Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
acute, 48 hours, 15 to 17 June 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline and GLP-compliant study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Principles of method if other than guideline:
Similar to EPA OPPTS 850.1010.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dimethylolpropionsaure (reported as Bis-MPA in this summary)
- Physical state: white solid
- Analytical purity: 99.4%
- Lot/batch No.: SE 8069
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples were taken at 0 hours (fresh media) and 48 hours (expired media) from the control, 12.5 and 00 mg/L test treatments.

Test solutions

Vehicle:
no
Details on test solutions:
The test material was stirred in M4 medium for about 10 minutes at 20 ± 2 °C. The nominal concentration of the stock solution was 100 mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus 1820
- Source: Institut National de Recherche Chimique Appliqueé, France
- Age at study initiation (mean and range, SD): 2 - 24h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Not applicable

Test conditions

Hardness:
2.2 - 3.2 mmol/L
Test temperature:
19.9 - 20.3°C
pH:
See tables in results section
Dissolved oxygen:
See tables in the results section.
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal: 0 (control) 12.5, 25, 50, 100 mg/L; neutralised: 50, 100 mg/L
Measured: <5 (control) , 12.5, 102 mg/L in the control, 12.5 and 100 mg/L treatments.
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
See tabulated data below.
Results with reference substance (positive control):
The ECso(24h) of the control substance potassium dichromate was 1,30 mg/L. (Date of the last control experiment: 25 May 1999).
Reported statistics and error estimates:
Not applicable

Any other information on results incl. tables

Analytical results are presented below:

 

Nominal Bis-MPA concentration (mg/L)

Measured concentrations (mg/L)
(each value is a mean of two determinations)

0 hour

48 hours

Mean

% of nominal

Control

<5

<5, <5

-

-

12.5

12.4

12.3, 12.9

12.5

100

100

102

102, 104

103

103

 

The immobility results are presented below:

Nominal Bis-MPA concentration (mg/L)

Number of immobilised Daphnia
(20 Daphnia per concentration)

24 hours

% immobility

48 hours

% immobility

Control

0

0

0

0

12.5

0

0

0

0

25

0

0

0

0

50

0

0

0

0

100

0

0

0

0

Neutralised test media

50

0

0

1

5

100

0

0

0

0

 

The water quality values are presented below:

Nominal Bis-MPA concentration (mg/L)

Parameter

pH

Dissolved oxygen (mg/L)

0 hours

48 hours

0 hours

48 hours

Control

8.0

8.0

8.4

8.8

12.5

7.9

8.0

8.5

8.7

25

7.7

8.0

8.5

8.6

50

7.3

7.7

8.3

8.6

100

5.8

7.6

8.3

8.6

Neutralised test media

50

7.8

7.9

8.3

8.5

100

7.5

7.8

8.2

8.5

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48 hour acute toxicity of Bis-MPA to Daphnia magna was carried out in a static system according to OECD 202 and EU guidelines. Four replicate vessels, each containing 5 daphnids were exposed to nominal concentrations of 0 (control), 12.5, 25, 50 and 100 mg/L over a 48 hour exposure period. The measured pH in the freshly prepared 50 and 100 mg/L test media were 7.3 and 5.8, therefore additional test vessels were prepared where the pH was neutralised. Measured concentrations demonstrated that test media were adequately prepared and maintained during the exposure period, the results of the study were therefore based on nominal exposure concentrations. pH had no effect on Daphnia mobility during the study. The 48-hour EC50 was determined to be >100 mg/L (the highest nominal concentration tested).
Executive summary:

The 48 hour acute toxicity of Bis-MPA to Daphnia magna was carried out in a static system according to OECD 202 and EU guidelines. Four replicate vessels, each containing 5 daphnids were exposed to nominal concentrations of 0 (control), 12.5, 25, 50 and 100 mg/L over a 48 hour exposure period. The measured pH in the freshly prepared 50 and 100 mg/L test media were 7.3 and 5.8, therefore additional test vessels were prepared where the pH was neutralised. Measured concentrations demonstrated that test media were adequately prepared and maintained during the exposure period, the results of the study were therefore based on nominal exposure concentrations. pH had no effect on Daphnia mobility during the study. The 48-hour EC50 was determined to be >100 mg/L (the highest nominal concentration tested)