Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-942-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- TEST ORGANISM:
- mixed population of aquatic microorganisms, origin of an aeration tank of a domestic sewage treatment plant (Municipal STP Cologne-Stammheim, Germany);
- pre-treatment: aeration
- daily feed: with synthetic medium
- pH-value: 7.5 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- No data
- Test temperature:
- 21.9 °C (concerning the highest concentration of 320 mg/L)
- pH:
- 7.7 (concerning the highest concentration of 320 mg/L)
- Dissolved oxygen:
- oxygen (mg/L): O2-start 5.7; O2-end 5.5 (concerning the highest concentration of 320 mg/L)
- Salinity:
- n.a.
- Conductivity:
- No data
- Nominal and measured concentrations:
- - 32, 56, 100, 180, 320 mg/L (nominal)
- 10000 mg/L (Test item concentration in physico-chemical oxygen consumption control) - Details on test conditions:
- PRETREATMENT:
- test item has been added to 130 mL deionized water, stirred for 2 hours (equilibration phase)
TEST CONDITIONS:
- type of application: direct weighing
- test concentration of the activated sludge: 840 mg/L suspended solids
- temperature: 20 +/- 2 °C
- stirring period before start of incubation time: 17 h
- test concentration: 5 (32, 56, 100, 180, 320 mg/L)
- test item in physico-chemical oxygen consumption control: 1000 mg/L
- test parameter: respiration rate
- aeration: permanent
- reference substance: 3,5-Dichlorphenol (2.5, 5, 10, 20, 40 mg/L) - Reference substance (positive control):
- yes
- Remarks:
- 3.5 Dichlorophenol (Aldrich)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
Respiration inhibition (Test item):
- 32 mg/L: 33.3 %
- 56 mg/L: 41.7 %
- 100 mg/L: 66.7 %
- 180 mg/L: 76.7 %
- 320 mg/L: 83.3 % - Results with reference substance (positive control):
- - 2.5 mg/L: 8.3 % respiration inhibition
- 5 mg/L: 11.1 % respiration inhibition
- 10 mg/L: 50.0 % respiration inhibition
- 20 mg/L: 88.9 % respiration inhibition
- 40 mg/L: 94.4 % respiration inhibition - Validity criteria fulfilled:
- yes
- Remarks:
- (respiratory rates of the 2 controls differ less than 15 % from each other; the EC50 of the reference compound 3,5-dichlorophenol is in the range 5–30 mg/L)
- Conclusions:
- The substance showed 83.3 % respiration inhibition of activated sludge at a test item concentration of 320 mg/L. A 3 h-EC50 of 64 mg/L was determined. Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
- Executive summary:
In order to test the toxicity to microorganism of the substance, activated sludge from predominantly domestic sewage plant was exposed to the test solution of five nominal concentrations of the substance (32, 56, 100, 180, 320 mg/L), blank control solution and solution with positive reference substance for a period of 3 hours. Dissolved oxygen was recorded and respiration rates were claculated. 83.3 % respiration inhibition was observed at the highest concentration of test substance. The physico-chemical oxygen consumption has been determined at 10000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations. The substance showed 83.3 % respiration inhibition of activated sludge at a test item concentration of 320 mg/L. A 3 h-EC50 of 64 mg/L was determined. Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microoragnisms.
Reference
The physico-chemical oxygen consumption has been determined at 10000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
Description of key information
The substance showed 83.3 % respiration inhibition of activated sludge at a test item concentration of 320 mg/L. A 3 h-EC50 of 64 mg/L was determined. Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods (Neuhahn, 2006).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 64 mg/L
Additional information
The physico-chemical oxygen consumption has been determined at 10000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.