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EC number: 482-120-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Remarks:
- [C(97)186/Final]
Test material
- Details on test material:
- - Name of test material (as cited in study report): C-4000
- Physical state: liquid
- Analytical purity: 86.3 +/- 0.7%
- Purity test date: 2008-07-09
- Lot/batch No.: 51V034K7
- Expiration date of the lot/batch: 2008-10
- Storage condition of test material: refrigerated
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 10, 32, 100, 320, 1000 mg/L
- Sample storage conditions before analysis: refrigerated
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test material could not be easily weighed to
prepare test solutions on a weight to volume basis. Therefore, based on whole product, test
substance amounts were corrected for the density of C-4000 and prepared on a calculated weight
to volume basis. The test substance was added by direct volume to appropriately-labeled contact
flasks and contact flasks were transferred to the constant temperature chamber. Test substance
volumes of 4.95, 15.8, 49.5, 158, and 495 µL were added to the 10, 32, 100, 320, and 1,000 mg
whole product/L test flasks, respectively. A nominal 1,000 mg whole product/L abiotic control
was also prepared.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: The microbial inoculum used in the test was activated sludge collected August 27, 2008 from
Aeration Basin #1 at the Columbia Wastewater Treatment Plant in Columbia, Missouri.
Approximately 20 liters of activated sludge was collected.
- Preparation of inoculum for exposure: Twelve, 250 mL centrifuge bottles were filled with activated sludge and
concentrated by centrifugation at 3,000 rpm for approximately five minutes using IEC CR 6000 and Beckman Model J2-21 centrifuges. The supernatant was decanted, and the bottles were refilled with activated sludge and centrifuged three more times. After the final centrifugation, the supernatant was decanted, and the activated sludge was rinsed by adding ABC well water, shaking, and centrifuging as above. The supernatant was then decanted. The activated sludge pellet in each bottle was washed two more times in the same manner. The final supernatant was decanted, and a suspension of the sludge pellet was prepared by adding 200 mL of well water to each bottle. The microbial inoculum contained within the bottles were shaken then combined in a carboy, placed on a stir plate in an environmental chamber maintained at 20 ± 2 ºC, and aerated while stirring using compressed air. A synthetic sewage feed solution composed of peptone, beef extract, urea, NaCl, MgSO4·7H2O, CaCl2·2H2O, and K2HPO4 was used to sustain the inoculum overnight. The pH of the prepared activated sludge was 8.10.
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 1 d
- Remarks on exposure duration:
- Results from the range-finder indicated the respiration inhibition from the test substance was < 50% at the nominal loading rates; therefore a definitive test was not necessary.
Test conditions
- Hardness:
- 284 (2008-08-22)
286 (2008-08-28) - Test temperature:
- 19.9 (2008-08-22 )
20.3 (2008-08-28 ) - pH:
- 7.23 (2008-08-22 )
7.65 (2008-08-28 ) - Salinity:
- 634 (2008-08-22 )
640 (2008-08-28 ) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL glass flask
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 1000 mL
- Aeration: stirred and aerated using compressed air at 500 to 1000 mL/minute
- No. of organisms per vessel: 200 mL of microbial inoculum was added to each flask, except the abiotic control
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water (non-chlorinated) was used for all inoculum preparation procedures, synthetic sewage feed preparation, and test flask preparations; Reagent water was used to prepare the reference substance stock solution
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Light intensity: ambient light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption (respiration rate) and percent inhibition
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10, 32, 100, 320, and 1,000 mg whole product/L
- Results used to determine the conditions for the definitive study: Based on the results, a definitive test was not required and therefore not conducted. - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 1 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes; The percent inhibitions for the three reference substance flasks were 0, 9.4, and 65.3% at
3,5-dichlorophenol concentrations of 3.2, 10, and 32 mg/L, respectively. The estimated EC50 was 23.3 mg/L. This EC50 was within the 5 to 30 mg/L acceptability range as recommended by the OECD Guideline 209.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Since the percent inhibition at the highest concentration tested for C-4000 was less than 50%, the EC50 was reported as >1,000 mg whole product/L. The respiration rates of control flasks as well as the estimated EC50 values of the reference toxicant testing met the acceptability criteria outlined in OECD Guideline 209.
- Executive summary:
A test was conducted to determine the effects of C-4000 on microorganisms by measuring the respiration rate under defined conditions in the presence of varying concentrations of the test substance. The microbial inoculum was exposed to nominal concentrations of 0 (control), 10, 32, 100, 320, and 1,000 mg whole product/L.
The results of this test showed that the respiration rates of the two control flasks were within 15% of the mean respiration rate which satisfies the criteria for acceptability outlined in the test guideline concerning data from the control flasks.
The test substance showed 0, 0, 0, 18.1, and 0% inhibition at test concentrations of 10, 32, 100,
320, and 1,000 mg whole product/L. Under the conditions of the study, the estimated EC50 for
C-4000 was >1,000 mg whole product/L based on the nominal loading rate. Based on these
results, a definitive test was not required and therefore not conducted.
The three reference substance flasks exhibited percent inhibition values of 0.0, 9.4, and 65.3% at
3,5-dichlorophenol concentrations of 3.2, 10, and 32 mg/L, respectively. An EC50 value was
calculated to be 23.3 mg/L for 3,5-dichlorophenol, which was within the acceptable value range
of 5 – 30 mg/L for the reference substance.
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