1,3-bis(3-methyl-2,5-dioxo-1H-pyrrolinylmethyl)benzene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study was done under GLP and is valid, however the analysis is questionable

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Method C2 of 84/449/EEC OECD Guideline 202

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

88% pure according to CoA

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: none

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

211 mg CaCO3/L

Test temperature:

17.5-19.5

pH:

7.6-8.4

Dissolved oxygen:

96-100% ASV

Nominal and measured concentrations:

nominal: 0, 0.75, 1.5, 3, 6, 12 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

2.69 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.06 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.743 mg/L

Details on results:

%Concentration loss over test: 0 ... 99

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Study is valid Ec50 was determined to be 2.06 mg/L