Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Chemical name: 2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate)
Product name: Amgard V6

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
A mixed population of activated sludge micro-organisms obtained from the aeration stage of the Severn Trent Water plc sewage treatment plant at Belper, Derbyshire, UK, treating predominantly domestic sewage
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 other: mg C/L
Based on:
DOC
Initial conc.:
79.7 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Test temperature: 21-22 degree C
- pH: 7.4 (test material and control) 7.5 (standard material)
- Continuous darkness: yes

TEST SYSTEM
- Measuring equipment: Ionic 555 TOC Analyzer

SAMPLING
- Sampling frequency: samples taken on day 1, 2, 3, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 27, 28 and 29 (first vessel), 0, 29 (second vessle)
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
5
Sampling time:
29 d
Details on results:
Assessment of ready biodegradability using the CO2 evolution test (modified Sturm test) - according to OECD guidelines, the chemical could not be considered as ready biodegradable (see table 1). Additional investigational work using the activated sludge respiration inhibition test (OECD guideline no.209) confirmed that the test material did not exhibit any toxic effects on the inoculum at the concentrations employed in the test.

BOD5 / COD results

Results with reference substance:
Sodium benzoate: 97% degradation after 28 days, pass value reached within 10 days. Suitability of test method demonstrated.

Any other information on results incl. tables

Table 5.2.1.1: Cummulative biodegradation (%) of the test and reference substance

% cummulative biodegradation

at sampling time (days)

1

2

3

6

8

10

18

24

29

Test substance

0

0

0

0

0

1

0

0

5

Reference substance

3

41

54

79

77

82

88

89

97

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
reference substance (sodium benzoate): 97% degradation after 28 days, pass level reached within 10 days
Interpretation of results:
other: not readily biodegradable
Conclusions:
2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate) is not ready biodegradable according to the OECD guidelines (5% degradation after 28 days)
Executive summary:

In a screening test for biodegradation in water (OECD Guideline No 301B) 2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl)phosphate) attained 5% degradation after 28 days. The test substance is therefore considered to be not readily biodegradable, according to terms and conditions of the OECD Guidelines.

An activated sludge respiration inhibition test (OECD guideline no.209) confirmed that the test material did not exhibit any toxic effects on the inoculum at the concentrations employed in the test.