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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Administrative data

Endpoint:
biodegradation in water: sediment simulation testing
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Acceptable study, meets basic scientific principles Hypothesis and analogue approach for read-across: This substance (S-Ethanol, composition 2) has degree of ethanol purity between 76 - 82%. Methanol is the main impurity of the target substance (conc. 13-14 %), and considered the major driver for adverse effects based on its properties and relative quantity in the substance. Therefore, testing of the substance itself was considered unnecessary. Instead the available REACH registration data of ethanol and methanol is used in order to update the chemical safety assessment of this substance. Other impurities of this substance are taken into account for self-classification, but there is no need to consider their properties in hazard assessment because of low concentrations (see IUCLID section 1.2; composition 2).

Data source

Reference
Reference Type:
publication
Title:
Methane production and simultaneous sulphate reduction in anoxic, salt marsh sediments
Author:
Oremland, R.S. et al.
Year:
1982
Bibliographic source:
Nature 296: 143-145

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Degradation test with methanol in anaerobic sediments from a salt marsh
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Methanol
- Analytical purity: no data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data
Radiolabelling:
yes

Study design

Oxygen conditions:
anaerobic
Inoculum or test system:
natural water / sediment
Details on source and properties of surface water:
- Details on collection (e.g. location, sampling depth, contamination history, procedure): San Francisco Bay Water
- salinity: ca. 2%
- sulphate: ca. 18mM
Details on source and properties of sediment:
- Details on collection (e.g. location, sampling depth, contamination history, procedure): upper 15 cm of a salt marsh, San Francisco Bay at Palo Alto, California, USA
- sediment: black, highly reduced, containing methane (ca. 8 mL/L wet sediment), strong smell of hydrogen sulphide. Sediment also contained abundant plant material (such as roots, rhizomes, stems and leaves) of Spartina foliosa
- Storage length: < 1h
- sampling: glass jars were filled completely with sediment to exclude air, sealed and transported back to the laboratory
Details on inoculum:
- Preparation of inoculum for exposure: Sediments were homogenized anaerobically with San Francisco Bay water. Homogenate was dispensed into Erlenmeyer flasks and sealed. The homogenate was further diluted with bay water to yield a slurry
Duration of test (contact time):
5 d
Initial test substance concentrationopen allclose all
Initial conc.:
310 other: µmol / 110 mL in flask
Based on:
test mat.
Initial conc.:
340 other: µmol / 110 mL in flask
Based on:
test mat.
Initial conc.:
500 other: µmol / 110 mL in flask
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: conversion of methanol to methane
Details on study design:
TEST CONDITIONS
- Test temperature: 19°C
- Continuous darkness: yes

TEST SYSTEM
- Method used to create anaerobic conditions: acc. to Oremland (1981)
- Number of culture flasks/concentration: 8 (4 with and without BES)
Reference substance
Reference substance:
not specified

Results and discussion

Test performance:
no details available
% Degradation
% Degr.:
83 - 91
Parameter:
other: conversion to methane
Sampling time:
3 d
Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 200-812-7]
IUPAC name:
methane
CAS number:
74-82-8
No.:
#2
Reference
Reference substance name:
Unnamed
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 204-696-9]
IUPAC name:
dioxomethane
CAS number:
124-38-9
No.:
#3
Reference
Reference substance name:
Unnamed
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 231-791-2]
IUPAC name:
water
Identity:
Distilled water
Identity:
Hydrogen oxide (H2O)
Identity:
Water
CAS number:
7732-18-5
Molecular formula:
H2O
Molecular weight:
18.015
SMILES notation:
O
InChl:
InChI=1/H2O/h1H2
Details on transformation products:
no further details
Evaporation of parent compound:
not specified
Volatile metabolites:
not specified
Residues:
not specified
Details on results:
no further details
Results with reference substance:
no data

Any other information on results incl. tables

In the experiments with dilute slurries, methanol-amended flasks produced methane and production levelled off after 5 days incubation. After 3 days incubation, 83-91% conversion of the methanol had occurred.

Methanol stimulates methanogenesis but not sulphur reduction.

Applicant's summary and conclusion