Benzeneacetonitrile, 4-chloro-.alpha.-[(3-chloro-2-fluorophenyl)methylene]-2-fluoro-, (.alpha.Z)-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 September 2015-14 October 2015

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(2Z)-3-(3-chloro-2-fluorophenyl)-2-(4-chloro-2-fluorophenyl)prop-2-enenitrile

EC Number:

806-533-2

Cas Number:

1219086-87-9

Molecular formula:

C15 H7 Cl2 F2 N

IUPAC Name:

(2Z)-3-(3-chloro-2-fluorophenyl)-2-(4-chloro-2-fluorophenyl)prop-2-enenitrile

Details on test material:

Lot no. FIS201405080058
Molecular formula C15H7Cl2F2N
Molecular mass 310.13 g/mol
Physical form solid
Purity 101.2 % (m/m)
Water solubility <0.02 mg/L at 20°C and pH 4.4 – 6.0, as determined in an OECD 105 test (ACH study no, A15-01329)

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.02 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The effective concentrations (ErC10, ErC20, ErC50 and EyC10, EyC20, EyC50) as well as the no observed effect concentrations (NOErC and NOEyC), PAC-Ethenenitrile (CAS no. 1219086-87-9) to the green algae Desmodesmus subspicatus, were investigated over a period of 72 h, based on the average specific growth rate and the yield (algal biomass production). The test item PAC-Ethenenitrile is solid, pure and not poorly soluble. Consequently the test medium was prepared by adding the respective amount of test item to empty glass vessels, followed by addition of the Algal medium, overnight stirring and filtration. The single investigated loading rate was 100 mg/l. Six replicate test vessels were used for the test concentration, and six for the blank controls. The concentrations of PAC-Ethenenitrile in the test medium were measured by HPLC analysis at the beginning and after 24, 48 h and 72 h of exposure. These analyses confirmed that the test item is poorly soluble (all measurements below the limit of quantification (LOQ) of 0.02 mg/l). Additional- ly, DOC analysis of the test concentration was performed in the test solution at the beginning of the test. These analyses confirmed the results of the HPLC analysis (all measurements were below the limit of quantification (LOQ) of 0.5 mg/l). No significant effects were observed at 100 mg/l loading rate as compared to the blank controls, neither based on the growth rate, nor based on the yield. Therefore, the ErC10, ErC20, ErC50 and EyC10, EyC20, EyC50 values of PAC-Ethenenitrile on the green alga Desmodesmus subspicatus were all estimated to be >100 mg/l loading rate, or >0.02 mg/l measured concentration. The NOErC and NOEyC values are both 100 mg/l loading rate, or 0.02 mg/l measured concentration. All validity criteria were fulfilled.