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EC number: 236-740-8 | CAS number: 13472-08-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labeling and/or risk assessment.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Remarks:
- The study was conducted according to guideline in effect at time of study conduct.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- One sample plus a backup sample was received from each test concentration, including the dilution water control on day 0 of the test before the test solutions were poured into the replicate test chambers. One sample plus a backup sample was also received from 2 of the 4 replicate chambers (replicates B and D) at the nominal 120 mg/L test concentration on day 1 due to total immobility in the test concentration. One sample plus a backup sample was also received from 2 of the 4 replicate chambers (replicates B and D) at the nominal 7.5, 15, 30 and 60 mg/L test concentrations and the dilution water control at test end (day 2). All samples and backup samples were stored refrigerated when not in use.
Concentrations of the active substance in diluted samples were measured by high performance liquid chromatography with detection by ultraviolet (UV) spectroscopy (LC/UV) within 2 days of sample receipt. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
Test substance solutions were prepared by dilution from a stock solution in Haskell well water (HWW). A 120 mg/L stock solution was prepared by adding approximately 0.240 g of test material to HWW in a 2-L glass beaker (final volume 2000 mL), stirring for approximately 5 minutes, sonicating for approximately 13 minutes, and stirring for an additional 17 minutes. The stock solution was also the highest test concentration. Test solutions were prepared by adding the appropriate volume of the stock solution to the appropriate volume of HWW in 1-L glass beakers (final volume 1000 mL) and stirring for approximately 18 minutes. The nominal 120 mg/L stock solution (and highest test solution), and the 30 and 60 mg/L test solutions were clear and colourless with no visible precipitate and had a surface film. The dilution water control and the nominal 7.5 and 15 mg/L test solutions were clear and colourless with no visible precipitate or surface film. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM:
- Strain: Daphnia magna
- Age at study initiation: < 24 hours
- Feeding during test: None
ACCLIMATION
- Acclimation period: Neonates used in this test were less than 24 hours old and were collected from the 6th brood of 21-day old parent daphnids. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- - Total alkalinity: 73 to 82 mg/L as CaCO3
- EDTA hardness: 138 to 167 mg/L as CaCO3 - Test temperature:
- 20.0 to 20.3 °C
- pH:
- 8.3 to 8.4
- Dissolved oxygen:
- 8.5 to 9.0 mg/L
- Salinity:
- freshwater
- Nominal and measured concentrations:
- Nominal concentrations: 0, 7.5, 15, 30, 60, and 120 mg/L
Measured concentrations: 0, 8.20, 16.7, 34.4, 78.4, and 132 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (250-mL) containing approximately 200 mL of test solution
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Conductivity: 278 to 299 µmhos/cm
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: a photoperiod of 16 hours light and 8 hours darkness was employed, which included 30 minutes of transitional light preceding and following the 16-hour light interval.
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations of test organisms were made daily. The criterion for the effect (immobility) was the inability to swim within 15 seconds after gentle agitation of the test vessel or test solution (even if the daphnids can still move their antennae).
TEST CONCENTRATIONS
- Range finding study: A range-finding study with 1 test chamber containing 10 organisms per test substance concentration was conducted using a dilution water control and nominal test substance concentrations of 0.12, 1.2, 12, and 120 mg/L. At the end of the 48 hours, the respective immobility values were 0, 0, 0, 0, and 100%. All test substance solutions except the nominal 120 mg/L concentration were clear and colourless with no visible precipitation at test start. The nominal 120 mg/L test substance concentration was clear and colourless with undissolved test substance present. - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 51.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: confidence intervals of 34.4 to 78.4 mg/L.
- Details on results:
- Six daphnids were lethargic at 24 hours in the mean, measured 78.4 mg/L concentration.
The highest mean, measured concentration causing no immobility at test end was 34.4 mg/L. The lowest mean, measured concentration causing 100% immobility at test end was 78.4 mg/L.
The 24-hour EC50, based on mean, measured concentrations of the test substance and immobility, was 83.4 mg/L with 95% confidence intervals of 68.8 to 99.4 mg/L. - Reported statistics and error estimates:
- The 24- and 48-hour EC50 values were calculated by the moving average angle and the binomial methods respectively, based on mean, measured concentrations of the test substance and immobility. The highest mean, measured concentration causing no immobility at test end and the lowest mean, measured concentration causing 100% immobility at test end were assessed by visual observation.
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study and the conclusions which are drawn from it fulfill the quality criteria (validity, reliability, repeatability).
48-hour EC50 = 51.9 mg /L. - Executive summary:
The acute toxicity of the test substance to unfed Daphnia magna neonates, less than 24 hours old at test start, was determined in an unaerated, 48-hour, static test. The test was conducted in accordance with the OECD Guideline for the Testing of Chemicals 202.
The study was conducted with 5 concentrations and a dilution water control at a mean temperature of 20.2 ºC. Four replicates with 5 daphnids per replicate were used per test substance concentration and dilution water control. Exposure of daphnids to nominal concentrations of 7.5, 15, 30, 60, and 120 mg/L (which correspond to measured concentrations of 8.20, 16.7, 34.4, 78.4, and 132 mg/L) resulted in 0, 0, 0, 100, and 100% immobility, respectively, at the end of 48 hours.
The 48-hour EC50, based on mean, measured concentrations of the active substance and immobility, was 51.9 mg/L.
Reference
Table 1: Immobility Data
Mean, Measured Concentration (mg/L) |
Number Immobile / Number at Test Start |
|||||||
24 Hours |
48 Hours |
|||||||
A |
B |
C |
D |
A |
B |
C |
D |
|
Dilution Water Control |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
8.20 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
16.7 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
34.4 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
78.4 |
0/5 |
4/5 |
0/5 |
0/5 |
5/5 |
5/5 |
5/5 |
5/5 |
132 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
5/5 |
Description of key information
A static, 48-hour study in Daphnia magna with the test substance was performed. The 48-hour LC50 in Daphnia magna was 51.9 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 51.9 mg/L
Additional information
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