Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-025-4 | CAS number: 90-93-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09/02/2018 to 29/03/2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Common name: Omnirad EMK
- CAS number: 90-93-7
- Source and lot/batch No.of test material: 177116
- Expiration date of the lot/batch: 16/11/2019
- Purity test date: 99.50%
- Purity: 16/11/2017
- Appearance: white to pale yellow green powder
- Test handling: use amber glassware or wrap container in foil
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature protected from light
PREPARATION OF STOCK SOLUTIONS
- Method of addition: since the test substance was not sufficiently soluble to alllow preparation of an aqueous solution at a concentration of 1g/L, weighed amounts were added to the 2 litre test vessels containing mineral medium. 31.17, 31.15, 30.88 mg were added to test vessels A, B and the toxicity control bottle. - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge freshly obtained from a municipal sewage treatment plant, Waterschap Aa en Maas, s-Hertogenbosch. Treats predominantly domestic sewage.
- Preparation of inoculum for exposure: Sludge was used immediately. Before use, the sludge was washed with mineral medium. Magnetically stirred.
- Concentration of sludge: After treatment the concentration of suspended solids was determined at 3.2 g/L
- Initial cell/biomass concentration: not stated
- Water filtered: not stated - Duration of test (contact time):
- 28 d
- Initial conc.:
- 16 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium made up using 4 solutions as follows:
Solution A: 8.5g KH2PO4, 21.75g K2HPO4, 67.20g Na2HPO4.12H2O, 0.5g NH4Cl, dissolved in Milli-RO water and made upto 1L, pH 7.4 ± 0.2.
Solution B: 22.50g MgSO4.7H2O dissolved in Milli-RO water and made up to 1L.
Solution C: 36.40g CaCl2.2H2O dissolved in Milli-RO water and made up to 1L
Solution D: 0.25g FeCl3.6H2O dissolved in Milli-RO water and made upto 1L.
Final mineral medium prepared using 10ml solution A, 1ml solution B, C and D. Made upto 1L in Milli-RO water.
- Test temperature: 22 to 23°C
- pH: ranged from 7.5 to 7.8 in all of the test, reference and toxicity control vessels.
- pH adjusted: adjustment carried out at the start of the test in one of the blank control vessels only.
- CEC (meq/100 g): not stated
- Suspended solids concentration: addition of 3ml per litre of mineral medium, resulting in a SS of 9.6 mg/L
- Continuous darkness: glass brown bottles used. Test media exluded from the light.
- Other: the test media was aerated and stirred continuously. Tap water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon used.
TEST SYSTEM
- Culturing apparatus: 2 litre brown glass bottles
- Number of culture flasks/concentration: 2 flasks, 1 concentration
- Method used to create aerobic conditions: continual aeration and stirring
- Test performed in closed vessels due to significant volatility of test substance: not stated
- Test performed in open system: not stated
- Details of trap for CO2 and volatile organics if used: three CO2 absorbers (bottles filled with 100ml 0.0125 MBa(OH)2 were connected in series to the exit air line of each test bottle.
- Other: At the start of the test (day 0), test and reference item were added to the bottles containing the microbial organisms and mineral components. The volumes of suspensions were made up to 2 litres with Milli- RO water. Three CO2-absorbers (bottles filled with 100 mL 0.0125 M Ba(OH)2) were connected in series to the exit air line of each test bottle.
SAMPLING
- Sampling frequency: 2, 5, 8, 12, 15, 19, 23, 28 (measured on day 29)
- Sampling method: titrations were made on the sampling day. Each time the CO2 absorber nearest to the test bottle was removed for titration, each of the remaining two absorbers were moved one position in the direction of the test bottle. A new CO2 absorber was placed at the far end of the series. On the penultimate day, the pH of the respective test suspensions was measured and 1ml of concentrated HCl added to the bottles. The bottles were then aerated overnight to drive off the CO2 present in the test suspension.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 bottles containing inoculum only
- Abiotic sterile control: Not included
- Toxicity control: 1 bottle containing test item, reference item and inoculum.
- Reference control: 1 bottle containing reference item and inoculum. - Reference substance:
- other: sodium acetate
- Remarks:
- see below for further details
- Preliminary study:
- Not carried out
- Test performance:
- The performance of the test was valid and met all the criteria as detailed below.
1. The reference item was biodegraded by at least 60% (84%) within 14 days.
2. The difference of duplicate values for %-degradation of the test item was always less than 20 (≤ 8%).
3. The total CO2 release in the blank at the end of the test did not exceed 40 mg/L (63.6 mg CO2 per 2 litres of medium, corresponding to 31.8 mg CO2/L).
4. The Inorganic Carbon content (IC) of the test item (suspension) in the mineral medium at the beginning of the test was less than 5% of the Total Carbon content (TC). Since the test medium was prepared in tap-water purified by reverse osmosis (Milli- RO water (Millipore Corp., Bedford, Mass., USA, carbon levels < 500 ppb)), IC was less than 5% of TC (mainly coming from the test item, 12 mg TOC/L). - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: Bottle A
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 8
- Sampling time:
- 28 d
- Remarks on result:
- other: Bottle B
- Details on results:
- The average of biodegradation at day 28 was 4%. See Tables 1,3,4,5 and 6 below for further results.
- Results with reference substance:
- The biodegradation with the reference substance was measured at 12, 34, 50, 61, and 70% at days 2, 5, 8, 12 and 14. See Table 2 for further details.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The relative biodegradation values calculated from the measurements performed during the test period revealed no biologically relevant biodegradation of 4,4’- Bis(diethylamino)benzophenone (0% and 8%, based on ThCO2). The test substance was classifed as not readily biodegradable.
- Executive summary:
The biodegradation potential of 4,4’- Bis(diethylamino)benzophenone was assessed using an OECD 301B studydesign. The test was carried out accoring to the guideline and according to GLP. The study is considered a Klimisch 1 study. The biodegradation of the test substance was measured over a 28 day period (final measurement made on day 29, after the reaction was stopped on day 28), using a titration method to assess carbon dioxide production. A reference control, background control and toxicity control were all included. The reference control indicated adequate biodegradation in the reference control vessels, demonstrating the adequate functioning of the inoculum. No toxicity to the bacteria was observed.
After 28 days the percentage biodegradation calculated in the test vessels for 4,4’- Bis(diethylamino)benzophenone was 0 and 8% for the two test vessels respectively. Overall, the test substance was concluded not to be readily biodegradable.
Reference
Table 1: CO2 production in the blank.
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
|
|
Ba(OH)21) |
Blank (mean) |
|
||||
2 |
49.40 |
46.16 |
3.24 |
3.6 |
3.6 |
|
5 |
49.44 |
43.62 |
5.82 |
6.4 |
10.0 |
|
8 |
49.23 |
41.83 |
7.40 |
8.1 |
18.1 |
|
12 |
49.87 |
42.26 |
7.62 |
8.4 |
26.5 |
|
15 |
49.76 |
43.56 |
6.19 |
6.8 |
33.3 |
|
19 |
50.13 |
43.58 |
6.55 |
7.2 |
40.5 |
|
23 |
49.85 |
44.00 |
5.85 |
6.4 |
46.9 |
|
292) |
52.06 |
44.30 |
7.76 |
8.5 |
55.5 |
|
292) |
52.27 |
47.75 |
4.52 |
5.0 |
60.4 |
|
292) |
52.45 |
49.55 |
2.91 |
3.2 |
63.6 |
|
1): "Strength" of untreated 0.0125 M Ba(OH)2solution 2): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl. |
Table 2. CO2 production and percentage biodegradation of the reference item
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
Blank (mean) |
Procedure control |
||||||
2 |
46.16 |
27.14 |
19.02 |
20.9 |
20.9 |
12 |
|
5 |
43.62 |
8.19 |
35.43 |
39.0 |
59.9 |
34 |
|
8 |
41.83 |
17.19 |
24.64 |
27.1 |
87.0 |
50 |
|
12 |
42.26 |
24.18 |
18.08 |
19.9 |
106.9 |
61 |
|
152) |
43.56 |
29.04 |
14.52 |
16.0 |
122.8 |
70 |
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of sodium acetate: 86.3 mg CO2/2L. 2): CO2measured on day 15 is actually part of CO2production of day 14, since microbial activity was ended on day 14 by addition of HCl. |
|
Table 3. CO2 production and percentage biodegradation of the test item (bottle A)
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
Blank (mean) |
Bottle A |
||||||
2 |
46.16 |
46.49 |
0.00 |
0.0 |
0.0 |
0 |
|
5 |
43.62 |
44.28 |
0.00 |
0.0 |
0.0 |
0 |
|
8 |
41.83 |
44.23 |
0.00 |
0.0 |
0.0 |
0 |
|
12 |
42.26 |
43.39 |
0.00 |
0.0 |
0.0 |
0 |
|
15 |
43.56 |
44.70 |
0.00 |
0.0 |
0.0 |
0 |
|
19 |
43.58 |
44.46 |
0.00 |
0.0 |
0.0 |
0 |
|
23 |
44.00 |
44.70 |
0.00 |
0.0 |
0.0 |
0 |
|
292) |
44.30 |
45.43 |
0.00 |
0.0 |
0.0 |
0 |
|
292) |
47.75 |
48.10 |
0.00 |
0.0 |
0.0 |
0 |
|
292) |
49.55 |
50.26 |
0.00 |
0.0 |
0.0 |
0 |
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test item: 88.8 mg CO2/2L. 2): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl. |
|
Table 4. CO2 production and percentage biodegradation of the test item (Bottle B)
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
Blank (mean) |
Bottle B |
||||||
2 |
46.16 |
46.34 |
0.00 |
0.0 |
0.0 |
0 |
|
5 |
43.62 |
43.12 |
0.50 |
0.6 |
0.6 |
1 |
|
8 |
41.83 |
42.30 |
0.00 |
0.0 |
0.6 |
1 |
|
12 |
42.26 |
43.26 |
0.00 |
0.0 |
0.6 |
1 |
|
15 |
43.56 |
43.13 |
0.43 |
0.5 |
1.0 |
1 |
|
19 |
43.58 |
41.10 |
2.48 |
2.7 |
3.7 |
4 |
|
23 |
44.00 |
42.88 |
1.12 |
1.2 |
5.0 |
6 |
|
292) |
44.30 |
43.64 |
0.66 |
0.7 |
5.7 |
6 |
|
292) |
47.75 |
46.39 |
1.36 |
1.5 |
7.2 |
8 |
|
292) |
49.55 |
49.23 |
0.32 |
0.3 |
7.5 |
8 |
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test item: 88.8 mg CO2/2L. 2): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl. |
|
Table 5. CO2 production and percentage biodegradation of the toxicity control.
Day |
HCl(0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
|
Blank (mean) |
Toxicity control |
|||||
2 |
46.16 |
30.34 |
15.82 |
17.4 |
17.4 |
10 |
5 |
43.62 |
22.21 |
21.41 |
23.6 |
40.9 |
23 |
8 |
41.83 |
30.73 |
11.10 |
12.2 |
53.2 |
30 |
12 |
42.26 |
35.49 |
6.76 |
7.4 |
60.6 |
35 |
152) |
43.56 |
38.23 |
5.33 |
5.9 |
66.5 |
38 |
1): Calculated as the ratio between CO2produced (cumulative) and the sum of the ThCO2of the test item and reference item: 174.3 mg CO2/2L (ThCO2test item: 88.0 mg CO2/2L + ThCO2sodium acetate: 86.3 mg CO2/2L). 2): CO2measured on day 15 is actually part of CO2production of day 14, since microbial activity was ended on day 14 by addition of HCl. |
Table 6. Comparison of biodegradation of the test item in bottles A and B.
Day |
Biodegradation (%) |
|||
Bottle A |
Bottle B |
Mean A and B |
∆ A-B1) |
|
2 |
0 |
0 |
0 |
0 |
5 |
0 |
1 |
1 |
1 |
8 |
0 |
1 |
1 |
1 |
12 |
0 |
1 |
1 |
1 |
15 |
0 |
1 |
1 |
1 |
19 |
0 |
4 |
2 |
4 |
23 |
0 |
6 |
3 |
6 |
292) |
0 |
6 |
3 |
6 |
292) |
0 |
8 |
4 |
8 |
292) |
0 |
8 |
4 |
8 |
1): Absolute difference in biodegradation between bottles A and B 2): Biodegradation is ended on day 28 by addition of HCl. Therefore, differences observed on day 29 are actually differences of day 28. |
Description of key information
The biodegradation potential of 4,4’- Bis(diethylamino)benzophenone was assessed using an OECD 301B study test design. The test was carried out according to the guideline and GLP. The study is considered a Klimisch 1 study. The biodegradation of the test substance was measured over a 28 day period (final measurement made on day 29, after the reaction was stopped on day 28), using a titration method to assess carbon dioxide production. A reference control, background control and toxicity control were all included. The reference control indicated adequate biodegradation in the reference control vessels, demonstrating the adequate functioning of the inoculum. No toxicity to the bacteria was observed.
After 28 days the percentage biodegradation calculated in the test vessels for 4,4’- Bis(diethylamino)benzophenone was 0 and 8% for the two test vessels, respectively. Overall, the test substance was concluded not to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- other: domestic activated sludge
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.