Registration Dossier

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions (e.g. non-GLP)

Data source

Reference
Reference Type:
review article or handbook
Title:
Biodegradation and bioconcentration of existing chemical substances under the chemical substances control law: 1-phenylethylamine (CAS 98-84-0)
Author:
NITE
Year:
2011
Bibliographic source:
National Institute of Technology and Evaluation, Japan; http://www.safe.nite.go.jp/data/sougou/pkc_e_search_frm.html; Data first published: 25 Dec 1981
Report Date:
2011

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: "Bioaccumulation test of chemical substance in fish and shellfish" in Prescribing the Items of the Test Relating to the New Chemical Substance (1974, Order of the Prime Minister of Health and Welfare, MITI No.1). This guideline corresponds to OECD 305 C.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1-phenylethylamine
- Analytical purity: No data
Radiolabelling:
no

Sampling and analysis

Details on sampling:
- Sampling intervals/frequency for test organisms: every two weeks (n = 2)
- Sampling intervals/frequency for test medium samples: 2x/week (n = 1)
- The controls were analysed before initiation and termination of exposure (n = 2).

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: carp
- Source: Sugishima fish farm (Japan)
- Age at study initiation (mean and range, SD):
- Length at study initiation (lenght definition, mean, range and SD): ca. 10 cm
- Weight at study initiation (mean and range, SD): ca. 30 g
- Lipid content: 2-6 %
- Health status: removal of abnormal fish during acclimation period
- Description of housing/holding area: flow-through system, 25±2 °C, 28 d
- Feeding during test
- Food type: pelleted feed for carp manufacturing
- Amount: 2 % of total body weight of the test fish
- Frequency: 2 times a day (no feeding on day of fish sampling)


ACCLIMATION
- Acclimation period: 28 d
- Acclimation conditions (same as test or not): yes
- Type and amount of food: same as test
- Feeding frequency: same as test
- Health during acclimation (any mortality observed): no data (abnormal fish were removed during acclimation period)

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
6 wk

Test conditions

Test temperature:
25±2 °C
Dissolved oxygen:
6-8 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100-L glass tank
- Renewal rate of test solution (frequency/flow rate):200-800 mL/min
- No. of organisms per vessel: 15-20


TEST MEDIUM / WATER PARAMETERS
- Holding medium different from test medium: no


RANGE-FINDING / PRELIMINARY STUDY
- Results used to determine the conditions for the definitive study: LC50(48 h, Oryzias latipes): 48 mg/L
Nominal and measured concentrations:
nominal: 0.05 and 0.5 mg/L

Results and discussion

Lipid content
Lipid content:
4.4 %
Time point:
start of exposure
Bioaccumulation factoropen allclose all
Type:
BCF
Value:
1 - 2.9
Remarks on result:
other: Conc.in environment / dose:0.5 mg/L
Type:
BCF
Value:
< 6.6
Remarks on result:
other: BCF: < 2.6 to 6.6
Remarks:
Conc.in environment / dose:0.05 mg/L

Applicant's summary and conclusion