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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
long-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(E)-anethole
EC Number:
224-052-0
EC Name:
(E)-anethole
Cas Number:
4180-23-8
Molecular formula:
C10H12O
IUPAC Name:
1-methoxy-4-prop-1-en-1-ylbenzene

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
flow-through
Water media type:
other: tap water
Limit test:
yes
Total exposure duration:
28 d

Test conditions

Test temperature:
26  1.5 °C
pH:
7
Nominal and measured concentrations:
0.2 / 1 / 5 mg/L
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
ca. 0.34 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
ca. 2.18 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
ca. 4.14 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
At the highest concentration 5 mg/L, only a few larvae hatched. A few days later, all larvea were dead in all replicates of this concentration.
Therefore, no further analytical measurement was performed at this concentration.
A clear toxicity was also observed at a concentration of 1 mg/L. Only a few surviving larvae are still present at this time.
At the beginning and on day 7 samples were taken from the aquarium. On day 7 the measured concentrations were much too low.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
E-anethole is classified as Long-term (chronic) aquatic hazard Category Chronic 3: