Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 953-797-0 | CAS number: 210300-09-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 11268-2 (Effects of Pollutants on Earthworms. 2. Determination of Effects on Reproduction)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-hydroxy-4-methoxypyridine-2-carboxylic acid
- Cas Number:
- 210300-09-7
- Molecular formula:
- C7H7NO4
- IUPAC Name:
- 3-hydroxy-4-methoxypyridine-2-carboxylic acid
Constituent 1
- Specific details on test material used for the study:
- Substance name: X696476
Lot #: XS9-109113-80 (TSN300901)
Purity: 100%
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
- Remarks:
- deionized water
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Total exposure duration:
- 8 wk
Test conditions
- Test temperature:
- 18 to 22°C
- pH:
- pH at initiation: 5.7 to 5.9
pH at termination: 6.2 to 6.3 - Moisture:
- Water content at initiation: 32.3% to 33.4% (56.7% to 58.6% of the maximum water holding capacity)
Water content at termination: 32.0% to 34.7% (56.2% to 60.9% of the maximum water holding capacity) - Nominal and measured concentrations:
- Nominal: Control, 0.63, 1.25, 2.5, 5.0 and 10 mg/kg soil
- Reference substance (positive control):
- yes
- Remarks:
- Luxan Carbendazim 500 FC (Carbendazim, 500 g/L nominal)
Results and discussion
Effect concentrations
- Key result
- Duration:
- 56 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: highest concentration tested
- Results with reference substance (positive control):
- There were statistically significant effects on reproduction at a concentration of 1.30 mg carbendazim/kg soil and higher; the EC50 for reproduction was calculated as 1.32 mg carbendazim/kg soil. The results indicating that the sensitivity of the worms was consistent with the level proposed by the OECD 222 guideline (significant effects between 1 and 5 mg carbendazim/kg soil).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 56-day LC50 (Earthworm): > 10 mg/kg soil
- Executive summary:
A 56-day test in treated artificial soil according to OECD 222 was conducted; different concentrations of the test item were incorporated into the soil; 6 treatment groups (5 test item concentrations, control); 4 replicates for the test item treatments, 8 replicates for the control, 10 worms each.
Assessment of adult worm mortality, behavioural effects and biomass development was carried out after 28 days exposure of adult worms in treated artificial soil. Reproduction rate (number of offspring) was assessed after additional 28 days (assessed 56 days after application).
All study validity criteria were met. A slight mortality of up to 5% was found in the test item treatment groups which was not statistically significantly different compared to the control, where 2.5% of the worms died (Fisher´s Exact Test, α = 0.05).
Body weights of the earthworms exposed to the test substance were not statistically significantly different compared to the control up to and including the highest test concentration of 10 mg test item/kg soil, Dunnett´s t-test, α = 0.05).
Statistically significant effects on reproduction were observed at the concentration of 5.0 mg test item/kg only. At all other concentrations tested the reproduction rates were not statistically significantly reduced compared to the control group (Bonferroni-Welch t-test, α =0.05). No behavioural abnormalities were observed in any of the treatment groups and the feeding activity in all the treated groups was comparable to the control.
In an earthworm reproduction and growth study with the test substance, the LC50 was estimated to be greater than 10 mg test item/kg soil. The No Observed Effect Concentration (NOEC) for mortality and weight changes was determined to be the concentration of 10 mg test item/kg soil. The No Observed Effect Concentration (NOEC) for reproduction was determined to be the concentration of 2.5 mg test item/kg soil.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.