 1.35-1.58 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 0.94 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobility
- Details on results:
- RESULTS (Based on Nominal Loading Rates): 24 hour EL50 = 1.71 mg/L (95% confidence interval = 1.47 – 1.99 mg/L); 48 hour EL50 = 1.46 mg/L (95% confidence interval = 1.35 – 1.58 mg/L); 48 hour NOELR = 0.94 mg/L (Based on highest loading rate exhibiting no statistically significant immobility or sublethal effects.)
- Reported statistics and error estimates:
- The 24-hour EL50 value, calculated by the Trimmed Spearman-Karber Program, was 1.71 mg/L, with 95% confidence intervals of 1.47 – 1.99 mg/L; the Spearman Karber trim was 0%. The 48-hour EL50 value, calculated by the Trimmed Spearman-Karber Program, was 1.46 mg/L, with 95% confidence intervals of 1.35 – 1.58 mg/L; the Spearman Karber trim was 0%.
• The 48-hour NOELR was 0.94 mg/L, calculated by the Fisher’s Exact Test. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 24-hour EL50 = 1.71 mg/L (95% confidence intervals 1.47 – 1.99 mg/L). The 48-hour EL50 = 1.46 mg/L ( 95% confidence intervals 1.35 – 1.58 mg/L). The 48-hour NOELR => 0.94 mg/L.
- Executive summary:
The purpose of this study was to evaluate the acute toxicity of BADGE-MXDA (#42) to the freshwater cladoceran, Daphnia magna, over a 48-hour exposure period. Based on the limited solubility of the test material, water accomodated fractions (WAFs) were used for exposures (OECD, 2000). The definitive test was conducted with four replicate vessels per test level, with five daphnids each, exposed to nominal loading rates of 0 (water control), 0.43, 0.94, 2.1, 4.5, and 10 mg BADGE-MXDA (#42)/L. The acute toxicity values for BADGE-MXDA (#42) with Daphnia magna were determined based on nominal loading rates. The results were as follows:
The 24-hour EL50 was 1.71 mg/L (95% confidence intervals 1.47 – 1.99 mg/L); the 48-hour EL50 was 1.46 mg/L ( 95% confidence intervals 1.35 – 1.58 mg/L). The 48-hour NOELR was => 0.94 mg/L.
Reference
None
Description of key information
One daphnia acute study
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.46 mg/L
Additional information
The purpose of this study was to evaluate the acute toxicity of BADGE-MXDA (#42) to the freshwater cladoceran, Daphnia magna, over a 48-hour exposure period. Based on the limited solubility of the test material, water accommodated fractions (WAFs) were used for exposures (OECD, 2000). The definitive test was conducted with four replicate vessels per test level, with five daphnids each, exposed to nominal loading rates of 0 (water control), 0.43, 0.94, 2.1, 4.5, and 10 mg BADGE-MXDA (#42)/L. The acute toxicity values for BADGE-MXDA (#42) with Daphnia magna were determined based on nominal loading rates. The results were as follows: The 24-hour EL50 = 1.71 mg/L (95% confidence intervals 1.47 – 1.99 mg/L); The 48-hour EL50 = 1.46 mg/L ( 95% confidence intervals 1.35 – 1.58 mg/L); The 48-hour NOELR = 0.94 mg/L
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