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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The test substance could not be monitored in the test solutions due to background interference. However, the method allowed accurate determination of the stock solution concentrations in deionised water. As a flow-through system was used, the nominals are considered acceptable estimates of the actual concentration.
Qualifier:
according to
Guideline:
EPA OPP 72-1 (Fish Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Sampling:Dissolved oxygen, pH, salinity, and temperature were measured daily in each test chamber that contained live animals. The test temperature in one test vessel was recorded continuously during the test.
Monitoring of TS concentration:No, the test substance was not monitored throughout the test as background interference prevented it.
Vehicle:
not specified
Test organisms (species):
Cyprinodon variegatus
Test type:
flow-through
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
22.7 - 23 °C
pH:
7.6 - 8.2
Dissolved oxygen:
7.0 - 7.8 mg/L
Nominal and measured concentrations:
Nominal concentrations of the active ingredient were 0 mg/L (control), 22.5, 37.5, 60, 90 and 150 mg sodium chlorite/L. Test concentrations were adjusted for the purity of the test substance.(These doses are equivalent to 0, 17, 28, 45, 67 and 112 mg chlorite/L)
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
60 other: mg sodium chlorite/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
105 other: mg sodium chlorite/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95 % confidence interval of 90 to 150 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
78 other: mg clorite/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
60 other: mg sodium chlorite/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
45 other: mg chlorite/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
At a nominal concentration of 150 mg/L some fish were lethargic at 48 h. Exposure of Sheepshead minnows to the test substance resulted in a 96 h LC50 of 105 mg/L sodium chlorite with a 95 % confidence interval of 90 to 150 mg/L (equivalent to 78 mg chlorite/L). The 96 h NOEC was 60 mg/L sodium chlorite (equivalent to 45 mg chlorite/L).

Table A7_4_1_1(2)-5:        Mortality data

Test-Substance

Concentration
(nominal) [mg sodium chlorite/L]

Mortality

Number

    24 h          48 h          72 h          96 h

Percentage

    24 h         48 h          72 h           96 h

0 (control)

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

22.5

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

32.7

0

0

0

1

0

0

0

5

0

0

0

0

0

0

0

0

60

0

0

0

1

0

0

0

5

0

0

0

0

0

0

0

0

90

0

0

1

2

0

0

5

10

0

1

1

2

0

5

5

10

150

0

9

10

10

0

90

100

100

0

8

10

10

0

80

100

100

Table A7_4_1_1(2)-6:        Sub-lethal effect data

Test-Substance

Concentration
(nominal) [mg sodium chlorite/L]

Lethargy

Number

    24 h          48 h          72 h          96 h

Percentage

    24 h         48 h          72 h           96 h

0 (control)

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

22.5

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

32.7

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

60

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

90

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

150

0

1

-

-

0

5

-

-

0

2

-

-

0

10

-

-

Table A7_4_1_1(2)-7:        Effect data

96 h [mg/l]1

95 % c. l. [mg/l]

LC0

60

Not specified

LC50

105 (n)

90 to 150

LC100

Not specified

Not specified

1 indicate effect data are based on nominal (n) concentrations

Validity criteria fulfilled:
yes
Remarks:
(mortality in control was 0%; constant conditions were maintained; the dissolved oxygen concentration was within the acceptable range; the concentrations were maintained using a flow-through system).
Conclusions:
96 h LC50 = 105 mg/L sodium chlorite with a 95 % confidence interval of 90 to 150 mg/L (equivalent to 78 mg chlorite/L).
Executive summary:

The aim of this study was to investigate the short-term toxicity to fish produced by the test material.

The test procedure used was the EPA OPP 72-1 (Fish Acute Toxicity Test).

The result was as follows:

96 h LC50 = 105 mg/L sodium chlorite with a 95 % confidence interval of 90 to 150 mg/L (equivalent to 78 mg chlorite/L).

Description of key information

Key study. The test procedure used was the EPA OPP 72-1 (Fish Acute Toxicity Test).

The result was as follows:

96 h LC50 = 105 mg/L sodium chlorite with a 95 % confidence interval of 90 to 150 mg/L (equivalent to 78 mg chlorite/L).

Key value for chemical safety assessment

LC50 for marine water fish:
105 mg/L

Additional information

Key study.

The test procedure used was the EPA OPP 72-1 (Fish Acute Toxicity Test).

The result was as follows:

96 h LC50 = 105 mg/L sodium chlorite with a 95 % confidence interval of 90 to 150 mg/L (equivalent to 78 mg chlorite/L).