[No public or meaningful name is available]

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

Source: SAG, Culture Collection of Algae at Pflanzenphysiologisches Institut of the University at Göttingen, Albrecht von Haller Institut, Untere Klarspüle 2, 37073 Göttingen, germany. Catalogue: no. 61.81

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

none

Test conditions

Hardness:

A sterilized synthetic growth medium (OECD medium) according to OECD 201 was used for culture and preparation of the test medium.

Test temperature:

21.5-22.0°C

pH:

7.45-8.03

Dissolved oxygen:

-

Salinity:

-

Nominal and measured concentrations:

Nominal: 6.25, 12.5, 25, 50 and 100 mg/l; measured: all < 0.35 mg/l

Details on test conditions:

test vessel: 250 ml conicalglass flasks
type of vessel: covered with silicone-sponge caps (sterilized prior to use)
Initial cells' density: 10,000 cells/ml
Number of organisms per vessel: 1,000,000
Number of vessels per concentration: 3 replicates
Number of vessels per control: 6 replicates

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for growth rate and yield was > 100 m/l based on nominal loading. The NOEC was determined to be >= 100 mg/l (nominally based). Basedon measured concentrations, the values EC50 values were > 0.35 mg/l, and the NOEC values were >= 0.35 mg/l.