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EC number: 258-420-7 | CAS number: 53185-52-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 October 2011 - 19 October 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Official Journal of the European Communities, Ll33 A, Part C, Activated sludge respiration inhibition test.
- Version / remarks:
- 1988
- Deviations:
- no
- GLP compliance:
- yes
- Vehicle:
- no
- Details on test solutions:
- Method of preparation: two stock solutions were prepared at a nominal rate of 5000 mg test item/L in reverse osmosis (RO) water, the day before use. These stocks were observed to be clear colourless solutions. The pH was was adjusted to 7.8 with hydrochloric acid. Test solutions were prepared by the addition of a known volume of this stock solution to a total volume of 500 mL RO water, synthetic sewage and activated sludge.
Control: containing activated sludge, synthetic sewage feed and dilution water only.
Abiotic control: containing reference substance, synthetic sewage feed, reference substance and dilution water only (no activated sludge). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Totnes Sewage Works, Totnes, Devon, UK
- Method of cultivation: activated sludge was obtained 1 day prior to exposure. The activated sludge was settled, the supernatant decanted. The settled sludge was fed with 50 mL of OECD synthetic sewage feed per litre of sludge per day and aerated at room temperature, until it was used in the test.
- Preparation of inoculum for exposure: a synthetic sewage mixture was prepared as described by OECD 209.
- Initial biomass concentration: 4373 mg of filterable solids/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +/- 2 °C
- pH:
- Adjusted to 7.8 at the start of the test.
- Nominal and measured concentrations:
- Nominal concentrations: 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: no detailed information on test vessels was provided; final flask fill volume: 500 mL
- Aeration: yes
- No. of vessels per concentration (replicates): one for 10 and 100 mg/L; three for 1000 mg/L
- No. of vessels per control (replicates): two
- No. of vessels per reference control (replicates): four (nominal concentrations of 3.2, 10, 32 and 100 mg/L)
- No. of vessels per abiotic control (replicates): one (100 mg/L)
- Sludge concentration (weight of dry solids per volume): 1500 mg/L
- Nutrients provided for bacteria: synthetic sewage feed
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: water treated with reverse osmosis
OTHER TEST CONDITIONS
- Adjustment of pH: yes, to 7.8 with hydrochloric acid
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Respiration rate after 3 hours (microcathode oxygen electrodes, connected to an interface unit) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - <10% inhibition was observed in all tested concentrations.
- Results with reference substance (positive control):
- - 3h-EC50: 9.1 mg/L
- Result was within the expected range and confirmed the sensitivity of the used sludge. - Validity criteria fulfilled:
- yes
- Remarks:
- Oxygen update in de controls was >20 mg O2/g activated sludge (e.g., >22 mg/g), the CV of oxygen uptake in the control was <30% (e.g., 9%) and the EC50 of the reference item was in the range of 2-25 mg/L (e.g., 9.1 mg/L)
- Conclusions:
- The 3h-EC50 for Equamide M100 based on inhibition of respiration was > 1000 mg/L. The NOEC was 1000 mg/L.
- Executive summary:
In an activated sludge respiration inhibition test , according to OECD 209, Equamide M100 was tested for its effect on activated domestic sludge. Nominal concentrations of 10, 100 and 1000 mg/L were used, together with a control. The reference substance, 3,5 -dichlorophenol was tested at concentrations of 3.2, 10, 32 and 100 mg/L and an abiotic control (containing no sludge) was included as well. Results showed that Equamide M100 did not inhibit respiration up to the highest tested concentration.
Reference
Study deviation: the control, abiotic control and reference substance flasks were aerated for 3.5 hours instead of 3 hours. This additional aeration did not compromise the data or invalidate the conclusion.
Table 1 Inhibition results
Test substance |
Nominal test concentration (mg/L) |
pH |
Respiration rate (mg/L/h) |
Percentage inhibitiona |
|
Start |
End |
||||
Control |
Control |
7.0 |
6.2 |
13.4 |
- |
3,5-DCPb |
100 (abiotic) |
7.9 |
7.8 |
-5.6 |
- |
3,5-DCP |
100 |
8.0 |
7.7 |
0.5 |
96.2 |
3,5-DCP |
32 |
7.1 |
7.8 |
3.1 |
78.4 |
3,5-DCP |
10 |
7.0 |
7.7 |
6.7 |
53.1 |
3,5-DCP |
3.2 |
7.0 |
7.7 |
12.2 |
14.9 |
Control |
Control |
6.9 |
6.2 |
15.3 |
- |
Equamide M100 |
1000 |
6.9 |
6.4 |
13.5 |
<10 |
Equamide M100 |
1000 |
6.9 |
6.4 |
16.1 |
<10 |
Equamide M100 |
1000 |
6.9 |
6.7 |
18.1 |
<10 |
Equamide M100 |
100 |
6.9 |
6.5 |
13.6 |
<10 |
Equamide M100 |
10 |
6.9 |
6.6 |
13.8 |
<10 |
a calculated using mean
control respiration rate of 14.4 mg/L/h
b 3,5-dichlorophenol
Description of key information
In an activated sludge respiration inhibition test , according to OECD 209, Equamide M100 was tested for its effect on activated domestic sludge. Nominal concentrations of 10, 100 and 1000 mg/L were used, together with a control. The reference substance, 3,5 -dichlorophenol was tested at concentrations of 3.2, 10, 32 and 100 mg/L and an abiotic control (containing no sludge) was included as well. Results showed that Equamide M100 did not inhibit respiration up to the highest tested concentration.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
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