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Table 6.1.1 Summary of aquatic toxicity test results

Test species

Test protocol

Year test completed

Endpoint and exposure period

Result (mg/l)1

Reliability assessment

Comments

Reference

Toxicity to fish

Rainbow trout (Oncorhynchus mykiss)

OECD 203

2002

96-h NOEC

24-h LC50

48-h LC50

72-h LC50

96-h LC50

38

>92

40-70

53

52

(1) valid without restriction

Fulfils all the reliability criteria. Semi-static test. The test was subject to GLP

L'Haridon J, 2002

Rainbow trout (Oncorhynchus mykiss)

OECD 203

1993

96-h NOEC

24-h LC50

48-h LC50

72-h LC50

96-h LC50

>10 (N)

>10 (N)

>10 (N)

>10 (N)

>10 (N)

(2) valid with restrictions

Static limit test. The test was not supported by analysis of exposure concentrations or GLP

Sewell IG et al, 1993

Fish - acute QSAR (Esters)

ECOSAR (version 0.99g)

96-h LC50

32

(2) valid with restrictions

Reliable calculation method

US EPA, 2000

Fish – acute QSAR (Phosphate esters)

ECOSAR (version 0.99g)

96-h LC50

17

(2) valid with restrictions

Reliable calculation method

US EPA, 2000

Fish – chronic QSAR (Esters)

ECOSAR (version 0.99g)

NOEC

7.0

(2) valid with restrictions

Reliable calculation method

US EPA, 2000

Toxicity to aquatic invertebrates

Cladoceran (Daphnia magna)

OECD 202

2002

48-h NOEC

24-h EC50

48-h EC50

21

84

42

(1) valid without restriction

Fulfils all the reliability criteria. The test was subject to GLP.

L'Haridon J, 2002

Cladoceran (Daphnia magna)

OECD 202

1993

48-h NOEC

24-h EC50

48-h EC50

>10 (N)

>10 (N)

>10 (N)

(2) valid with restrictions

Static limit test. The test was not supported by analysis of exposure concentrations or GLP

Sewell IG et al, 1993

Invertebrate - acute QSAR(Esters)

ECOSAR (version 0.99g)

48-h LC50

81

 (2) valid with restrictions

Reliable calculation method  

US EPA 2000

Cladoceran (Daphnia magna)

OECD 211

2002

23 day EC50(parent mortality)

23-day NOEC reproduction

7.31

>3.68

(1) valid without restriction

Fulfils all the reliability criteria. Test duration extended to 23 days in order to achieve validity criteria for control reproduction. The study was subject to GLP

L'Haridon J, 2003

Invertebrate – longer term repro QSAR (Neutral organics)

ECOSAR (version 0.99h)

16-d EC50(reproduction)

6.0

  (2) valid with restrictions

Reliable calculation method

US EPA, 2000

Toxicity to algae

Freshwater alga (Pseudokirchneriella subcapitata)

OECD 201

2002

72-h NOEC

72-h ErC50(growth rate)

72-h EbC50(biomass)

10

35     

21

(1) valid without restriction

Fulfils all the reliability criteria. The test was subject to GLP

L'Haridon J, 2002

Freshwater alga (Scenedesmus subspicatus)

OECD 201 (Limit test)

1994

72-h NOEC

72-h ErC50(growth rate)

72-h EbC50(biomass)

>10 (N)

>10 (N)

>10 (N)

(2) valid with restrictions

Limit test. The test was not supported by analysis of exposure concentrations or GLP

Sewell IG et al, 1993

Algae QSAR (Esters)

ECOSAR (version 0.99g)

96-h EC50

96-h NOEC

2.6

2.1

 (2) valid with restrictions

Reliable calculation method

US EPA, 2000

Toxicity to micro-organisms

Activated Sludge

OECD 209

1993

3h EC50

> 1000

(2) valid with restrictions

The test was not subject to GLP

Sewell IG et al, 1993

1. 'N' denotes result expressed as nominal concentration