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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to fish:

Study was conducted to investigate the acute toxicity of the test chemical on the mortality of Pimephales promelas (Fathead Minnow). Study conducted for 96-hour under the static system. Test chemical 40.5 g/l were used and prepared by stirring. Test chemical analytically determined by Gas liquid chromatography. Test performed on 48-50 days old Pimephales promelas. Nominal concentrations selected for the study was 0, 1.88, 3.75, 7.50, 15 and 30 mg/l. Based on the mortality of test organism Fathead Minnow by the chemical exposure for 96 hrs, the LC50 was determine to be 15.6 mg/l. Based on the LC50 value, chemical was consider as toxic and can be consider to be classified in aquatic chronic 3 as per the CLP classification criteria. The chemical was readily biodegradable in water, thus before exposure with the test fish, chemical degrades rapidly. Thus on that criteria, chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

The objective of the present study was to determine the toxicity of test chemical on the mobility of aquatic invertebrate Daphnia magna. Test conducted in accordance with American society for testing and Materials (ASTM). Daphnia magna maintain in the appropriate system and follows all the criteria which meet the guideline. Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID. 0-24 hrs old Daphnia magna were used in the study. Organisms have not fed 24 hrs before and during the test. Test conducted in 250 ml beakers which have two 1.9 cm holes located 1 cm from the bottom and on opposite sides of each beaker. 20 Daphnia per concentration were added and exposed for 48 hrs. Adult Daphnids were separated into 2 liter battery jars. After 24 hrs, 0-24 hrs old Daphnia were carefully collected using fire polished glass tubes and squeeze bulbs, counted into 250 ml exposure beakers which were randomly hung in the 10 × 30 cm compartments of the 12 exposure tanks. After 48 hrs immobility and dead organisms were measured by dissecting microscope. Based on the immobility of Daphnia magna by the chemical exposure for 48 hrs, the EC50 was determine to be at 3.25 mg/l with the 95 % CI of 2.72-3.88 mg/l. Based on the EC50 value, chemical consider as toxic and classified in aquatic chronic category 2 but as the test chemical degrades rapidly in water, thus on the basis of readily biodegradability criteria, test chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

After the exposure of test substance with freshwater algae Selenastrum capricornutum, effects on biomass and growth rate were observed. Based on the biomass inhibition, EC50 was determine to be at 115 mg/l and on the basis of growth rate inhibition the EC50 was determine to be at 72.9 mg/l. The NOEC was observed at 10 mg/l. As the test chemical degrades rapidly in water, thus on the basis of readily biodegradability criteria, test chemical consider to be nontoxic and not classified as per the CLP classification criteria. 

Additional information

Short term toxicity to fish:

Based on the various experimental data from peer reviewed journal, studies were carried out for the determination of toxicity of test chemical on the mortality rate of fishes. The studies are as mentioned below:

 

Study was conducted to investigate the acute toxicity of the test chemical on the mortality of Pimephales promelas (Fathead Minnow). Study conducted for 96-hour under the static system. Test chemical 40.5 g/l were used and prepared by stirring. Test chemical analytically determined by Gas liquid chromatography. Test performed on 48-50 days old Pimephales promelas. Nominal concentrations selected for the study was 0, 1.88, 3.75, 7.50, 15 and 30 mg/l. Based on the mortality of test organism Fathead Minnow by the chemical exposure for 96 hrs, the LC50 was determine to be 15.6 mg/l. Based on the LC50 value, chemical was consider as toxic and can be consider to be classified in aquatic chronic 3 as per the CLP classification criteria. The chemical was readily biodegradable in water, thus before exposure with the test fish, chemical degrades rapidly. Thus on that criteria, chemical was consider as nontoxic and not classified as per the CLP classification criteria.

 

Similar study was conducted to investigate the acute toxicity of the test chemical to Pimephales promelas was determined for a 96-hour. Test conducted under the static system according to the standards. Chemical analytically determined by Gas liquid chromatography. Test chemical 100 mg/l were used and prepared by stirring. Study conducted on the nominal concentrations 0, 2.96, 4.56, 7.02, 10.8 and 16.6 mg/l. 29 days old Pimephales promelas was used a test organism. In 2 liter tank, different concentrations were added with 0.850 g/l Pimephales promelas. After the exposure period of 96 hrs, affected fish lost schooling behavior, were hyper- active and swam near the tank surface. They were over- reactive to external stimuli, had increased respiration, and darkly colored. Also had spinal deformities and lost equilibrium prior to death. Based on the mortality of test organism by the chemical exposure for 96 hrs, the LC50 value was determine to be 12.2 mg/l. Based on the LC50 value, chemical consider as toxic and can be consider to be classified in aquatic chronic category 3, but as the chemical was readily biodegradable in water, thus can be consider to be nontoxic and not classified as per the CLP classification criteria.

 

Above study was further supported by the third study from peer reviewed journal. The objective of this study was to develop procedures that relate modes of acute toxic action of test chemical on the fathead minnow (Pimephales promelas). Short term toxicity study to Fathead Minnow (Pimephales promelas) was carried out for 96 hrs. Test was conducted using Lake Superior water at 25±1°C. Aqueous toxicant concentrations were measured in tests with quality assurance criteria requiring 80% agreement between duplicate samples and 90 to 110% spike recovery. Flow-through exposures were conducted using cycling proportional, modified Benoit, or electronic diluters. The test was performed under the flow-through condition with temp of 25 ± 1°C. 28 - 30 d old juvenile test organisms were used for the study. Median lethal concentration (LC50) was calculated using the Trimmed Spearman–Karber Method, with 95% confidence intervals being calculated when possible. Fish shows type III syndrome which indicates spontaneous locomotor activity, convulsion, spasms, tetany, scoliosis, lordosis, and/or hemorrhaging in the vertebral column. After the exposure of 96 hrs of chemical with the Fathead Minnow (Pimephales promelas), the LC50 was observed to be 13.8 mg/l. Based on the LC50 value, test chemical was consider as toxic and classified in chronic 3 category. But as the chemical was readily biodegradable in water thus considering that criteria, chemical consider to be nontoxic and not classified as per the CLP classification criteria.

 

Another experimental study from peer reviewed journal was conducted to investigate the effect of test chemical on the mortality rate of fishes Pimephales promelas and Carassius auratus. Test conducted according to the OECD guideline 203 and test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline. Chemical analyzed with a Hewlett-Packard 5840A gas chromatograph equipped with model 7671 A auto injector and also with FID. Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Organisms does not fed 24 hrs before and during the test. Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top. Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used. As the test conducted on the two species of fish different effect concentrations were obtain at which 50 % mortality were observed. Based on the mortality of Pimephales promelas (Fathead Minnow) and Carassius auratus (Goldfish) by the exposure of test chemical for 96 hrs, the LC50 value was determine to be at 19.7 mg/l and 13.1 mg/l. Based on the LC50 value, test chemical was consider as toxic and classified in aquatic chronic 3 category, but as the test chemical was readily biodegrades in water, thus on that criteria chemical consider as nontoxic and not classified as per the CLP classification criteria.

 

Similar fifth study was conducted to determine the effect of test chemical on the mortality rate of fishes Oncorhynchus mykiss (Rainbow Trout) and Lepomis macrochirus (Bluegill). Test conducted for 96 hours and according to the OECD guideline 202. Test organisms were maintain in the appropriate system and follows all the criteria which meet the guideline. Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID. Both fishes obtain from the stock-culture units of the Environmental Research Laboratory-Duluth. Fishes not fed 24 hrs before and during the test. Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top. Test performed in duplicates with control which simultaneously run with the test chemical. Effect calculated by Trimmed Spearman- Karber method were used. Based on the mortality of Oncorhynchus mycoses (Rainbow Trout) and Lepomis macrochirus (Bluegill) by the exposure of test chemical for 96 hrs, the LC50 was determine to be at 7.31 mg/l and 4.31 mg/l. Based on the LC50 value, test chemical was consider as toxic and classified as chronic 2 category. Test chemical degrade faster in water and if exposed in water degrades faster and the chance of toxicity to fishes was les. Thus based on the readily biodegradability criteria of test chemical, it can be consider that the chemical was nontoxic and not classified as per the CLP classification criteria.

Based on the above studies, it can be concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Based on the various experimental data from peer reviewed journal, studies were carried out for the determination of toxicity of test chemical on the mortality and mobility of aquatic invertebrates. The studies are as mentioned below:

 

The objective of the present study was to determine the toxicity of test chemical on the mobility of aquatic invertebrate Daphnia magna. Test conducted in accordance with American society for testing and Materials (ASTM). Daphnia magna maintain in the appropriate system and follows all the criteria which meet the guideline. Chemical analyzed with a Hewlett-Packard 5840 A gas chromatograph equipped with model 7671 A auto injector and also with FID. 0-24 hrs old Daphnia magna were used in the study. Organisms have not fed 24 hrs before and during the test. Test conducted in 250 ml beakers which have two 1.9 cm holes located 1 cm from the bottom and on opposite sides of each beaker. 20 Daphnia per concentration were added and exposed for 48 hrs. Adult Daphnids were separated into 2 liter battery jars. After 24 hrs, 0-24 hrs old Daphnia were carefully collected using fire polished glass tubes and squeeze bulbs, counted into 250 ml exposure beakers which were randomly hung in the 10 × 30 cm compartments of the 12 exposure tanks. After 48 hrs immobility and dead organisms were measured by dissecting microscope. Based on the immobility of Daphnia magna by the chemical exposure for 48 hrs, the EC50 was determine to be at 3.25 mg/l with the 95 % CI of 2.72-3.88 mg/l. Based on the EC50 value, chemical consider as toxic and classified in aquatic chronic category 2 but as the test chemical degrades rapidly in water, thus on the basis of readily biodegradability criteria, test chemical consider to be nontoxic and not classified as per the CLP classification criteria. 

 

First study was supported by the second study from the peer reviewed journal. Principle of this study was to determine the toxicity of test chemical on the mortality of Aplexa hypnorum (Snail). Test conducted according to the American society for testing and Materials (ASTM). Aplexa hypnorum (Snail) maintain in the appropriate system and follows all the criteria which meet the guideline. Chemical analyzed with a Hewlett-Packard 5840A gas chromatograph equipped with model 7671 A auto injector and also with FID. Adult Aplexa hypnorum (Snail) obtain from the stock-culture units. Organisms have not fed 24 hrs before and during the test. Test conducted in glass aquaria tank 60×30×30 cm deep. Tank divided into six compartments and one wide compartment with stainless steel screen divider. One standpipe were also present at the top. After 96 hrs. dead snail were measured by dissecting microscope. Based on the mortality of Aplexa hypnorum snail by the chemical exposure for 96 hrs, the EC50 was determine to be > 38.2 mg/l. On the basis of EC50 value, chemical was consider as toxic and classified in aquatic chronic 3 category, but on the basis of readily biodegradability nature of test chemical, it was concluded that the chemical does not last for longer period of time in water and consider to be nontoxic and not classified as per the CLP classification criteria.

 

Based on the above studies, it can be concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.

 

Toxicity to aquatic algae and cyanobacteria:

Short term toxicity study of test chemical on aquatic algae was conducted for 96 hrs. Test performed in accordance with OECD guideline 201. Chemical was analytically monitored by HPLC and spectrophotometer. 10,000 mg/l test compound dissolved in sterile ASTM (American Society for Testing and Materials) Type II water and prepared stock solutions with different concentrations 0, 10, 20, 40, 80, and 160 mg/l. 125-ml Erlenmeyer flasks were used producing a final volume of 50 ml with 1-ml aliquot of a concentrated cell suspension (7.2 × 104cells/ml). Algal growth was measured spectrophotometrically (at 350 nm) in the interval of 24 hours using filtered medium as a blank. Correct appearance of the algal cells was verified by microscopic examination before testing. A range-finding test (0, 0.1, 1.0, 10, 100, and 1,000 mg/L) was conducted to determine the median effective concentration (EC50) and the median effective concentrations for inhibition of algal growth (ErC50) and biomass accumulation (EbC50). Thus based on the range finding study definitive concentration were selected. Culture was maintained in algal medium at 24 ± 2 °C with illumination of between 4,500 and 4,710 lux. The average specific growth rate was calculated by dividing the natural log of the change in cells/ml between selected assays by the elapsed time (in hours) between the assays. The percentage inhibition in average cell growth rate was calculated as the difference between the control and each chemical test concentration. After the exposure of test substance with freshwater algae Selenastrum capricornutum, effects on biomass and growth rate were observed. Based on the biomass inhibition, EC50 was determine to be at 115 mg/l and on the basis of growth rate inhibition the EC50 was determine to be at 72.9 mg/l. The NOEC was observed at 10 mg/l. As the test chemical degrades rapidly in water, thus on the basis of readily biodegradability criteria, test chemical consider to be nontoxic and not classified as per the CLP classification criteria. 

 

Hence, on the basis of effects observations of test chemical on invertebrates and algae, it was concluded that the test chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.