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Diss Factsheets

Ecotoxicological information

Toxicity to terrestrial arthropods

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Endpoint:
toxicity to terrestrial arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
GLP compliance:
not specified
Application method:
soil
Specific details on test material used for the study:
Na2HAsO4.7H2O
Analytical monitoring:
yes
Details on sampling:
100 mg aliquot of dry soil sub-sample digested in a 3:1:1 (v/v/v) mixture of HF, HClO4 and HNO3 at 180°C for 45 min in a closed microwave digestion device
Vehicle:
no
Details on preparation and application of test substrate:
spraying As aqueous solution to a thin layer of soil and thoroughly mixed
Test organisms (species):
Folsomia candida
Animal group:
Collembola (soil-dwelling springtail)
Study type:
laboratory study
Limit test:
no
Total exposure duration:
28 d
Remarks:
15 or 150 days aging after spiking prior to testing
Test temperature:
20 °C
pH (if soil or dung study):
JX red soil: 4.91
GD red soil: 4.94
HUN paddy soil: 6.6
AH yellow-brown soil: 5.29
HLJ black soil: 5.78
YN yellowish-red soil: 6.93
CQ purple soil: 6.4
SX loessal soil: 8.25
HN fluvoaquic soil: 8.35
XJ gray desert soil: 8.17
Humidity:
55 - 60% of WHC
Photoperiod and lighting:
16h light/8h dark regime
Details on test conditions:
Ten 10–12 d old juveniles were transferred to each test jar. Four replicates of each As concentration treatment were performed. The Folsomia candida in each jar were fed a few grains of dried yeast once each week. The moisture content of the soil in each jar was checked by weighing the jar, and deionized water was added as necessary to maintain the desired moisture content. This process also involved aerating the soil in each jar.
Nominal and measured concentrations:
For soils with pH > 8: nominal concentrations of 0, 10, 20, 40, 80, and 160 mg As/kg soil dry weight
For other soils: nominal concentrations of 0, 40, 80, 160, 320, and 640 mg As/kg soil dry weight
The measured total As concentrations found in the soil samples agreed well with the nominal concentrations.
Reference substance (positive control):
no
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
320 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: JX red soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
640 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: JX red soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
580 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: JX red soil
Remarks:
150 d aging
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
320 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: GD red soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
640 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: GD red soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
531 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: GD red soil
Remarks:
150 d aging
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
160 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HUN paddy soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
320 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HUN paddy soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
487 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HUN paddy soil
Remarks:
150 d aging
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
80 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: AH yellow-brown soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
160 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: AH yellow-brown soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
366 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: AH yellow-brown soil
Remarks:
150 d aging
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
160 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HLJ black soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
320 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HLJ black soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
304 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HLJ black soil
Remarks:
150 d aging
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
80 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: YN yellowish-red soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
160 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: YN yellowish-red soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
195 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: YN yellowish-red soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
<= 40 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: CQ purple soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
113 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: CQ purple soil
Remarks:
150 d aging
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
40 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: SX loessal soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
80 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: SX loessal soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
95 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As(total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: SX loessal soil
Remarks:
150 d aging
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
40 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HN fluvoaquic soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
80 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HN fluvoaquic soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
83 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HN fluvoaquic soil
Remarks:
150 d aging
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
20 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: XJ gray desert soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
40 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: XJ gray desert soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
67 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: XJ gray desert soil
Remarks:
150 d aging
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
320 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: JX red soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
640 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: JX red soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
284 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: JX red soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
160 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HLJ black soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
320 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HLJ black soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
187 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: HLJ black soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
<= 40 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: CQ purple soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
74 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: CQ purple soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
20 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: XJ gray desert soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
40 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: XJ gray desert soil
Remarks:
15 d aging
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
50 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
element
Remarks:
As (total concentration)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: XJ gray desert soil
Remarks:
15 d aging
Results with reference substance (positive control):
not available
Reported statistics and error estimates:
EC50 calculated by the logistic sigmoidal nonlinear model
NOEC & LOEC: significant differences derived with one way analysis of variance (ANOVA) (p < 0.05)
Validity criteria fulfilled:
yes
Conclusions:
Nine reliable 28-d NOEC values for soils aged for 150 days after spiking ranged between 20 and 320 mg As/kg soil (based on added As concentration in soil).
The 28-d EC50 for the toxicity of As to the reproduction of springtail in ten soils aged for 150 d ranged between 67 and 580 mg As/kg soil dry weight.
Compared to soils aged for only 15 d, these concentrations inhibiting 50% reproduction were 1.3- to 2.0-fold higher.
Endpoint:
toxicity to terrestrial arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
Version / remarks:
Folsomia candida
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 232 (Collembolan Reproduction Test in Soil)
Version / remarks:
Folsomia candida
Deviations:
no
GLP compliance:
no
Application method:
soil
Specific details on test material used for the study:
Na2HAsO4.7H2O
Analytical monitoring:
yes
Remarks:
background concentration and confirmation of nominal concentrations
Vehicle:
no
Details on preparation and application of test substrate:
Test substance added as aqueous solution and mixed with soil
Test organisms (species):
Folsomia candida
Animal group:
Collembola (soil-dwelling springtail)
Study type:
laboratory study
Limit test:
no
Total exposure duration:
28 d
Remarks:
7 days equilibration after spiking prior to testing
Test temperature:
20
pH (if soil or dung study):
LUFA 2.2 - Dystric Cambisol: 5.6
Refesol 05-G: 5.2
Refesol 06-A: 7.3
Ter Munck - Haplic Luvisol: 5.7
Woburn - Dystric Cambisol: 6.0
Zegveld - Histosol: 4.8
Humidity:
LUFA 2.2 - Dystric Cambisol: 50% of WHC
Refesol 05-G: 40% of WHC
Refesol 06-A: 45% of WHC
Ter Munck - Haplic Luvisol: 50% of WHC
Woburn - Dystric Cambisol: 45% of WHC
Zegveld - Histosol: 50% of WHC
Photoperiod and lighting:
16 h day with a light intensity of 400 - 800 lux
8 h night
Details on test conditions:
At the beginning of the test (day 0) 8 – 10 mg dry yeast was added to each test vessel.
The vessels were covered with perforated Parafilm to allow air exchange and then weighted.
Weight losses were documented on a weekly basis (day 7, 14, and 21) and compensated if necessary.
At day 14, test animals were fed again with 8 – 10 mg dry yeast.
Nominal and measured concentrations:
Nominal:
LUFA 2.2 - Dystric Cambisol: 0, 5.6, 10, 17.8, 31.6, 56.2, and 100 mg As/kg soil dry weight
Refesol 05-G: 0, 100, 178, 316, 562, and 1000 mg As/kg soil dry weight
Refesol 06-A: 0, 100, 178, 316, 562, and 1000 mg As/kg soil dry weight
Ter Munck - Haplic Luvisol: 0, 10, 17.8, 31.6, 56.2, and 100 mg As/kg soil dry weight
Woburn - Dystric Cambisol: 0, 100, 178, 316, 562, and 1000 mg As/kg soil dry weight
Zegveld - Histosol: 0, 100, 178, 316, 562, and 1000 mg As/kg soil dry weight

Measured As concentrations are within 20% of nominal values.
Reference substance (positive control):
no
Key result
Duration:
28 d
Dose descriptor:
EC10
Effect conc.:
32.2 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: LUFA 2.2
Remarks:
Dystric Cambisol
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
31.6 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: LUFA 2.2
Remarks:
Dystric Cambisol
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
56.2 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: LUFA 2.2
Remarks:
Dystric Cambisol
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
51 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: LUFA 2.2
Remarks:
Dystric Cambisol
Key result
Duration:
28 d
Dose descriptor:
EC10
Effect conc.:
129.4 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Refesol 05-G
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
178 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Refesol 05-G
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
316 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Refesol 05-G
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
285 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Refesol 05-G
Key result
Duration:
28 d
Dose descriptor:
EC10
Effect conc.:
147.4 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Refesol 06-A
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
178 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Refesol 06-A
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
316 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Refesol 06-A
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
344.3 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Refesol 06-A
Key result
Duration:
28 d
Dose descriptor:
EC10
Effect conc.:
36.4 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Ter Munck
Remarks:
Haplic Luvisol
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
56.2 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Ter Munck
Remarks:
Haplic Luvisol
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Ter Munck
Remarks:
Haplic Luvisol
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
80.9 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Ter Munck
Remarks:
Haplic Luvisol
Key result
Duration:
28 d
Dose descriptor:
EC10
Effect conc.:
103.7 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Woburn
Remarks:
Dystric Cambisol
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Woburn
Remarks:
Dystric Cambisol
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
178 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Woburn
Remarks:
Dystric Cambisol
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
230.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Woburn
Remarks:
Dystric Cambisol
Key result
Duration:
28 d
Dose descriptor:
EC10
Effect conc.:
252 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Zegveld
Remarks:
Histosol
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
562 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Zegveld
Remarks:
Histosol
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Zegveld
Remarks:
Histosol
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
678.2 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
As (added dose)
Basis for effect:
reproduction
Remarks:
number of juveniles
Remarks on result:
other: Zegveld
Remarks:
Histosol
Results with reference substance (positive control):
not available
Reported statistics and error estimates:
Normality of the data:
checked with Kolmogorov-Smirnov or Shapiro-Wilk's test

Variance homogeneity of the data:
Levene's or Cochran test

NOEC and LOEC:
- In case data of normal distribution, homoscedasticity and a monotone dose-response relationship: Williams' Multiple Sequential t-test
- In case of normal distribution and variance heterogeneinty: one-sided Welch's t-test
- In case of normal distribution and homoscedasticity, but absence of a monotone relationship: Dunnett's t-test
- In case data were not normally distributed, but homoscedastic: Jonckheere-Terpstra test
- In case data were not normally distributed and heteroscedastic: Bonferroni U-test
- In case data were not normally distributed and the dose-response relationship was not monotone: Bonferroni Median test
(α = 0.05)

ECx values were determined with both linear and non-linear regression models
It was checked for the best fit (R2, Chi2, AIC) after performing an F-test to control significance of the dose response relationship
Validity criteria fulfilled:
yes
Conclusions:
The 28-d EC10 for the effect of arsenic to the reproduction of Eisenia fetida in six different soils ranged between 32.2 and 252.0 mg As/kg soil dry weight (based on added As concentrations in soil).

Description of key information

Fifteen reliable EC10 or NOEC values for chronic arsenic toxicity to reproduction of Folsomia candida were retrieved from 2 different studies. All data were derived from soils amended with disodium hydrogen arsenate salts. The reliable NOEC and EC10 values range between 20.0 and 320 mg As/kg dw, expressed based on added elemental As concentrations in soil. All reliable NOEC and EC10 values were taken forward to the derivation of the PNEC according to the statistical extrapolation approach.

Key value for chemical safety assessment

Additional information