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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12.05.2000 to 15.05.2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to the appropriate OECD test guideline, and in compliance with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dequest 2066A
- Substance type: Phosphonate
- Physical state: Clear amber liquid
- Stability under test conditions: Not determined
- Storage condition of test material: Room temperature
No further details available.

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: Myrtle's Rabbitry, Thompson Station, TN.
- Age at study initiation: 'adult'
- Weight at study initiation: 2.5 - 2.9 kg
- Housing: Individually in suspended stainless steel cages.
- Diet (e.g. ad libitum): Ad libitum
- Water (e.g. ad libitum): Ad libitum
- Acclimation period: Minimum of five days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18-22
- Humidity (%): 46-82
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: 12.05.2000 to 15.05.2000

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 ml
Observation period (in vivo):
72 hours
Number of animals or in vitro replicates:
Six
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): No

SCORING SYSTEM: EEC Ocular Evaluation Criteria

TOOL USED TO ASSESS SCORE: auxiliary light source, fluorescein

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 24, 48 and 72 hour
Score:
4.11
Reversibility:
fully reversible
Remarks on result:
other: considered non-irritating
Irritant / corrosive response data:
Exposure to the test substance produced corneal opacity in 4/6 test eyes at the 1-hour scoring interval. The corneal opacity resolved in all test eyes by the 24hour scoring interval. Iritis was observed in 6/6 test eyes at the 1-hour scoring interval and resolved completely in all test eyes by the 48-hour scoring interval. Conjunctivitis (redness, swelling and/or discharge) was noted in 6/6 test eyes at the 1-hour scoring interval. The conjunctival irritation resolved completely in all test eyes by the 72-hour scoring interval. Additional ocular findings included slight dulling of the normal luster of the cornea which was noted in 2/6 test eyes.
A mechanical abrasion was observed in 1/6 control eyes during the fluorescein examination, but was not considered to be significant due to its transient nature. No corneal opacity, iritis or conjunctivitis was observed in the control eyes.
Other effects:
None reported.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information
Conclusions:
In an eye irritation study conducted to OECD 405 and GLP (reliability score 1) Dequest 2066A (sodium salt of DTPMP) was not irritating to the eyes of rabbits.