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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge
Test temperature:
25 °C (column temperature)
Details on study design: HPLC method:
EQUIPMENT
- Apparatus:
- Type: Agilent 1200 Series Liquid Chromatograph
- Type, material and dimension of analytical (guard) column: HyperClone CN (CPS) (25 cm x 4.6 mm internal diameter)
- Detection system: UV set at 210 nm

MOBILE PHASES
- Type: Acetonitrile:tetrahydrofuran:buffer solution (30:25:45 v/v/v), where a phosphate buffer solution was employed for the pH 4 test and a borate buffer solution for the pH 9 test
- Experiments with additives carried out on separate columns: no additives used
- pH: 4 and 9
- Solutes for dissolving test and reference substances: HPLC mobile phase

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column (formamide)

REFERENCE SUBSTANCES
- Identity: Acetanilide, Nitrobenzene, Naphthalene, 2-Methylnaphthalene, Anthracene, DDT
- Known logKoc values: 1.3, 2.4, 2.8, 3.9, 4.4, 5.6

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 20 µg
- Quantity of reference substances:
Formamide (internal deadtime standard): 4.1 µg, Acetanilide: 0.2 µg, Nitrobenzene: 0.2 µg, Naphthalene: 0.1 µg, 2-Methylnaphthalene: 0.1 µg, Anthracene: 0.2 µg, DDT: 0.4 µg
- Intervals of calibration: before and after injections of the test substance

REPETITIONS
- Number of determinations: two

EVALUATION
- Determination of the log Koc value: Since the retention times of the test material were outside those of the standards limit values are reported.
Key result
Type:
log Koc
Value:
> 5.6
pH:
4
Temp.:
25 °C
Key result
Type:
log Koc
Value:
< 1.3
pH:
9
Temp.:
25 °C
Details on results (HPLC method):
- pH 4 test: All major components of the test material eluted after DDT, indicating a log10Koc values of >5.6.

- pH 9 test: Most major components of the test material eluted before acetanilide, the first eluting standard at 3.3 minutes, indicating a log10Koc value of < 1.3, although some minor components eluted later.

Description of key information

Log Koc at 25 °C: > 5.6 and < 1.3 at pH 4 and 9, respectively (HPLC method; OECD 121, EU C.19)

Key value for chemical safety assessment

Additional information