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Diss Factsheets
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EC number: 200-143-0 | CAS number: 52-51-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted in accordance with GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- - Stock solutions were prepared immediately before use (ca. 30 min before dosing the activated sludge) by making up 1 g of Bronopol to 1 litre with water.
- Tap water was used as the diluent.
- The dilution water used was aerated tap water held at ca. 21 +/- 2°C for at least 24 hours before use. - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Inoculum: activated sludge freshly collected on day of test from Beford Sewage Treatment Works, Meadow Lane, Bedford
- Treatment: sludge was centrifuged and washed with aerated tap water three times before use. The pellet was re-suspended in tap water to give a dry weight level of 4 g/L in the final inoculum. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 150 min
- Test temperature:
- 20 - 23 °C
- pH:
- 7.75 - 8.40
- Nominal and measured concentrations:
- - Concentrations tested (nominal): 0, 2, 6.3, 20, 63 and 200 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessels: glass BOD bottles with ground glass stoppers (300 ml volume nominal)
- Concentration of inoculum: 10 ml of the final inoculum were added to each test vessel giving a final concentration of 0.14 g/L dry weight, which was equivalent to 2.3 g/L wet weight in each test vessel - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 150 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 150 min
- Dose descriptor:
- EC50
- Effect conc.:
- 43 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- 150 min EC50 = 43 mg/L (nominal)
150 min EC80 = 887 mg/L (nominal) - Results with reference substance (positive control):
- EC50 = 12 mg/L
Reference
Description of key information
Depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 43 mg/L
Additional information
The effects of bronopol to aquatic microorganisms were investigated in a GLP guideline study according to OECD 209 with activated sludge [Knoll MicroCheck 1996]. At test termination after 2.5 h an EC20 of 2 mg/L (nominal) was determined.
Additionally, another GLP guideline study according to OECD 209 with activated sludge as inoculum was conducted [BASF SE 2002]. After the test duration of 30 min an EC20 of ca. 20 mg/L (nominal) was determined and inhibition of respiration in activated sludge ranged from 14 to 67 %. In a third study, the effect of Bronopol on the growth of Pseudomonas putida was examined according to ISO guideline 10712 [Knoll MicroCheck 1996]. After 16 h of incubation an EC10 of 0.5 mg/L (nominal) was determined.
The effect concentrations obtained from the latter two studies were comparable to the ones retrieved from the key study and can therefore be used as supporting evidence of the acute toxicity of bronopol to aquatic microorganisms.
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