2-Propanol, 1,3-bis[(3-methyl-2-buten-1-yl)oxy]-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2021-09-28 to 2021-10-27

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Adopted: 2004-04-13

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all test item concentrations and control (0 h and 48 h), retain samples were taken
- Sample storage conditions before analysis: Deep frozen

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 60 minutes stirring of stock solution at 100 mg/L
- Controls: Test medium control
- Concentration of final test solutions: 100, 50.0, 25.0, 12.5, 6.25 mg/L and control
- Test concentration separation factor: 2.0
- Evidence of undissolved material: Solutions were clear and transparent

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Strain, Daphnia magna (Crustacea, Cladocera) (Straus, 1820), clone: V
- Age at study initiation: young daphnia with age < 24 h
- Method of breeding: Daphnia magna was bred as single culture (1 daphnid per 100 mL) in test medium (a mix of dechlorinated drinking water and deionised water). The pH-value was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH. The animals were fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.
- Source: Federal Environment Agency in Berlin/Germany
- Feeding during test: None

Test type:

static

Water media type:

freshwater

Remarks:

Elendt M4 test medium

Limit test:

no

Total exposure duration:

48 h

Hardness:

14°dH (250 mg/L as CaCO3)

Test temperature:

19.7 – 20.4 °C

pH:

7.66 – 8.11

Dissolved oxygen:

> 7.87 mg/L

Nominal and measured concentrations:

Nominal: 100, 50.0, 25.0, 12.5, 6.25 mg/L
Measured (geometric mean): 132, 61, 26.4, 12.9, 7.23 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers
- Type: covered with a glass plate
- Fill volume: 100 mL
- Volume of solution: = 50 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4, one additional replicate for physico - chemical measurements without test organisms
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 test medium
- Culture medium different from test medium: yes (a mix of dechlorinated drinking water and deionised water used as culture medium)

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod /8 hours darkness daily
- Light intensity: 1107 lux (mean)

VEHICLE CONTROL PERFORMED: no

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

61 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

82.7 mg/L

95% CI:

71.1 - 96.1

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

66 mg/L

95% CI:

57.7 - 75.4

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: None
- Immobilisation of control: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: All solutions appeared to be clear and colourless.

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- ECx: between 0.6 and 2.1 mg/L (only these 2 concentrations tested)

Reported statistics and error estimates:

The 24 h and 48 h EC50 are the calculated concentrations where 50 % of the daphnids were immobilised after 24 and 48 hours, respectively.
Due to a lack of immobilisation, the EC50-value after 24 hours was not determined statistically.
The value for EC50 after 48 hours was determined by Spearman-Karber procedure.
The evaluation of data was performed by ToxRat Professional 3.3.0. ToxRat Professional, Version 3.3.0 performs calculations with unrounded values.
The NOEC was established based on the highest concentration at which the immobilisation is not higher than the allowed control immobilisation (= 10 % immobilisation).

Validity Criteria of the Study

Control immobilisation: The percentage of immobilisation should be ≤ 10 %. In this study the control immobilisation was 0 %.

Oxygen concentration: The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in all test units. In this test, the dissolved oxygen concentration at the end of the test was ≥ 7.87 mg/L.

Table 1:          Results of the test, 24 h values

	Nominal test item concentration [mg/L]
	Control	6.25	12.5	25.0	50.0	100
	Immobilised daphnids after 24 h
Replicate 1	0	0	0	0	0	1
Replicate 2	0	0	0	0	1	0
Replicate 3	0	0	0	0	0	0
Replicate 4	0	0	0	0	0	0
Σ	0	0	0	0	1	1
%	0	0	0	0	5	5

Table 2:          Results of the test, 48 h values

	Nominal test item concentration [mg/L]
	Control	6.25	12.5	25.0	50.0	100
	Immobilised daphnids after 48 h
Replicate 1	0	0	0	0	0	4
Replicate 2	0	0	0	1	1	5
Replicate 3	0	0	0	0	1	5
Replicate 4	0	0	0	0	0	5
Σ	0	0	0	1	2	19
%	0	0	0	5	10	95

Table 3:          Appearance of the test solution

Nominal test item concentration [mg/L]	0 h	24 h	48 h
Control	Clear and transparent	Clear and transparent	Clear and transparent
All treatment groups (100, 50.0, 25.0, 12.5 and 6.25 mg/L)	Clear and transparent	Clear and transparent	Clear and transparent

Table 4:          Temperature of the test solutions

	Nominal test item concentration [mg/L]
	Control	6.25	12.5	25.0	50.0	100
Time [h]	Temperature [°C]
0 fresh	19.7	19.9	19.9	19.9	20.0	20.1
24 aged	20.3	20.4	20.3	20.3	20.2	20.3
48 aged	20.0	19.9	19.9	19.8	19.9	19.8
Mean	20.0	20.1	20.0	20.0	20.0	20.1
Std. dev.	0.3	0.3	0.2	0.3	0.2	0.3
Mean	20.0
Std. dev.	0.2

Table 5:          pH-values of the test solutions

	Nominal test item concentration [mg/L]
	Control	6.25	12.5	25.0	50.0	100
Time [h]	pH
0 fresh	7.99	8.07	8.09	8.09	8.10	8.11
24 aged	7.90	7.94	7.93	7.93	7.91	7.94
48 aged	7.66	7.88	7.94	7.94	7.94	7.94
Mean	7.85	7.96	7.99	7.99	7.98	8.00
Std. dev.	0.17	0.10	0.09	0.09	0.10	0.10
Mean	7.96
Std. dev.	0.11

Table 6:          O₂ concentration of the test solutions

	Nominal test item concentration [mg/L]
	Control	6.25	12.5	25.0	50.0	100
Time [h]	Oxygen [mg/L]
0 fresh	9.00	8.99	8.96	8.96	8.96	8.97
24 aged	8.54	8.15	7.98	7.87	7.89	8.06
48 aged	8.25	8.31	8.40	8.15	8.31	8.27
Mean	8.60	8.48	8.45	8.33	8.39	8.43
Std. dev.	0.38	0.45	0.49	0.57	0.54	0.48
Mean	8.45
Std. dev.	0.42

Table 7:          Composition of Elendt M4 test medium

Stock solution	Concentration of stock solution		Amount (mL) of stock solution for 60 L final medium	Concentrations in final medium mg/L
Calcium chloride	CaCl2 × 2 H2O	588.16 g/L	30	294
Magnesium sulfate	MgSO4 × 7 H2O	246.6 g/L	30	123
Sodium hydrogencarbonate	NaHCO3	81.0 g/L	48	64.8
Potassium chloride	KCl	11.6 g/L	30	5.80
Cation solution	MnCl2 × 4 H2O	21.63 g/L	1	0.3605
	LiCl	18.36 g/L		0.306
	RbCl	4.26 g/L		0.071
	SrCl2  × 6 H2O	9.12 g/L		0.152
	CuCl2 × 2 H2O	710.8 mg/L		0.0118
	ZnCl2	780 mg/L		0.0130
	CoCl2 × 6 H2O	600 mg/L		0.0100
Anion solution	NaNO3	3.29 g/L	5	0.274
	H3BO3	34.31 g/L		2.86
	NaBr	0.192 g/L		0.0160
	Na2MoO4 × 2 H2O	0.738 g/L		0.0615
	KI	39 mg/L		0.00325
	Na2SeO3	26.3 mg/L		0.00219
	NH4VO3	6.9 mg/L		0.000575
Silica solution	Na2 SiO3 × 9 H2O	120 g/L	5	10.0
EDTA- Ferric sulphate solution	Titriplex III  × 2 H2O	5 g/L	30	2.50
	FeSO4 × 7 H2O	1.991 g/L		0.996
Phosphate solution	KH2PO4	8.58 g/L	1	0.143
	K2HPO4	11.04 g/L		0.184
Vitamine solution	Thiamindihydrochloride	4.5 g/L	1	0.0750
	Cyanocobalamine (B12)	60 mg/L		0.00100
	Biotine	45 mg/L		0.000750

Table 8:          Results of the toxic reference test, started on 28 September 2021

K2Cr2O7	24 h			48 h
[mg/L]	0.6	2.1		0.6		2.1
	immobilised daphnids
Replicate 1	0		5	0	5
Replicate 2	0		5	0	5
Replicate 3	0		5	0	5
Replicate 4	0		5	0	5
Σ	0		20	0	20
%	0		100	0	100

Validity criteria fulfilled:

yes

Conclusions:

According to the results of the test, the EC50 (48 h) for immobilisation was determined to be 66.0 mg/L (nominal) and 82.7 mg/L (mean measured). The NOEC (48 h) was set at 50.0 mg/L (nominal) and 61 mg/L (mean measured).

Description of key information

According to the results of the test, the EC50 (48 h) for immobilisation was determined to be 66.0 mg/L (nominal) and 82.7 mg/L (mean measured). The NOEC (48 h) was set at 50.0 mg/L (nominal) and 61 mg/L (mean measured).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

82.7 mg/L