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EC number: 249-894-6 | CAS number: 29857-13-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: originated from the sewage treatment plant from Hildesheim
- Preparation of inoculum for exposure: after sedimentation washed twice with declorinated and sterilized tap water
- Initial biomass concentration: the biomass was 3.32 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18-22-°C
- Reference substance (positive control):
- yes
- Remarks:
- Copper (II)sulfate-pentahydrate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 84.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 130 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 650 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: not reported - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50 = 120 mg/L, need to be within 96.9 to 135.3 mg/L., hence the refrence substance indicated that the test system is valid - Reported statistics and error estimates:
- Statistics performed according to "Ausgewählte Methoden der Wasseruntersuchung", Band II, VEB Fischer Verlag
- Validity criteria fulfilled:
- yes
- Executive summary:
In the Klimisch 1 study from Noack (1991) the respiration inhibition of Netzer SB 10 (which contains 65% a.i.) to activated sludge was determined in a 3 h static GLP respiration inhibition test according to OECD 209. The nominal test concentrations were 0 (control), 32, 100, 180, 320, 580 and 1000mg Netzer SB 10/L, corresponding to 0 (control), 20.8, 65, 117, 208, 377 and 650 mg a.i./L. Two control replicates were set up. Dose verification analysis was not performed. The respiration was measured using an oxygen probe. The EC20, EC50 and EC80 was calculate by regression of the log transformed concentration vs. probit transformed percent inhibition relative to the average control respiration. The EC20 and EC50 were 130 and > 1000 mg Netzer SB 10 /L, respectively. These values correspond to EC20 and EC50 values of 84.5 and > 650 mg a.i./L.
This study is considered to be acceptable for the risk assessment.
Reference
Table 1. Repiration rates and inhibition in the test and reference substance
Substance |
Concentration [mg test substance/L] |
Respiration rate [mg O2/h/h] |
Inhibition [%] |
Control 1 |
- |
34.8 |
- |
Control 2 |
- |
36.4 |
- |
Test substance |
32 |
32.0 |
10 |
|
58 |
31.6 |
11 |
|
100 |
28.8 |
19 |
|
180 |
25.6 |
28 |
|
320 |
23.6 |
34 |
|
580 |
24.0 |
33 |
|
1000 |
20.4 |
43 |
Reference substance |
58 |
31.6 |
11 |
|
100 |
20.8 |
42 |
|
180 |
6.4 |
82 |
Description of key information
OECD 209: 3 h EC50: > 650 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 650 mg/L
Additional information
In the Klimisch 2 study from Noack (1991) the respiration inhibition of Netzer SB 10 (which contains 65% a.i.) to activated sludge was determined in a 3 h static GLP respiration inhibition test according to OECD 209. The nominal test concentrations were 0 (control), 32, 100, 180, 320, 580 and 1000mg Netzer SB 10/L, corresponding to 0 (control), 20.8, 65, 117, 208, 377 and 650 mg a.i./L. Two control replicates were set up. Dose verification analysis was not performed. The respiration was measured using an oxygen probe. The EC20, EC50 and EC80 was calculate by regression of the log transformed concentration vs. probit transformed percent inhibition relative to the average control respiration. The EC20 and EC50 were 130 and > 1000 mg Netzer SB 10 /L, respectively. These values correspond to EC20 and EC50 values of 84.5 and > 650 mg a.i./L.
This study is considered to be acceptable for the risk assessment.
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