Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 212-849-6 | CAS number: 873-69-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-05-23 - 2017-06-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- Version adopted 23. Mar. 2006, Annex 5 corrected: 28 July 2011
- Deviations:
- yes
- Remarks:
- Deviations from the Study Plan, see "Any other information on material and methods incl. tables", but none from the guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Version adopted 07. December 2015
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pyridine-2-carbaldehyde oxime
- EC Number:
- 212-849-6
- EC Name:
- Pyridine-2-carbaldehyde oxime
- Cas Number:
- 873-69-8
- Molecular formula:
- C6H6N2O
- IUPAC Name:
- pyridine-2-carbaldehyde oxime
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored in a tightly closed vessel at room temperature (20 ± 5 °C).
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 1.0, 3.2, 10, 32 and 100 mg/L
- Sampling method: not specified. Samples for the analytical determination were taken at test start and test end.
- Sample storage conditions before analysis: not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution containing the load of 100.0 mg/L in algal medium (demineralised water enriched with minerals but without algae) was prepared. The lower treatments were prepared by dilution of this stock solution with nutrient medium.
- Controls: medium without any test item
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): none stated
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus (unicellular freshwater green alga)
- Strain: Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen [SAG], Strain-No. 86.81
- Source (laboratory, culture collection): The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen)
- Age of inoculum (at test initiation): 4 days
- Method of cultivation: The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
ACCLIMATION
- Acclimation period: 4 days
- Culturing media and conditions (same as test or not): An aliquot of the permanent culture was brought into nutrient medium and incubated under continuous lighting for 96 hours. The resulting culture grew exponentially.
Before usage, the pre-culture was checked for the absence of cell aggregates and the cell number of culture was determined.
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.0 - 23.1 °C
- pH:
- 7.7 at test start
7.6 - 7.8 at test end - Nominal and measured concentrations:
- Nominal: 0, 1.0, 3.2, 10, 32 and 100 mg/L
Found (t0):Found (t72h): LOQ (Limit of quantification) = 0.2 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks
- Type (delete if not applicable): open (covered with perforated plastic foil)
- Material, size, headspace, fill volume: glass flasks with a total volume of 65 mL, filled with 45 ± 1 mL of the respective test solution
- Aeration: no
- Initial cells density: 3.9 *103 cells/mL
- Control end cells density: 149640 cells/mL (mean) +/- 11906 cells/mL (standard deviation)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Stock Solution I:
NH4Cl 1500 mg
MgCl2*6H2O 1200 mg
CaCl2*2H2O 1800 mg
MgSO4*7H2O 1500 mg
KH2PO4 160 mg
H2O deionised ad 1000 mL
Stock Solution II:
FeCl3*6H2O 64 mg
Na2EDTA*2H2O 100 mg
H2O deionised ad 1000 mL
Stock Solution III:
H3BO3 185 mg
MnCl2*4H2O 415 mg
ZnCl2 3 mg
CoCl2*6H2O 1.5 mg
CuCl2*2H2O 0.01 mg
Na2MoO4*2H2O 7 mg
H2O deionised ad 1000 mL
Stock Solution IV:
NaHCO3 50 g
H2O deionised ad 1000 mL
Algal Medium:
Stock Solution I 10.0 mL
Stock Solution II 1.0 mL
Stock Solution III: 1.0 mL
Stock Solution IV 1.0 mL
H2O deionised ad 1000 mL
Stock solutions I and III and the used demineralised water were sterilized using an autoclave. Stock solutions II and IV were sterilized using filtration. The algal medium corresponds to the OECD TG 201 medium.
Deviations from the nominal weighted loads were less than 5%.
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous
- Light intensity and quality: 7200 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: The cell number was determined with an electronic particle counter before the start of incubation and every 24 hours.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.125
- Test concentrations: 0, 1.0, 3.2, 10, 32 and 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a separate reference test.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 48 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 8.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): normal and healthy appearance of the algae at the end of the test
- Unusual cell shape: no
- Colour differences: no
- Flocculation: not stated
- Adherence to test vessels: not stated
- Aggregation of algal cells: not stated
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
The 72h-EC50s of potassium dichromate were determined in a separate reference test. The values were within the range of the laboratory. The results of the last study with the positive control K2Cr2O7 were:
72h ErC50 = 0.94 mg/L (0.90 - 0.98 mg/L)
72h EyC50 = 0.42 mg/L (0.39 - 0.45 mg/L)
The study was performed under GLP conditions in February 2017. - Reported statistics and error estimates:
- Calculation of results was performed with the help of validated software (Microsoft Excel®). The estimation of the biological data was accomplished using the software ToxRat® Professional, version 3.2.1. Biological results are stated using two significant digits.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was performed according to OECD TG 201 with no relevant deviations (only minor deviation from the study plan with no influence on the integrity of the study, see "Any other information on materials and methods incl. tables"), the results were so obtained via a scientifically reasonable method. The validity criteria for the study as given by the OECD Guideline 201 were met and the study is therefore considered to be valid. Hence, there is no doubt that the obtained results are not reliable: The toxic effect was investigated by determination of the inhibition of the growth rate of the algae and the yield during the exposure period of 72 hours. The maximum concentration investigated was 100 mg/L (nominal).
At the start and at the end of the test, the content of the test item in the test solutions with and without algae was determined using HPLC.
The measured concentrations lay between 89 % and 99 % of the nominal concentrations at the beginning of the test and between 98 % and 111 % of the nominal concentrations at the end of the test. As the measured concentrations were within ± 20 % of the nominal concentrations, the determination of the results was based on nominal concentrations.
The following EC-values (72h; growth rate; nominal) were determined accordingly:
LOEC (growth rate and yield) = 3.2 mg/L
NOEC (growth rate and yield) = 1.0 mg/L
EC50 (growth rate) = 48 mg/L
Based on these results, the test item does not need to be classified as acute toxic to the aquatic environment. With regard to chronic toxicity, taking into account the facts that the test item is soluble in water, i.e. above the determined EC50 value, and not readily biodegradable, the test item should be classified as aquatic chronic Cat. 3 according to the Regulation (EC) No. 1272/2008. - Executive summary:
The study was performed according to OECD TG 201 under GLP using 5 concentrations ranging from 1 to 100 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations: 3.2 / 10 / 32 / 100 mg/L nominal concentration
At the start and at the end of the test, the content of the test item in the test solutions with and without algae was determined using HPLC. The measured concentrations lay between 89 % and 99 % of the nominal concentrations at the beginning of the test and between 98 % and 111 % of the nominal concentrations at the end of the test. As the measured concentrations were within ± 20 % of the nominal concentrations, the determination of the results was based on nominal concentrations.
The 72h-EC50s of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 - 0.66 mg/L).
The following results for the test item pyridine-2-carbaldehyde oxime were determined:
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
1.0 mg/L
3.2 mg/L
12 mg/L
48 mg/L
Yield
1.0 mg/L
3.2 mg/L
8.2 mg/L
20 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.