2,4-di-tert-pentylphenol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2.4-di-tert.-Amylphenol

IUPAC Name:

2.4-di-tert.-Amylphenol

Details on test material:

- Name of test material (as cited in study report): 2,4-di-tert-amylphenol
- Physical state: liquid
- Analytical purity: 99.5
- Sample No.: 1754/991007
- Date of manufacture: 1999-03-03

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus, clone 5
- Source: laboratory breed
- Age at study initiation: not older than 24 hours
- Method of breeding: The females were maintained in M4 medium in 1 l beakers. A reddish dark-brown coloration of the animals indicated that they were healthy. At two to three day intervals, the exuviae were siphoned off and the water was changed. In the course of this, the offspring was removed from the breeding vessels. At regular intervals of approx. 4 weeks, offspring was isolated from the vessels. They served as a basis for further breeding.
- Feeding during test: none

ACCLIMATION
- Acclimation period: Females were seperated from the offspring 24 hours before the start of the test. The offspring produced overnight were transferred into test water and were used for the test.
- Type and amount of food: Scenedesmus subspicatus, the amount of food added is such that it is consumed by the animals
- Feeding frequency: daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

2.5 mmol

Test temperature:

20 °C +/- 1 °C

pH:

7.7-8.1

Dissolved oxygen:

86 - 105% O2 saturation

Salinity:

CaCl2 x 2 H2O: 294 mg/L; MgSO4 x 7 H2O: 114 mg/L; NaHCO3: 65 mg/L; KCl: 6 mg/L
Ca/Mg ratio: 4:1
Na/K ratio: 10:1

Nominal and measured concentrations:

Nominal: 0.057, 0.11, 0.23, 0.46, 0.91, 1.80, 3.60 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Round-bottom test tubes
- Material, size, headspace, fill volume: glass, calibrated to 10 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 114 mg/l; NaHCO3: 65 mg/l; KCl: 6 mg/l)
- Ca/Mg ratio: 4:1
- Conductivity: not mentioned
- Culture medium different from test medium: yes
- Intervals of water quality measurement: not mentioned

OTHER TEST CONDITIONS
- Adjustment of pH: not adjusted
- Photoperiod: not applicable
- Light intensity: no light during test

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation after 24 and 48 hours; O2 concentration, pH at the end of the test

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned
- Range finding study: not mentioned

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

1.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 1.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

ca. 0.91 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

ca. 1.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Results with reference substance (positive control):

- Results with reference substance valid? yes, historical control (not tested in parallel to the test substance)
- Concentrations: 1.0 and 2.0 mg/L potassium dichromate
- Results: 45% at 1.0 mg/L, resp. 100% at 2.0 mg/L immobilised daphnia after 24 h

Reported statistics and error estimates:

no statistics performed, the EC50 values were calculated graphically (dose-response relationship).

Any other information on results incl. tables

Table: Effect of 2,4 -di-tert. Amylphenol on immobilisation of Daphnia magna

concentration [mg/L]	24 hour values			48 hour values
	number		%	number		%
	mobile	immobile	immobile	mobile	immobile	immobile
0 (control)	20	0	0	20	0	0
0.057	20	0	0	20	0	0
0.11	20	0	0	20	0	0
0.23	20	0	0	20	0	0
0.46	20	0	0	19	1	5
0.91	20	0	0	18	2	10
1.80	6	14	70	0	20	100
3.60	3	17	85	0	20	100