Paraffin oils, sulfochlorinated, saponified

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-03-20 till 1991-04-11

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

batch no.: 210291

Analytical monitoring:

yes

Details on sampling:

SAMPLE COLLECTION
The samples were collected by staff members of the ecotoxicology group. The samples were transferred into amber bottles and stored until analysis at below -20°C.

HANDLING
Analyses were performed in duplicate, no sample was stored.

Vehicle:

no

Details on test solutions:

- direct weighing
- Reconstituted water:
Hardness: 231.4 mg/L CaCO3
pH: 7.6
Dissolved oxygen: 100 %
9.3 mg/L
Temperature: 19.4 °C
- Solid test material:
The stock solution was prepared once before starting the test. The amount of test material used was 50.3 mg. The test material was dissolved in 0.5 Lreconstituted water and stirred for 10 min. The concentration of the stock solution was 100.6 mg/L.
Nominal concentration of the stock solution: 100.6 mg/L
pH-value: 8.2
Storage conditions:
Temperature: 21 ± 1 °C
Duration: 30 min in light

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna (Straus)
Supplier's address: Hoechst AG, Frankfurt/M.
Date of purchase: 1989-10-19
Size of stock vessel: 3.5 L; height: 10 cm; diameter: 22.5 cm

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

13.0 °dH (213.4 mg/L CaCO3)

Test temperature:

20.8 - 21.2 °C

pH:

7.9 -8.0

Dissolved oxygen:

8.6 - 9.3 mg/L

Nominal and measured concentrations:

Nominal concentration of the stock solution: 100.6 mg/L

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

9.48 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Test concentration was analytically confirmed

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.72 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Test concentration was analytically confirmed

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Test concentration was analytically confirmed

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.25 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Based on measured concentration at the end of the test

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.55 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Based on measured concentration at the end of the test

Details on results:

Highest test concentration causing no immobilization within the period of the range-finding test: 0.1 mg/L.
Lowest test concentration causing 100 % immobilization within the period of the range-finding test: 10.0 mg/L.

Reported statistics and error estimates:

Probit analysis was used to determine the EC values (FINNEY 1971). When less than 3 test substance concentrations caused immobilisation between 0% and 100% the geometrical mean was used to determine the EC50.

MBAS Content of the Product

Nominal Amount of Emulgator E 30 fest	Extinction E650	µg MBAS determined
2000 µg	n.d.	n.d.
1000 µg	3.5	682.1
500 µg	1.025	199.8
200 µg	0.661	128.8

n.d. = not determinable because the values were out of range

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of Paraffin oils, sulfochlorinated, saponified to aquatic invertebrates (Daphnia magna) was tested according to OECD guideline 202. During a period of 48 hours, an EC50 value of 3.25 mg/L was measured and the No Observed Effect Concentration (NOEC) of 0.55 mg/L was calculated (Leuna AG, 1992) .

Executive summary:

In accordance with GLP regulations acute toxicity of Emulgator E 30 to Daphnia magna (Straus), water-flea, was investigated in a static test system during a test period of 48 hours. The study was performed according to the OECD Guideline 202, Part I, 1984.

The nominal concentrations to which the test organisms were exposed were 0.1, 0.2, 0.4, 0.8, 1.6, 3.2 and 6.4 mg/L Emulgator E 30.

The analytically determined actual concentrations of the test material at the beginning of the study were found to be within the range of 43% and 156% with an average of 110.5%.

The analytically determined stability of the test material after 48 hours of exposure was found to be within the range of 14% and 128% of the nominal values with an average of 68.8%.

Analytical data were affected by "MBAS" of washing agents and the biodegradibility of the test material.

Based on nominal concentrations the study resulted in the following values:

EC50 (24 h) : 9.48 mg/L Emulgator E 30

EC50 (48 h) : 4.72 mg/L Emulgator E 30

No Observed Effect Concentration (NOEC): 0.8 mg/L Emulgator E 30

Based on actual (measured) concentrations at the end of the test (on average 68.8% nominal) the study resulted in the following values:

EC50 (48 h) : 3.25 mg/L Emulgator E 30

No Observed Effect Concentration (NOEC): 0.55 mg/L Emulgator E 30

Description of key information

The acute toxicity of Paraffin oils, sulfochlorinated, saponified to aquatic invertebrates (Daphnia magna) was tested according to OECD guideline 202. During a period of 48 hours, an EC50 value of 3.25 mg/L was measured and the No Observes Effect Concentration (NOEC) of 0.55 mg/L was calculated (Leuna AG, 1992) .

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.25 mg/L