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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / micronucleus study
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study based on scientific principles, sufficiently documented.

Data source

Reference
Reference Type:
publication
Title:
An investigation into the activation and deactivation of chlorinated hydrocarbons to genotoxins in metabolically competent human cells
Author:
Doherty, A.T. et al.
Year:
1996
Bibliographic source:
Mutagenesis, 11, 247-274

Materials and methods

Principles of method if other than guideline:
Method: other, details see below
GLP compliance:
not specified
Type of assay:
in vitro mammalian cell micronucleus test

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chlorohexane
EC Number:
208-859-5
EC Name:
1-chlorohexane
Cas Number:
544-10-5
Molecular formula:
C6H13Cl
IUPAC Name:
1-chlorohexane
Details on test material:
1-Chlorohexane, not further specified.

Method

Species / strainopen allclose all
Species / strain / cell type:
other: Human lymphoblastoid cell line AHH-1 (metabolically competent: esp. CYP1A1)
Species / strain / cell type:
other: Human lymphoblastoid cell line MCL (metabolically competent: esp. CYP1A1, derived from AHH-1)
Species / strain / cell type:
other: Human lymphoblastoid cell line h2E1 (metabolically competent: esp. CYP2E1)
Metabolic activation:
without
Test concentrations with justification for top dose:
nominal: 1.0, 2.5, 5.0, and 10.0 mM (12 - 120 ug/ml)

Results and discussion

Test resultsopen allclose all
Metabolic activation:
without
Genotoxicity:
ambiguous
Cytotoxicity / choice of top concentrations:
other: 2.5 mM (30 ug/ml)
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
other: 2.5 mM (30 ug/ml)
Remarks on result:
other: other: Human lymphoblastoid cell line AHH-1 (metabolically competent: esp. CYP1A1)
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

All compounds except hexachloroethane produced dose-related increases in the

frequency of micronuclei.

Human lymphoblastoid cell line AHH-1 (metabolically competent: esp. CYP1A1):
1-Chlorohexane showed a significant increase at 5 and 10 mM, but not at 1.0 and

2.5 mM, 2.2 and 3.7-fold above background, respectively. The result proved to be

reproducible in either parallel test.

Human lymphoblastoid cell line MCL (metabolically competent: esp. CYP1A1, derived from AHH-1):

1-Chlorohexane showed a significant increase at 2.5 to 10 mM, but not at 1.0 mM,

3.3 to 4.7-fold above background, respectively. The result proved to be

reproducible in either parallel test.

Human lymphoblastoid cell line h2E1 (metabolically competent: esp. CYP2E1):

1-Chlorohexane showed a significant increase at 2.5 to 10 mM, but not at 1.0 mM,

3.2 to 4.4-fold above background, respectively. The result proved to be

reproducible in either parallel test.



The 1-chlorohexane-induced micronuclei were classified as kinetochor- and

centromer-negative types indicating a clastogenic rather than aneugenic mechanism

in mitosis.
  

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive without metabolic activation