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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No GLP statement or analysis
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Principles of method if other than guideline:
The acute toxicity of sodium chlorite to Daphnia magna in a 48 hr static test was assessed.
GLP compliance:
no
Analytical monitoring:
no
Details on sampling:
Water quality parameters were tested, namely temperature, hardness, dissolved oxygen and pH at 0 hr and 48 hr for the control and highest concentration.
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
19.6 °C
pH:
7.91 - 8.45
Dissolved oxygen:
6.7 - 9.3 mg/L
Nominal and measured concentrations:
Nominal concentrations:1, 10 and 100 mg/L (expressed as active component)(Doses are equivalent to 0.75, 7.5 and 75 mg chlorite/L)
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
< 1 other: mg sodium chlorite/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
< 0.75 other: mg chlorite/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Table A7_4_1_2 (3)-6:       Cumulative immobilisation data

Test Substance

Concentration
(nominal) 1

[mg sodium chlorite/L]

Immobile Daphnia

Number

    24 h          48 h    

Percentage

     24 h         48 h

0

0/5

0/5

0

0

0/5

0/5

0

0

0/5

0/5

0

0

0/5/

0/5

0

0

1

4/5

5/5

80

100

4/5

5/5

80

100

1/5

5/5

20

100

3/5

5/5

60

100

10

2/5

5/5

40

100

4/5

5/5

80

100

3/5

5/5

60

100

3/5

5/5

60

100

100

5/5

5/5

100

100

5/5

5/5

100

100

5/5

5/5

100

100

5/5

5/5

100

100

1 Concentrations were nominal

Table A7_4_1_2 (3)-7:       Effect data

EC501

95 % c.l.

EC01

EC1001

24 h [mg sodium chlorite/L]

Not specified

Not specified

Not specified

Not specified

48 h [mg sodium chlorite/L]

1 mg/L (n)

Not specified

Not specified

7 mg/L

1 effect data are based on nominal (n) concentrations

Validity criteria fulfilled:
yes
Remarks:
(immobilisation in the control was 0%; the dissolved oxygen concentration at the end of the test was greater than 3 mg/l in control and test vessels).
Conclusions:
The 48 hr EC50 < 1 mg sodium chlorite/L (equivalent to 0.75 mg chlorite/L).
Executive summary:

The aim of this study was to investigate the degree of inhibition of the Daphnia magna´s mobility produced by the test material.

The test procedure used was the OECD Guideline 202

Nominal concentrations: 1, 10 and 100 mg/L (expressed as active component) (Doses are equivalent to 0.75, 7.5 and 75 mg chlorite/L)

The result was as follows:

The 48 hr EC50 < 1 mg sodium chlorite/L (equivalent to 0.75 mg chlorite/L).

Description of key information

Key study. The test procedure used was the OECD Guideline 202 The 48 hr EC50 < 1 mg sodium chlorite/L (equivalent to 0.75 mg chlorite/L).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1 mg/L

Additional information

Key study. The test procedure used was the OECD Guideline 202

The 48 hr EC50 <1 mg sodium chlorite/L (equivalent to 0.75 mg chlorite/L).