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EC number: 207-803-7 | CAS number: 495-54-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02-05-2017 - 05-05-2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- The stock solution (10 mg/L) was prepared by dissolving yellow powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: -
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available
ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- ±1 hour
- Test temperature:
- 23 ± 2°C
- pH:
- Without adjustment
The sample at concentration 0.80 mg/L did not change during the tests
Control: pH= 8.0 changed to pH 7.4 during the test - Nominal and measured concentrations:
- 0, 0.5, 0.10, 0.20, 0.40 and 0.80 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.17 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 0.16 - 0.19 mg/L
- Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.75 mg/L - Validity criteria fulfilled:
- not specified
- Conclusions:
- The median effective concentration (EC50) for the test substance, 4-(phenylazo)benzene-1,3-diamine, in Desmodesmus subspicatus was determined to be 0.17 mg/L on the basis of effects on growth rate in a 72 hour study.
- Executive summary:
Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 4-(phenylazo)benzene-1,3-diamine according to OECD Guideline 201.
The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 0.5, 0.10, 0.20, 0.40 and 0.80 mg/L. Effects on the growth rate of the organism were studied.
The median effective concentration (EC50) for the test substance, 4-(phenylazo)benzene-1,3-diamine, in Desmodesmus subspicatus was determined to be 0.17 mg/L.
This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic acute category 1 as per the CLP criteria.
Reference
Description of key information
The median effective concentration (EC50) for the test substance, 4-(phenylazo)benzene-1,3-diamine, in Desmodesmus subspicatus was determined to be 0.17 mg/L on the basis of effects on growth rate in a 72 hour study.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.17 mg/L
Additional information
Different studies, experimental and predicted, of the substance4-(phenylazo)benzene-1,3-diamine were reviewed for Short term toxicity to aquatic algae and cyanobacteria endpoint and are summarised as follows:
Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 4-(phenylazo)benzene-1,3-diamineaccording to OECD Guideline 201. The test substance was dissolved in OECD growth medium and tested at the concentrations0, 0.5, 0.10, 0.20, 0.40 and 0.80 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,4-(phenylazo)benzene-1,3-diamine, in Desmodesmus subspicatus was determined to be 0.17 mg/L.
Supporting data from other lab indicates, the effect of test item 4-(phenylazo)benzene-1,3-diamine, CAS No. 495-54-5 was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 0.9 mg/L,2.7 mg/L,8.1 mg/L,24.3 mg/L,72.9 mg/L,218.7 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 10.07mg/L.
Further supporting data is based on the QSAR prediction done using the EPI Suite ECOSAR version 1.11, the 72 hours EC50 was estimated to be 0.653 mg/L on algae for substance 4-(Phenylazo)benzene-1,3-diamine with growth inhibition effects.
Thus considering the key and supporting values, the EC50 value is observed to be in the range 0.17 - 10.07 mg/L. But considering the harmonised classification of the substance and for the purpose of classification and chemical safety assessment the substance 4-(phenylazo)benzene-1,3 -diamine is considered to classified as Aquatic acute 1 and aquatic chronic 1 category.
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