α,α-bis[4-(dimethylamino)phenyl]-4-(phenylamino)naphthalene-1-methanol

Endpoint summary

Description of key information

Adsorption / desorption

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. A test item solution was prepared by accurately weighing 20 mg of test item and diluted with ACN up to 10 ml. Thus, the test solution concentration was 2000 mg/l. The sample was sonicated for 2 minutes and filtered through a 0.22 µm syringe filter. The pH of the sample was found to be 7.9. Each of the reference substance and test substance were analysed by HPLC at 210 nm. For the HPLC method, HPLC model no. Agilent Technologies,1260 Infinity II was used. Column used was Waters sperisorb, 4.6*250 mm, 5µ (CL-25). Acetonitrile : water (55:45) was used as a mobile phase. During the study, flow rate was 1.0ml/min. Study was performed at a temperature of 25°C. Injector volume was 10μl. Chem Station Open lab Control Panel was used as a data Acquisition software and detector wavelength taken for the study was 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k' were calculated. The graph was plotted between log Koc versus log k'. The linear regression parameter of the relationship log Koc vs log k' were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to estimated Koc range of the test substance and generalized calibration graph was prepared. The reference substances Aniline, 4-Chloroaniline, 2,5-Dichloroaniline, Naphthalene, Biphenyl, Phenanthrene, Triphenylamine and DDT were chosen having log Koc range from 1.8 to 5.4. The Log Koc value of test chemical was determined to be 6.475±0.010 at 25°C.This log Koc value indicates that the test chemical has a very strong sorption to soil and sediment and therefore have negligible migration potential to ground water.

Additional information

Adsorption / desorption

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. A test item solution was prepared by accurately weighing 20 mg of test item and diluted with ACN up to 10 ml. Thus, the test solution concentration was 2000 mg/l. The sample was sonicated for 2 minutes and filtered through a 0.22 µm syringe filter. The pH of the sample was found to be 7.9. Each of the reference substance and test substance were analysed by HPLC at 210 nm. For the HPLC method, HPLC model no. Agilent Technologies,1260 Infinity II was used. Column used was Waters sperisorb, 4.6*250 mm, 5µ (CL-25). Acetonitrile : water (55:45) was used as a mobile phase. During the study, flow rate was 1.0ml/min. Study was performed at a temperature of 25°C. Injector volume was 10μl. Chem Station Open lab Control Panel was used as a data Acquisition software and detector wavelength taken for the study was 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k' were calculated. The graph was plotted between log Koc versus log k'. The linear regression parameter of the relationship log Koc vs log k' were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to estimated Koc range of the test substance and generalized calibration graph was prepared. The reference substances Aniline, 4-Chloroaniline, 2,5-Dichloroaniline, Naphthalene, Biphenyl, Phenanthrene, Triphenylamine and DDT were chosen having log Koc range from 1.8 to 5.4. The Log Koc value of test chemical was determined to be 6.475±0.010 at 25°C.This log Koc value indicates that the test chemical has a very strong sorption to soil and sediment and therefore have negligible migration potential to ground water.