Bronopol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- Stock solutions of the test material ware prepared in distilled water. One litre of each test solution was prepared by direct addition of aliquots of stock solution to dilution water.
- The dilution water was aerated for > 2 hours before use. The pH of the dilution water was 8.25 ± 0.25.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Test organism: Daphnia magna STRAUS, obtained from continuous laboratory cultures
- Holding: the stock cultures of Daphnia were maintained in a reconstituted water medium, identical to the test dilution water, at a temperature of 20.0 +/- 1°C and a photoperiod of 16 h light:8 h dark
- Feeding: the cultures were fed a defined diet of algae (Chlorella vulgaris) and yeast
- Daphnia 12 +/- 12 hours old were used for testing

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

19.5 - 20.0°C

pH:

8.20 to 8.40

Dissolved oxygen:

8.4 to 9.4 mg/L

Nominal and measured concentrations:

- Concentrations tested (nominal): 0.032, 0.056, 0.1, 0.18, 0.32, 0.56, 1.0, 3.2 and 5.6 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessels: Borosilicate glass beakers
- Test volume: 200 ml of test solution
- Replicates per concentration: 4
- Organisms per replicate: 5
- Organisms per concentration: 20

FURTHER TEST CONDITIONS
- Photoperiod: 16 h light:8 h dark
- Light intensity: ca. 1500 lux
- Feeding: none

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

EC50 (24 h) = 2.9 mg/L

Validity criteria fulfilled:

yes

Description of key information

Acutely toxic for aquatic organisms.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

1.4 mg/L

Marine water invertebrates

Marine water invertebrates

Dose descriptor:

EC50

Effect concentration:

3.5 mg/L

Additional information

The acute toxicity of Bronopol was examined in a static test system according to methods comparable to OECD guideline 202 with Daphnia magna as test organism [The Boots Company 1981]. The 48 h EC50 value was determined to be 1.4 mg/L (nominal).

A supporting study is available which was conducted according to ISO guideline 14669 with the saltwater species Acartia tonsa. At test termination after 48 h an EC50 of 3.5 mg/L (nominal) was determined [Knoll MicroCheck 1998].