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Short-term toxicity to aquatic invertebrates

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Description of key information

The short-term toxicity of the substance to aquatic invertebrates was determined in a study in accordance with OECD TG 202. In this study the 48-h EC50 was determined at 82 mg/L (measured concentration).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
82 mg/L

Additional information

The short-term toxicity of the substance to aquatic invertebrates was determined in a study in accordance with OECD TG 202 and in compliance with GLP criteria (Brixham Envriomental laboratory, 2010). In this study, Daphnia magna (4 replicates of 5 animals per concentration) were exposed to nominal concentrations of 0, 4.38, 8.75, 17.5, 35 and 70% of saturation for 48 hours under static conditions. Nominal concentrations were prepared by bubbling test substance through 3500 mL of media for approximately 60 minutes to obtain a saturated solution of the gas (100 %). This was then diluted to 70 % saturation with media that had been diffused with oxygen for 30 minutes. All other concentrations were prepared from the 70 % stock. Test concentrations were analytical verified. Mean measured concentrations were 27, 38, 91, 175 and 405 mg/L. After 48 hours exposure, an effect was first seen at 38 mg/L with 5% immobilisation. At 91 and 175 mg/L, immobilisation percentages were 65 and 95%, respectively and at the highest concentration tested all Daphnia were immobilised. Based on these findings, the 48 -h EC50 was determined at 82 mg/L (measured concentration).