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EC number: 266-235-8 | CAS number: 66204-44-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study according to guideline (OECD 209).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (dated 04.04.1984)
- Deviations:
- yes
- Remarks:
- ph value is not reported
- GLP compliance:
- no
Test material
- Reference substance name:
- 3,3'-methylenebis[5-methyloxazolidine]
- EC Number:
- 266-235-8
- EC Name:
- 3,3'-methylenebis[5-methyloxazolidine]
- Cas Number:
- 66204-44-2
- Molecular formula:
- C9H18N2O2
- IUPAC Name:
- 3,3'-methylenebis[5-methyloxazolidine]
- Details on test material:
- - Test material: Reaction products of paraformaldehyde and 2-hydroxypropylamine (ratio of 3:2)
- Charge No. 620251
- Specification: MAR 71
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Nature: Activated sludge
- Source: sewage treatment plant
- Sampling site: Stellinger Moor, Hamburg (Germany)
- Laboratory culture: No
- Method of cultivation: Not applicable
- Preparation of inoculum for exposure: Sludge diluted with 5% (v/v) synthetic waste water, aerated overnight and used without further pretreatment
- Pretreatment: None
Study design
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 ± 1°C
- Nominal and measured concentrations:
- Nominal concentrations: 10, 20, 50, 100, 300 mg/L + control
- Details on test conditions:
- CULTURE MEDIUM
- 8 mL synthetic sewage were diluted to 150 mL with tap water, then 100 mL microbial inoculum were added
TEST SYSTEM
- Number of culture flasks/concentration: 1
- Aeration device: Membrane pump
- Measuring equipment: O2-electrode
- Test performed in closed vessels due to significant volatility of TS: No
OTHER TEST CONDITIONS
- Suspended solids concentration: 3.09 g/L filtratable solids
TEST PARAMETER
- Respiration rate (Measurement of dissolved oxygen concentration)
- After 3 h respiration rate measured over 10 min with oxygen electrode
CONTROLS
- 2 blank controls (without test substance), each 1 at the beginning and at the end of test
STATISTICS
- EC50 taken from concentration-response curve - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 44 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - No other effects observed
- Results with reference substance (positive control):
- - Concentrations: 10, 20, 30, 40, 50 mg/L + control (nominal concentrations)
- Results: 3 h-EC50 = 9 mg/L - Reported statistics and error estimates:
- Deviation of respiration in controls ± 2%
Any other information on results incl. tables
Concentration (mg/L) |
Respiration rate (mg O2/L*min) |
Inhibition (%) |
Control |
0.87 |
-2 |
10 |
0.76 |
10 |
20 |
0.74 |
13 |
50 |
0.36 |
58 |
100 |
0.11 |
87 |
300 |
0.03 |
96 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is harmful towards bacteria.
- Executive summary:
The substance was found to be toxic towards sewage sludge microorganisms.
A clear concentration-response relationship can be established from the study results. At 10 mg/L respiration was inhibited by 10%, compared to the control. At 50 mg/L respiration was inhibited by 58%, while at the highest applied concentration inhibition was nearly total.The EC50 was determined at 44 mg/L.
The test is considered as valid as the validity criteria given in OECD 209 (as adopted 1981) were met: a) the difference of the duplicate controls was within a range of 15%; b) the sensitivity of the activated sludge towards the reference substance was within the accepted range (EC50 between 5 and 30 mg/L).
The constituents of the reaction product (substance) are not volatile from aqueous solutions, therefore this removal mechanism could not influence the test results. It is not expected that the the low adsorptivity of the test substance influenced test substance concentrations.
The constituents of the reaction product (substance) hydrolyse completely in concentrations which are expected to occur in waste waters and surface waters. Also in the media of toxicity tests, complete hydrolysis is expected, Therefore, the observed effects are assumed to be caused by a mixture of hydrolysis products.
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