2-(2-(2-butoxyethoxy)ethoxy)ethanol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report which meets basic scientific principles

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1962

Reference 2

Reference Type:

publication

Title:

Glycol Ethers and Other|SelectedGlycol Derivates"; in Clayton G.D and Clayton F.E.|(eds.): Patty's Industrial Hygiene and Toxicology, 4th ed.

Author:

Smyth H.F. et al.

Year:

1994

Bibliographic source:

Am. Ind. Hyg. Assoc. J., 23, 95, (1962);|cited in Gingel R. et al.:Clayton F.E.|(eds.): Patty's Industrial Hygiene and Toxicology, 4th ed.,|Vol.2, Part D, pp. 2761-2964, (1994)

Reference 3

Reference Type:

review article or handbook

Title:

Doc.Nr. 878216157, NTIS/OTS 0206832

Author:

EPA

Year:

1994

Bibliographic source:

TSCATS EPA/OTS Doc.Nr. 878216157, NTIS/OTS 0206832

Reference 4

Reference Type:

study report

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Inhalation hazard test according to the method described by Smyth H.F. et al.: Am. Ind. Hyg. Ass. J. 23, 95-107, (1962).

Principles of method if other than guideline:

Inhalation hazard test according to the method described by Smyth H.F. et al.: Am. Ind. Hyg. Ass. J. 23, 95-107, (1962). See details below.

GLP compliance:

no

Test type:

other: Inhalation Hazard Test

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-(2-butoxyethoxy)ethoxy)ethanol

EC Number:

205-592-6

EC Name:

2-(2-(2-butoxyethoxy)ethoxy)ethanol

Cas Number:

143-22-6

Molecular formula:

C10H22O4

IUPAC Name:

2-[2-(2-butoxyethoxy)ethoxy]ethanol

Details on test material:

Name of test material (as cited in study report):
- Analytical purity: 99% (pure)
Triethylene glycol monobutyl ether

Test animals

Species:

rat

Strain:

other: Carworth Wistar albino rats

Sex:

male/female

Details on test animals or test system and environmental conditions:

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 55 ± 5
- Photoperiod (hrs dark / hrs light): 12h / 12h

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Male or female rats were exposed to a flowing stream of vapor-ladened air generated by passing 2.5 l/min of dried air at room
temperature through a fritted disc immersed to a depth of at least once inch in approximately 50 ml of test material contained in a
gas-washing bottle.
Rats were exposed from time periods ranging from 15 minutes to 8 hours (until the inhalation period killing about one half of the rats
within 14 days was defined).

Analytical verification of test atmosphere concentrations:

no

Remarks:

No analytical determination of the atmosphere concentrations was performed.

Duration of exposure:

8 h

Concentrations:

4 hr: 600mg/l
8 hr: 1200mg/l

No. of animals per sex per dose:

6

Control animals:

no

Details on study design:

Duration of observation period following administration: 14 days
- Other examinations performed: clinical signs, histopathology

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LCLo

Effect level:

1 200 mg/m³ air

Based on:

test mat.

Exp. duration:

8 h

Effect levels 2

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 2 400 mg/m³ air

Based on:

test mat.

Exp. duration:

16 h

Remarks on result:

other: estimated LC50 from available data

Effect levels 3

Sex:

male/female

Dose descriptor:

other: NOAEC

Effect level:

480 mg/m³ air

Based on:

test mat.

Exp. duration:

16 h

Remarks on result:

other: estimated NOAEC from available data

Mortality:

Rats were exposed from time periods ranging from 15 minutes to 8 hours (until the inhalation period killing about one half of the rats within 14 days was defined).
No mortality was observed after 8 h exposure.

Clinical signs:

other: no significant adverse effects.

Body weight:

Body weights were not determined neither during the exposure nor at the end of the test.

Gross pathology:

The animals that were sacrificed and submitted to necropsy at the end of the observation period were without findings.

Any other information on results incl. tables

Exposure	Mortality Dead/Treated
8h	0/6
16h	3/6

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

In an acute inhalation toxicity designed to assess vapour concentrations that did not cause death, groups of 6 animals were exposed sequentially to increasing concentrations of 2-(2-(2-butoxyethoxy)ethoxy)ethanol.
8 hours exposure to 1200mg/l caused no deaths whereas 16 hours at 2400mg/l caused 3/6 animals to die. Based on this study, the LC50 would be >2400 mg/l.
It can be concluded that the test substance does not present an acute inhalation hazard.

Executive summary:

In an acute inhalation toxicity designed to assess vapour concentrations that did not cause death, groups of 6 animals were exposed sequentially to increasing concentrations of 2-(2-(2-butoxyethoxy)ethoxy)ethanol.

8 hours exposure to 1200mg/l caused no deaths whereas 16 hours at 2400mg/l caused 3/6 animals to die. Based on this study, the LC50 would be >2400 mg/l.

It can be concluded that the test substance does not present an acute inhalation hazard.