(2E)-2-methyl-3-phenylacrylaldehyde

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

No exact guideline or method detailed in report.

GLP compliance:

not specified

Remarks:

pre-GLP report

Test type:

fixed dose procedure

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

(E)-2-methyl-3-phenylprop-2-enal

Cas Number:

15174-47-7

IUPAC Name:

(E)-2-methyl-3-phenylprop-2-enal

Test material form:

liquid

Specific details on test material used for the study:

Test material was described with the generic name "alpha-methylcinnamaldehyde". As manufactured by Innospec, the product met the criteria of > 80% trans-isomer, supporting that the test material was the isomer better described as CAS 15174-47-7.

Test animals

Species:

rat

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

Compound No. RIFM 72-257

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

not specified

Doses:

1.64, 2.05, 2.56, 3.20, 5.00 g/Kg

No. of animals per sex per dose:

10 animals per dose, no sex details given

Control animals:

not specified

Results and discussion

Effect levels

Key result

Sex:

not specified

Dose descriptor:

LD50

Effect level:

ca. 2 050 mg/kg bw

95% CL:

> 1.83 - < 2.27

Mortality:

On day 1 post oral exposure mortality was observed as follows:
1.64 g/Kg - 2 rats dead
2.05 g/Kg - 4 rats dead
2.56 g/Kg - 8 rats dead
3.20 g/Kg - 10 rats dead
5.00 g/Kg - 10 rats dead

Clinical signs:

other: The animals which died experienced sluggishness prior to death

Any other information on results incl. tables

Table 1. Distribution of Mortality
Dose (g/Kg)	Observation Day														Mortality
	1	2	3	4	5	6	7	8	9	10	11	12	13	14
1.64	2	0	0	0	0	0	0	0	0	0	0	0	0	0	2/10
2.05	4	0	0	0	0	0	0	0	0	0	0	0	0	0	4/10
2.56	8	0	0	0	0	0	0	0	0	0	0	0	0	0	8/10
3.20	10	0	0	0	0	0	0	0	0	0	0	0	0	0	10/10
5.00	10	0	0	0	0	0	0	0	0	0	0	0	0	0	10/10

Applicant's summary and conclusion

Interpretation of results:

other: Not classified

Remarks:

Regulation (EC) No 1272/2008 on classification, labelling and packaging (CLP) of substances and mixtures

Conclusions:

Based on observed mortality, the acute oral LD50 of Alpha methyl cinnamic aldehyde was determined to be 2050 mg/Kg (95% confidence interval = 1830 – 2270 mg/Kg).

Executive summary:

In a key fixed dose oral toxicity study, the test material (Alpha methyl cinnamic aldehyde) was orally administered to rats (10/dose) at doses of 1.64, 2.05, 2.56. 3.20, or 5.0 g/Kg.

 

All animals treated at ≥3.20 g/Kg died on day one. At 1.64 g/Kg, 2/10 animals died while 4/10 and 8/10 animals died post exposure to the test material at doses of 2.05 and 2.56 g/Kg, respectively.

 

The acute oral LD₅₀ of Alpha methyl cinnamic aldehyde was therefore determined to be 2050 mg/Kg (95% confidence interval = 1830 – 2270 mg/Kg).