2-diethylaminoethanol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Inhalation Risk Test conducted according to a BASF internal testing method. Acceptable, well documented report which meets basic scientific principles.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Reference 2

Reference Type:

other: Translation of the original German study report

Title:

Unnamed

Year:

2002

Report date:

2001

Materials and methods

Principles of method if other than guideline:

BASF TEST:
The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temperature chosen for vapour generation (room temperature). 3 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 1, 3 and 8 h.

GLP compliance:

no

Remarks:

GLP was not compulsory at the time the study was conducted

Test type:

other: Inhalation Risk test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-diethylaminoethanol

EC Number:

202-845-2

EC Name:

2-diethylaminoethanol

Cas Number:

100-37-8

Molecular formula:

C6H15NO

IUPAC Name:

2-(diethylamino)ethanol

Details on test material:

- Name of test material (as cited in study report): Diaethylaethanolamin
- Analytical purity: 99 %

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 177 g (mean)

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Analytical verification of test atmosphere concentrations:

no

Remarks on duration:

1, 3 and 8 h

Concentrations:

The mean nominal concentration was about 7.7 mg/L (no analytical verification). The mean nominal concentration was in good agreement with the saturated vapour concentration estimated from the vapour pressure and thus could be used for further evaluations.

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 8 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

other: Mortality

Effect level:

7.7 mg/L air

Based on:

test mat.

Exp. duration:

1 h

Remarks on result:

other: 0/12

Effect levels 2

Sex:

male/female

Dose descriptor:

other: Mortality

Effect level:

7.7 mg/L air

Based on:

test mat.

Exp. duration:

3 h

Remarks on result:

other: 5/6

Effect levels 3

Sex:

male/female

Dose descriptor:

other: Mortality

Effect level:

7.7 mg/L air

Based on:

test mat.

Exp. duration:

8 h

Remarks on result:

other: 5/6

Effect levels 4

Sex:

male/female

Dose descriptor:

LC50

Effect level:

ca. 4.6 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: Worst case graphical extrapolation on a probability sheet using the lethality rates after 1 and 3 hours was used for estimation of a LT50 of about 2.4 hours from which a 4-hour LC50 was calculated using Haber's rule.

Clinical signs:

other: Immediately escape attempts, gasping, dyspnoea and severe signs of irritation, namely corroded snouts, eyes, ears and front paws.

Gross pathology:

Corrosion of snouts, eyes, ears and front paws was noticed. 2 animals showed increased tracheal secretion and 1 animal, increased bronchial secretion.

Any other information on results incl. tables

Since the vapor pressure of the substance at 20°C is 1.9 mbar, the saturated vapor concentration is about 9.2 mg/l. Worst case graphical extrapolation from a probability sheet using the lethality rates after 1 and 3 hours of exposure results in an estimated LT50 of about 2.4 hours. From this a 4 hour LC50 of ca. 4.6 mg/l (4600 mg/m3) can be calculated using Haber's rule.