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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, meets national standard methods, limited documentation, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: Norme Francais EN 28692
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyrene
EC Number:
204-927-3
EC Name:
Pyrene
Cas Number:
129-00-0
Molecular formula:
C16H10
IUPAC Name:
pyrene
Details on test material:
- Name of test material (as cited in study report): pyrene
- Molecular formula (if other than submission substance): C16H10
- Molecular weight (if other than submission substance): 202
- Substance type: organic
- Physical state: solid
- Source: no data
- Analytical purity: no data
- Typical concentration in coal-tar pitch: 0.94 - 1.48 % (data from NL 2008: Coal-Tar Pitch, Annex-XV do
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier, and WHO 1998: EHC 202, if not otherwise indicated)
- Vapour pressure: 0.6 - 1.0*10^-3 Pa (at 25 °C)
- Water solubility: in freshwater, approx. 125 - 135 µg/L (20 - 25 °C);
soluble portion of the WSF of coal-tar pitch (loading 100 mg/L): 158 - 326 ng/L (mean 240 ng/L, n = 5)
[see 4.8: NOACK 2009_WSF, 100 mg/L nominal]
- Henry's law constant: 1.4 Pa*m3/mol (at 25 °C)
- log Pow: = 5.0 - 5.2

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
at 0. 24, 48, and 72 h

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution close to water saturation used for dilution series (no detailed data, see Report p. 34)
- Eluate: no
- Differential loading: no
- Controls: with and without vehicle
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no data

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Source (laboratory, culture collection): in-house, origin Culture Centre of Algae and Protozoa, University of Cambridge
- Age of inoculum (at test initiation):
- Method of cultivation: weekly inoculation into fresh culture medium

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
approx. 215 mg/L (as CaCO3)
Test temperature:
23 +-2°C
pH:
no data
Nominal and measured concentrations:
nominal: not documented
analytical: Estimation of the EC values from measured concentrations at 0, 24, 48, and 72 h,
based on the degradation constant (see Report, p. 40 - 44).
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Erlenmeyer 250 mL, test volume 100 mL
- Aeration: no
- Initial cells density: approx. 10^4 cells/mL
- Control end cells density:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 3
- agitation: orbital shaker, 150 rpm


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic mediumprepared from stock solutions of mineral salts (Report., p. 29/30)

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 6000 - 8000 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter (Coulter Counter) at 24, 48, and 72 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: no data
- Range finding study no data
Reference substance (positive control):
yes
Remarks:
potassium bichromate K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
1.2 µg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: biomass and growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
12.4 µg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: biomass and growth rate
Remarks on result:
other: 95% C.I.: 6.7 - 17.9 µg/L
Results with reference substance (positive control):
- Results with reference substance valid? yes
- mean ECb50: 0.22 - 0.45 mg/L
- mean ECr50: 0.67 - 1.05 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Adverse effects within the range of water solubility