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EC number: 221-897-7 | CAS number: 3271-76-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- Start of experimental phase: 23 June 2017;End of experimental phase 24 July 2017; Study completion:11 December 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- Adopted July 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- EC Number:
- 221-897-7
- EC Name:
- Anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- Cas Number:
- 3271-76-9
- Molecular formula:
- C31H15NO3
- IUPAC Name:
- anthra[2,1,9-mna]naphth[2,3-h]acridine-5,10,15(16H)-trione
- Test material form:
- solid: particulate/powder
Constituent 1
Method
- Target gene:
- the test item for the ability to induce gene mutations in Salmonella typhimurium and Escherichia coli, as measured by reversion of auxotrophic strains to prototrophy
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Details on mammalian cell type (if applicable):
- Permanent stocks of these strains are kept at -80°C in RTC. Overnight subcultures of these
stocks were prepared for each day’s work. Bacteria were taken from vials of frozen cultures,
which had been checked for the presence of the appropriate genetic markers, as follows:
Histidine requirement No Growth onMinimal plates+Biotin.Growth onMinimal plates+Biotin+Histidine.
Tryptophan requirement No Growth onMinimal agar plates.Growth onMinimal plates+Tryptophan.
-uvrA, uvrB : Sensitivity to UV irradiation.
-rfa : Sensitivity to Crystal Violet.
- pKM101: Resistance to Ampicillin.
Bacterial cultures in liquid and on agar were clearly identified with their identity
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver S9 fraction from rats pre-treated with phenobarbital and 5,6-benzoflavone.
- Test concentrations with justification for top dose:
- Preliminary Toxicity test: 2500, 791, 250, 79.1 and 25.0 μg/plate.
Main Assay I: 2000, 1000, 500, 250 and 125 μg/plate.
Main Assay II ( confirmatory experiment): 2000, 1000, 500,250 and 125 μg/plate with TA1537 and TA98 tester strains in the presence of S9 metabolism. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: compatible with the survival of the bacteria and the S9 metabolic activity.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- methylmethanesulfonate
- other: 2-aminoanthracene
- Remarks:
- Marked increases in revertant numbers were obtained in these tests following treatment with the positive control items, indicating that the assay system was functioning correctly.
- Details on test system and experimental conditions:
- The preliminary toxicity test and the Main Assay I and II were perfomed using a plate-incorporation method.
- Evaluation criteria:
- For the test item to be considered mutagenic, two-fold (or more) increases in mean revertant numbers must be observed at two consecutive dose levels or at the highest practicable dose level only. In addition, there must be evidence of a dose-response relationship showing increasing numbers of mutant colonies with increasing dose levels.
- Statistics:
- Doubling rate ( Chu et al. 1981); Regression line
Results and discussion
Test results
- Key result
- Species / strain:
- other: S.typhimurium TA1535, TA1537, TA98 and TA100; E.coli WP2 uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- the test item Vat Green 3 induces reverse mutation by frameshifts in Salmonella typhimurium in the presence of S9 metabolism, under the reported experimental conditions
Applicant's summary and conclusion
- Conclusions:
- It is concluded that the test item Vat Green 3 induces reverse mutation by frameshifts in Salmonella typhimurium in the presence of S9 metabolism, under the reported experimental conditions.
- Executive summary:
The test item Vat Green 3 was examined for the ability to induce gene mutations in tester strains of Salmonella typhimurium and Escherichia coli, as measured by reversion of auxotrophic strains to prototrophy. The five tester strains TA1535, TA1537, TA98, TA100 and WP2 uvrA were used. Experiments were performed both in the absence and presence of metabolic activation, using liver S9 fraction from rats pre-treated with phenobarbital and 5,6-benzoflavone.
The test item was used as a solution in dimethylsulfoxide (DMSO).
Toxicity test
The test item Vat Green 3 was assayed in the toxicity test at a maximum concentration of
2500 μg/plate and at four lower concentrations spaced at approximately half-log intervals:
791, 250, 79.1 and 25.0 μg/plate.
Two-fold increases in revertant numberswere observed with TA1537 and TA98 tester strains in
the presence of S9 metabolic activation at the highest or three highest dose levels, respectively.
Dose-related precipitation of the test item was observed with all tester strains at the two highest dose levels, both in the absence and presence of S9 metabolism. At 2500 μg/plate, the abundant precipitate interfered with the scoring of the background lawn and the resulting evaluation of possible toxic effects at this dose level. No toxicity was observed at lower dose levels with any tester strain, in the absence or presence of S9 metabolic activation. Based on these results, 2000 μg/plate was selected as the top dose to be used in Main Assay I.
Main Assays
In Main Assay I, using the plate incorporation method, the test item was assayed at the following dose levels: 2000, 1000, 500, 250 and 125 μg/plate. Dose-related precipitation of the test item was seen at the two highest dose levels both in the absence and presence of S9 metabolic activation. At the end of the incubation period, no toxicity of the test item was observed, with any tester
strain, at any dose level, in the absence or presence of S9 metabolism. Dose-related increases in revertant numbers were observed with TA1537 (2.4-fold) and TA98 (5.3-fold) tester strains in the presence of S9 metabolism. A confirmatory experiment (Main Assay II) was performed, in which TA1537 and TA98 tester strains were treated in the presence of S9 metabolism at the dose levels of 2000, 1000, 500, 250 and 125 μg/plate. Dose-related precipitation of the test item was seen at the two highest dose levels. Dose-related increases in revertant colonies were reproduced for both tester strains. The number of revertant colonies after TA1537 exposure did not reach twice the concurrent negative control values, although it fell out the historical control range for this
tester strain at higher concentrations. A clear positive effect was confirmed with TA98 tester strain, where numbers of revertant colonies over two fold (up to 4.95) the concurrent vehicle control were observed at all dose levels.
Since an obvious positive response was observed, no further experiment was undertaken.
Conclusion
It is concluded that the test item Vat Green 3 induces reverse mutation by frameshifts in Salmonella typhimurium in the presence of S9 metabolism, under the reported experimental conditions.
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