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EC number: 429-220-9 | CAS number: 132584-17-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 429-220-9
- EC Name:
- -
- Cas Number:
- 132584-17-9
- Molecular formula:
- C17 H18 O4
- IUPAC Name:
- ethyl 2-hydroxy-2-(4-phenoxyphenyl)propanoate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Purity: 73.5%
Batch: IN-JG303-7
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC
- Details on sampling:
- Concentrations of IN-JG303 for each of the test, solvent control, and blank control solutions were verified for the 0-hour (day 0) samples
Concentrations of IN-JG303 for each of the test, solvent control, blank control, and abiotic
control (nominal 10 mg/L) solutions were verified for the 72-hour (day 3) samples. Samples from each of the test, solvent control, blank control, and abiotic control solutions also
were collected at test termination. These samples were prepared by pooling all three replicates
for each of the test and control solutions. Aliquots were taken from each of the pooled samples and also given to the analytical chemist for analysis.
All samples were analyzed on day of receipt.
Test solutions
- Vehicle:
- yes
- Remarks:
- DMF
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: DMF at a maximum 0.1 ml/l
Test organisms
- Test organisms (species):
- Selenastrum sp.
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: Selenastrum capricornutum
- Source (laboratory, culture collection): The original culture source was supplied by Michael Ziegenfuss, The Academy of Natural Sciences of Philadelphia, 1900 Benjamin Franklin Parkway, Philadelphia, PA, 19103-1195
ACCLIMATION
Prior to the study, Selenastrum capricornutum cultures were maintained under the same environmental conditions used in the study. The organisms were cultured in sterilized 250 mL Erlenmeyer flasks containing approximately 50 mL of filtered (= filter-sterilized) AAP nutrient medium and were aseptically transferred to fresh medium every 3 to 7 days. The flasks were fitted with foam stoppers to permit gas exchange.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24.7 to 24.8°C
- pH:
- 7.28 to 7.73 at the 0-hour and from 7.44 to 7.89 at the 72-hour interval
- Nominal and measured concentrations:
- Nominal: 0, 0.63, 1.25, 2.5, 5, and 10 mg/L
Measured: 0, 0.53, 0.97, 1.9, 3.9, and 7.8 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: sterilized 250 mL Erlenmeyer flasks each fitted with sterilized foam stoppers
- Initial cells density:
- Control end cells density: To achieve the desired nominal concentration of approximately 10,000 Selenastrum capricornutum cells/mL at test initiation, a volume (approximately 0.162 mL) of algal inoculum from a logarithmically growing stock culture was aseptically transferred to each, excluding the
abiotic control, flask (test unit).
GROWTH MEDIUM
Synthetic algal-assay-procedure (AAP) nutrient medium(3-4) was prepared by adding one mL of each of the six macronutrient stock solutions and one mL of the micronutrient stock solution to approximately 800 mL of Milli-Q® water (deionized), mixing after each addition. The volume of the medium was brought to one liter with additional Milli-Q® water. Since larger volumes of the medium were required for the definitive and recovery tests, appropriate proportions (one mL of each stock solution for each one liter of medium) were used. The medium pH was adjusted to approximately 7.5 with 0.1 N hydrochloric acid. For both the
definitive and recovery tests, the medium was filter-sterilized using Corning® pre-sterilized filtration systems with 0.22 μm cellulose acetate filters. The containers with the resulting filtersterilized AAP nutrient medium for use in the definitive and recovery tests were stored in the refrigerator in the dark at approximately 4°C and acclimated to room temperature prior to use. Any remaining unused medium was properly stored.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24 hours
- Light intensity and quality:6,000 to 10,000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
-The mean healthy cell count, area under the growth curve, and growth rate at the 72-hour interval for each test concentration were expressed relative to the pooled blank and solvent controls.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.2 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.63 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.63 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 72-hr EC50 (healthy cell count) = 1.5 mg/L (95% C.I. = 1.4 to 1.6 mg/L)
72-hr NOEC (healthy cell count) = 0.63 mg/L
72-hr EC50 (area under the curve) = 1.6 mg/L (95% C.I. = 1.4 to 1.7 mg/L)
72-hr NOEC (area under the curve) = 0.63 mg/L
72-hr EC50 (growth rate) = 5.2 mg/L (95% C.I. = 4.9 to 5.6 mg/L)
72-hr NOEC (growth rate) = 0.63 mg/L
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