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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Acrylamide
EC Number:
201-173-7
EC Name:
Acrylamide
Cas Number:
79-06-1
Molecular formula:
C3H5NO
IUPAC Name:
prop-2-enamide
Details on test material:
- Name of test material (as cited in study report): CT 444 90 D
- Substance type: organic
- Physical state: White, crystalline solid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: 100%
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch:
- Stability under test conditions: stable
- Storage condition of test material: Stored in original, sealed container at 4° C

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
Diluent: US EPA moderately hard reconstituted
Solvent: None required
Controls: Diluent only

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Strain: Onchorhyncus mykiss
- Source: Aquatic Research organisms, Hampton, NH
- Age at study initiation: 64 days (Hatch: 11/09/90)
- Length at study initiation: ≤50 mm total length, uniform size
- Feeding during test: None
ACCLIMATION:Test fish were acclimated to the receiving water for a minimum of 24 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
None

Test conditions

Hardness:
80-100 mg/L as CaC03.
Test temperature:
15°C±2°C
pH:
7.4 to 7.8.
Dissolved oxygen:
7.4-8.6
Nominal and measured concentrations:
Nominal : 0, 62.5, 125, 250, 500, 1000 ppm
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 4L polypropylene vessels; exposure volume 3000 ml
- Aeration: free air supplied at ≤100 bubbles per minute to maintain ≥ 60% saturation.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: EPA moderately hard reconstituted (from EPA 600/4-85/013):
Reconstituted freshwater
NaHCO3 = 96 mg/L
CaSO4.2H20 = 60 mg/L
MgSO4 = 60 mg/L
KCI = 4 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16hr/8hr light/dark cycle
- Light intensity: fluorescent, 50 to 100 foot-candles (lab ambient)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, behaviour (lethargy, erratic swimming), and physiology (discoloration), initially, and daily thereafter.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
500 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: not obtainable graphical interpolation
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
360 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: not obtainable graphical interpolation
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
240 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: not obtainable graphical interpolation
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
180 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: not obtainable graphical interpolation

Any other information on results incl. tables

Sublethal observations / clinical signs:

Acute Toxicity versus Onchorhyncus mykiss Definitive Test - CT 444 90 D

 Conc.( ppm)  Number Organism      Cumulative Mortality       
     24 h  48 h  72 h  96 h  % Mortality
 0 control  10  0  0  0  0  0
 62.5  10  0  0  0  1  10
 125  10  0  0  0  0  0
 250  10  0  0  6  10  100
 500  10  5  10  10  10  100
 1000  10  10  10 10  10  100

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
all of control organisms survived 96 hours
Conclusions:
Acute toxicity of acrylamide (CT 444 90 D) to the Rainbow Trout, Onchorhyncus mykiss, was determined to be 180 ppm.