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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Inhalation Risk Test conducted according to a BASF internal testing method. Acceptable, well documented report which meets basic scientific principles.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1969
Report Date:
1969
Reference Type:
other: Translation of the original German study report
Title:
Unnamed
Year:
2002
Report Date:
2001

Materials and methods

Principles of method if other than guideline:
BASF TEST:
The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temperature chosen for vapour generation (room temperature). 3 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 1, 3 and 8 h.
GLP compliance:
no
Remarks:
GLP was not compulsory at the time the study was conducted
Test type:
other: Inhalation Risk test
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Diaethylaethanolamin
- Analytical purity: 99 %

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 177 g (mean)

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Analytical verification of test atmosphere concentrations:
no
Remarks on duration:
1, 3 and 8 h
Concentrations:
The mean nominal concentration was about 7.7 mg/L (no analytical verification). The mean nominal concentration was in good agreement with the saturated vapour concentration estimated from the vapour pressure and thus could be used for further evaluations.
No. of animals per sex per dose:
3
Control animals:
no
Details on study design:
- Duration of observation period following administration: 8 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
other: Mortality
Effect level:
7.7 mg/L air
Based on:
test mat.
Exp. duration:
1 h
Remarks on result:
other: 0/12
Sex:
male/female
Dose descriptor:
other: Mortality
Effect level:
7.7 mg/L air
Based on:
test mat.
Exp. duration:
3 h
Remarks on result:
other: 5/6
Sex:
male/female
Dose descriptor:
other: Mortality
Effect level:
7.7 mg/L air
Based on:
test mat.
Exp. duration:
8 h
Remarks on result:
other: 5/6
Sex:
male/female
Dose descriptor:
LC50
Effect level:
ca. 4.6 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: Worst case graphical extrapolation on a probability sheet using the lethality rates after 1 and 3 hours was used for estimation of a LT50 of about 2.4 hours from which a 4-hour LC50 was calculated using Haber's rule.
Clinical signs:
Immediately escape attempts, gasping, dyspnoea and severe signs of irritation, namely corroded snouts, eyes, ears and front paws.
Gross pathology:
Corrosion of snouts, eyes, ears and front paws was noticed. 2 animals showed increased tracheal secretion and 1 animal, increased bronchial secretion.

Any other information on results incl. tables

Since the vapor pressure of the substance at 20°C is 1.9 mbar, the saturated vapor concentration is about 9.2 mg/l. Worst case graphical extrapolation from a probability sheet using the lethality rates after 1 and 3 hours of exposure results in an estimated LT50 of about 2.4 hours. From this a 4 hour LC50 of ca. 4.6 mg/l (4600 mg/m3) can be calculated using Haber's rule.

Applicant's summary and conclusion