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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From July 29 to September 6, 2005
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
This study was performed according to OECD Guideline 121 and EU Method C.19 with GLP statement. This study is well documented and all validity criteria were fulfilled.
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Principles of method if other than guideline:
Not applicable
GLP compliance:
yes (incl. certificate)
Remarks:
UK GLP Compliance Programme (inspected on April 12, 2005/ signed on June 01, 2005)
Type of method:
HPLC estimation method
Media:
soil
Specific details on test material used for the study:
PHYSICO-CHEMICAL PROPERTIES
- Vapour pressure: 0.098 Pa at 25 °C
- Relative density: 1.07
- Surface tension: 46.5 mN/m (90% saturated solution)
- Auto-ignition temperature: None below 400 °C
- Oxidising properties: Non-oxidising
- Explosive properties: Not explosive
Radiolabelling:
no
Test temperature:
25 °C
Details on study design: HPLC method:
EQUIPMENT & MOBILE PHASE
Instrument: Hewlett Packard 1050 Liquid Chromatograph
Column: Hypersil H5CPS (25 cm x 4.6 mm internal diameter)
Column temperature: 25°C
Mobile phase composition: Methanol:water (55:45 v/v)
Flow rate: 1.0 mL/min
Injection volume: 10 µL
Detector: UV set at 220 nm

DETERMINATION OF DEAD TIME
- An internal standard (formamide) with no retention on the HPLC column was used to determine the deadtime of the HPLC system.

REFERENCE SUBSTANCES
- Formamide (Internal deadtime standard)
- Acetanilide
- Nitrobenzene
- Naphthalene
- 2-Methylnaphthalene
- Anthracene
- DDT

DETERMINATION OF RETENTION TIMES
- Formamide (Internal deadtime standard), Log10Koc not applicable (deadtime only), Conc. 796 mg/L
- Acetanilide, Log10Koc 1.25, Conc. 20.5 mg/L
- Nitrobenzene, Log10Koc 2.36, Conc. 21.3 mg/L
- Naphthalene, Log10Koc 2.75, Conc. 2.12 mg/L
- 2-Methylnaphthalene, Log10Koc 3.87, Conc. 1.96 mg/L
- Anthracene, Log10Koc 4.41, Conc. 39.2 mg/L
- DDT, Log10Koc 5.63, Conc. 82.6 mg/L

REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k: t’r / to = (tr – to) / to
- Determination of the log Koc value: Log10Koc = 2.81 Log10k + 4.02
Analytical monitoring:
yes
Details on sampling:
see above
Details on matrix:
not applicable
Details on test conditions:
not applicable
Key result
Type:
log Koc
Value:
2.7 dimensionless
Temp.:
25 °C
Key result
Type:
Koc
Value:
510 L/kg
Temp.:
25 °C
Details on results (HPLC method):
See the "Attached background material section" for information on table of results
Statistics:
None

None

Validity criteria fulfilled:
yes
Conclusions:
Koc = 510 L/Kg ; log Koc = 2.7.

Executive summary:

The determination of the adsorption coefficient of the test material was performed according to the OECD Guideline No. 121/ EU Method C. 19 with GLP statement.

The experimental method uses HPLC for the estimation of the adsorption coefficient Koc in soil. An analytical column Hypersil H5CPS (25 cm x 4.6 mm internal diameter) was used, with a column temperature at 25 °C. The mobile phase was methanol: water (55:45 v/v). The test item was prepared in mobile phase (18.7 mg/L). The dead time was determined by measuring the retention time of formamide, diluted in mobile phase. The following reference mixture was prepared in HPLC mobile phase: Acetanilide, Nitrobenzene, Naphthalene, 2-Methylnaphthalene, Anthracene and DDT. A solution of test item (18.7 mg/L in mobile phase) was prepared and chromatographed in duplicate, bracketed by samples of the reference mixture, using the HPLC.

In the present study, the reference items covered the range of log Koc 1.25 to 5.63.

Under the conditions of the test, the retention time of the test item was found at 4.340 min for the first injection and at 4.328 min for the second injection. The corresponding log Koc was 2.7 (Koc = 510 L/Kg).

Description of key information

OECD Guideline 121, GLP, key study, validity 1:

Koc = 510 at 25 °C

LogKoc = 2.7 at 25°C

Key value for chemical safety assessment

Koc at 20 °C:
510

Additional information

The determination of the adsorption coefficient of the test material was performed according to the OECD Guideline No. 121/ EU Method C. 19 with GLP statement.

The experimental method uses HPLC for the estimation of the adsorption coefficient Koc in soil. An analytical columnHypersil H5CPS (25 cm x 4.6 mm internal diameter) was used, with a column temperature at 25 °C. The mobile phase was methanol: water (55:45 v/v). The test item was prepared in mobile phase (18.7 mg/L). The dead time was determined by measuring the retention time of formamide, diluted in mobile phase. The followingreference mixture was prepared in HPLC mobile phase: Acetanilide, Nitrobenzene, Naphthalene, 2-Methylnaphthalene, Anthracene and DDT.A solution of test item (18.7 mg/L in mobile phase) was prepared and chromatographed in duplicate, bracketed by samples of the reference mixture, using the HPLC.

In the present study, the reference items covered the range of log Koc 1.25 to 5.63.

Under the conditions of the test, the retention time of the test item was found at 4.340 min for the first injection and at 4.328 min for the second injection. The corresponding log Koc was 2.7 (Koc = 510).

[LogKoc: 2.7]