Cyclopropanemethanol, 1-methyl-2-[(1,2,2-trimethylbicyclo[3.1.0]hex-3-yl)methyl]-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 March 1998 - 03 July 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

17 July, 1992.

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

December, 1992

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

JAVANOL :
Batch No. : 008825
Purity : 88.4% (sum of the two peaks)
Expiry Date : 18 February, 2000

Analytical monitoring:

yes

Remarks:

GC-FID

Details on sampling:

Static set-up. Samples were taken at the beginning and end of the exposure.

Further samples were taken from the solutions at 56 and 100% after all fish were dead.

Vehicle:

yes

Remarks:

Acetone at maximum of 0.1 mL/L.

Details on test solutions:

A stock solution in acetone of JAVANOL was prepared at 100 mg/mL. Per litre of stock medium 0.1 mL of this stock was added and the solution stirred overnight. Prior to testing, this solution was filtered through a 0.2 micron filter to obtain the actual dissolved fraction. The filtrate was a clear, colourless solution. This filtrate was further diluted to prepare the final test concentrtions :
100% filtrate
56% filtrate
32% filtrate
18% filtrate
10% filtrate
Control
Solvent Control (acetone at 0.1 mL/L)

Details on test organisms:

Carp (C. carpio)
Mean length : 2.10 +/- 0.10 cm
Mean weight : 0.25 +/- 0.06 g
Quarantined for at least 12 days post delivery
Medium : ISO Medium
Validity of Batch : Mortality during the 7 days prior to the start of the study was less than 5%

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

250 mg CaCO3/L

Test temperature:

20.0 to 20.7°C

pH:

pH range 7.6 - 8.0

Dissolved oxygen:

Dissolved O2 Range = 7.1 - 9.3 mgO2/L

Nominal and measured concentrations:

Exposure concentrations were determined at Time 0 and at the end of the exposure (96 hours) :

Filtrate % Time 0 (mg/L) 96 hour (mg/L)
100% 4.96 3.95 (at 24 hours - all fish dead)
56% 2.79 1.97 (at 24 hours - all fish dead)
32% 1.55 0.479
18% 0.840 0.181
10% 0.496 0.0784

Details on test conditions:

Test Duration : 96 hours
Test Type : Static
Test Vessels : 8 Litres all glass (with 5 L of test medium)
Test Medium : ISO Medium
No. of Fish : 7 fish per concentration and controls
Fish Loading : Approx. 0.35 g/L (7 fish per 5 litres)
Photoperiod : 16 hours per day of light / 8 hours dark
Aeration : No aeration
Feeding : No feeding 48 hours prior to start of test

Reference substance (positive control):

yes

Remarks:

Pentachloropehnol

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.02 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortality observed in the different exposure concentrations at 96-hours :
Control 0%
Solvent-Control 0%
10% Filtrate (Mean Exp. 0.195 mg/L) 0%
18% Filtrate (Mean Exp. 0.390 mg/L) 0%
32% Filtrate (Mean Exp. 0.862 mg/L) 14%
56% Filtrate (Mean Exp. 2.34 mg/L) 100% (all dead by 24 hours)
100% Filtrate (Mean Exp. 4.43 mg/L) 100% (all dead by 24 hours)

Results with reference substance (positive control):

Pentachlorophenol :
LC50 decreased from 0.16 mg/L (95% CI : 0.14 - 0.21 mg/L) at 24 hours to 0.13 mg/L (95 % CI : 0.12 - 0.17 mg/L).

Validity criteria fulfilled:

yes

Conclusions:

An acute fish toxicity study was performed with Common Carp exposed to JAVANOL. The following end-points were determined, based on geometric mean measured concentrations :

96 hour LC50 = 1.02 mg/L
96 hour LC0 = 0.4 mg/L
96 hour LC100 = 2.3 mg/L

Description of key information

An acute fish toxicity study was performed with Common Carp exposed to JAVANOL. The following end-points were determined, based on geometric mean measured concentrations :

96 hour LC50 = 1.02 mg/L

96 hour LC0 = 0.4 mg/L

96 hour LC100 = 2.3 mg/L

.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.02 mg/L

Additional information

An acute fish toxicity study was performed with Common Carp exposed to JAVANOL. The following end-points were determined, based on geometric mean measured concentrations :

96 hour LC50 = 1.02 mg/L

96 hour LC0 = 0.4 mg/L

96 hour LC100 = 2.3 mg/L