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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17. Dec 1968 - 27. Dec 1968
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically valid study, but not according to current standards or GLP.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1969
Report Date:
1969
Reference Type:
other: Translation of the original German study report XVIII/320
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Principles of method if other than guideline:
BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 401. A test group consisting of 10 animals/sex was treated by single gavage application with an aqueous solution of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy.


GLP compliance:
no
Remarks:
GLP was not compulsory at the time the study was conducted
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Diaethylaethanolamin
- Analytical purity: 99 %

Test animals

Species:
rat
Strain:
other: US-rats, not further specified
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: male: 204 g (mean); female: 167 g (mean)

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Doses:
177, 708, 1106 and 1416 mg/kg bw (the original values were reported as 200, 800, 1250 and 1600 µL/kg bw and were converted using a density d=0.885 g/cm3)
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 1 320 mg/kg bw
Remarks on result:
other: conversation in mg/kg bw based on the density: d=0.885 g/cm3
Mortality:
1416 mg/kg bw: 4 males and 9 females died within the first 24 hours; by 48 h one additional male died;
708 mg/kg bw: 1 male died on the 3rd day after application.
Clinical signs:
708 mg/kg: immediately after application apathy and dyspnea; by the next day the animals were mostly without symptoms;

1106 mg/kg: immediately after application apathy and dyspnea; on the next day dyspnea and unkempt fur;

1416 mg/kg: immediately after application apathy, unkempt fur and dyspnea; on the next day dyspnea and unkempt fur; the same symptoms were observed on the next day;
Body weight:
no data
Gross pathology:
Hemorrhage into the gastro-intestinal tract was observed in the animals that died.
The animals that survivied to the end had no unusual findings except for chronic bronchitis in 2 animals of the 708 mg/kg bw dose group.

Any other information on results incl. tables

Mortality:

 Dose (mg/kg bw)  Gender  Conc. %  1 h  24 h  48 h  day 7              
1416  male 20 0 4 5 5            
1416  female 20 0 9 9 9            
1106  male 10 0 0 0 0            
1106  female 10 0 0 0 0            
708  male 8 0 0 0 1            
708  female 8 0 0 0 0            
177  male 2 0 0 0 0            
177 female  2 0 0 0 0            

Applicant's summary and conclusion