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EC number: 214-317-9 | CAS number: 1120-71-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- publication
- Title:
- CHROMOSOME TESTS WITH 134 COMPOUNDS ON CHINESE HAMSTER CELLS IN VITRO - A SCREENING FOR CHEMICAL CARCINOGENS
- Author:
- Ishidate, M. et al.
- Year:
- 1 977
- Bibliographic source:
- Mutation Research, 48 337-354
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- not applicable
- GLP compliance:
- no
- Type of assay:
- other: in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 1,3-propanesultone
- EC Number:
- 214-317-9
- EC Name:
- 1,3-propanesultone
- Cas Number:
- 1120-71-4
- Molecular formula:
- C3H6O3S
- IUPAC Name:
- 1,2λ⁶-oxathiolane-2,2-dione
Constituent 1
Method
- Target gene:
- not applicable
Species / strain
- Species / strain / cell type:
- mammalian cell line, other: Chinese hamster lung fibroblast cell line (CHL)
- Details on mammalian cell type (if applicable):
- - originally established from the lung of a young adult by Dr. T. Utakoji, Cancer Institute, Tokyo
- karyotype consists of 25 chromosomes
- maintained by 5-day passages
- grown in a monolayer in petri dishes with Eagle's MEM (GIBCO F-11) supplemented with 10 % calf serum
- doubling time was estimated as 18.2 h at their exponential growth at 37 °C in a 5 % CO2 atmosphere
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- 0.0313 , 0.0625 or 0.125 mg/mL
- Vehicle / solvent:
- - physiological saline
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- solvent: physiological saline
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: 24 and 48 h
- Fixation time (start of exposure up harvest of cells): 24 and 48 h
SPINDLE INHIBITOR (cytogenetic assays): colcemid (0.2 µg/mL; added to the medium 2 h before harvest)
STAIN (for cytogenetic assays): 1% Giemsa's buffered solution (pH 6.8)
NUMBER OF REPLICATIONS: no data
NUMBER OF CELLS EVALUATED: the number of cells with chromosomal aberrations was recorded on 100 well-spread metaphases
DETERMINATION OF CYTOTOXICITY
- only examinded in a pre-test (growth inhibition test)
- the dose inducing a 50% growth inhibition was taken as the highest dose in the main test
Growth inhibition tests carried out before the chromosome tests were started:
The 50% growth inhibition dose was estimated as follows:
Several different doses of each agent were separately added to the 3-day-old cultures (about 6 x 10E3 cells/3-cm dishes). The doses were prepared by a factor of 2 from the maximal dose, estimated from the data on LD50, which appeared in references for the test item. The cells in a monolayer were washed, fixed with 10% formalin solution, and then stained with 0.1% crystal violet solution for 3 min. After washing and drying each dish was placed under a photodensitometer to measure the color absorption values from which relative cell densities on the dishes were easily calculated. The color absorption values obtained reflected well the actual number of cells that survived at the bottom of each petri dish.
OTHER EXAMINATIONS:
- Determination of polyploidy: yes
OTHER:
- types of aberration were classified into 5 groups: chromatid gaps, chromatid breaks, chrorrmatid or chromosomal translocation, ring formation and fragmentation or pulverization - Evaluation criteria:
- CHL cells commonly have less than 3.0% cells with chromosomal aberrations. Therefore, the final judgement given to all experimental groups was as follows: Negative if less than 4.9% of the aberration was detected – even when doses of the agent were elevated to sub-lethal amounts, where almost no mitosis was observed; suspicious if between 5.0 and 9.9%, and positive if between 10.0 and 19.9% (+), 20.0 and 49.9% (++) and more than 50.0% (+++). When no reasonable dose response was obtained, experiments with different doses were carried out to confirm its reproducibility.
- Statistics:
- no data
Results and discussion
Test results
- Key result
- Species / strain:
- mammalian cell line, other: Chinese hamster fibroblast cell line (CHL)
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- 94% cells with abberations
- Cytotoxicity / choice of top concentrations:
- other: cytotoxicity can be assumed as the dose where 50% growth inhibition occured in the pre-test was used as the highest dose
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- not examined
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- no data
COMPARISON WITH HISTORICAL CONTROL DATA:
- CHL cells commonly have less than 3.0% cells with chromosomal aberrations. This value was taken for judgement.
Any other information on results incl. tables
A D20 value (the dose (mg/mL) at which chromosomal aberrations were detected in 20% of metaphases) of 0.0451 mg/mL was obtained.
Results chromosome test (only 24 values are presented):
Dose [mg/mL] |
No of Metaphases |
Polyploid cells [%] |
Cells with structural chromosome aberrations [%], 24 h |
|||||||
|
|
24 h |
48 h |
G |
B |
T |
R |
F |
Total |
|
|
|
|
|
|
|
|
|
|
|
|
None |
100 |
1 |
- |
1 |
0 |
0 |
0 |
0 |
1 |
|
Solv |
|
- |
- |
- |
- |
- |
- |
- |
- |
|
0.0313 |
100 |
0 |
1 |
1 |
3 |
2 |
0 |
0 |
6 |
|
0.0625 |
100 |
1 |
0 |
2 |
7 |
16 |
0 |
0 |
22 |
|
0.125 |
100 |
1 |
T |
0 |
46 |
91 |
0 |
0 |
94 |
G: chromatid gaps
B: chromatid or chromosomal breaks
T: translocation
R: ringformation
F: fragmentation
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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