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Toxicity to microorganisms

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Description of key information

3 h EC50 (OECD 209): 1204 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
1 204 mg/L

Additional information

In the Klimisch 1 GLP study from Fiebig (2013) the respiration inhibition of Aspartic acid, N-(3-carboxy-1-oxo-sulfopropyl)-N-(C16-C18 (even numbered), C18 unsaturated alkyl) tetrasodium salts on activated municipal sludge was determined according to OECD 209. Based on the outcome of a pre-test, the test was performed at following concentrations Control, 62.5 - 125 - 250 - 500 - 1000 mg/L. The test groups were set up in with following number of replicates: 6 (Control), 5 (62.5, 125, 250 mg/L), 4 (500 mg/L) and 1 (1000 mg/L). Except the lower number of replicates in the two higher test concentrations, all validity parameters as outlined in the OECD 209 were fulfilled. The lower number of replicates in the two higher test concentrations which was chosen due to the limited amount of test item, is considered to have no impact on the outcome of the study, especially since the outcome is furthermore supported by the range-finding test. Statistical evaluation showed that the test item has an EC50 of > 1000 mg/L, extrapolated to 1204 mg/L.

The results of this study are considered relevant and reliable for the risk assessment.