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EC number: 700-579-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- no
Test material
- Reference substance name:
- tetrakis(4-{[4-(diethylamino)phenyl][4-(ethylamino)naphthalen-1-yl]methylidene}-N,N-diethylcyclohexa-2,5-dien-1-iminium) 2-(dodecan-5-yl)-4-(4-sulfonatophenoxy)benzene-1-sulfonate 2-(dodecan-5-yl)-4-[3-(dodecan-5-yl)-4-sulfonatophenoxy]benzene-1-sulfonate
- EC Number:
- 700-579-6
- Molecular formula:
- C90H114O7S2N6 (MW = 1454) and C102H138O7S2N6 (MW = 1622)
- IUPAC Name:
- tetrakis(4-{[4-(diethylamino)phenyl][4-(ethylamino)naphthalen-1-yl]methylidene}-N,N-diethylcyclohexa-2,5-dien-1-iminium) 2-(dodecan-5-yl)-4-(4-sulfonatophenoxy)benzene-1-sulfonate 2-(dodecan-5-yl)-4-[3-(dodecan-5-yl)-4-sulfonatophenoxy]benzene-1-sulfonate
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- - Batch 310551
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 14 ml of test item diluted in 1 L liters of ultra-pure water (stock solution).
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not mentionned
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia Magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- Room temperature
- pH:
- 7.7 à 7.9 from T0 to T48h
- Dissolved oxygen:
- 8.2 mg/L to 8.3mg/L at T0
7.2mg/L to 8.0 mg/L at T48H - Nominal and measured concentrations:
- Nominal concentration:
0.18 - 0.11 - 0.063 - 0.036 - 0.021 - 0.011 - 0.007 - 0.0042 - 0.0025 - 0.0014 mg/L - Details on test conditions:
- TEST SYSTEM
- Type of flow-through : static
- No. of organisms per vessel: 20
- No. of vessels per concentration : 4
- No. of vessels per control (replicates): 4
TEST CONCENTRATIONS
- Range finding study: 12.6 - 4.9 - 1.4 - 0.5 - 0.14 - 0.05 - 0.014 - 0.0049 mg/L
- Test concentrations: 0.18 - 0.11 - 0.063 - 0.036 - 0.021 - 0.011 - 0.007 - 0.0042 - 0.0025 - 0.0014 mg/L
- Results used to determine the conditions for the definitive study:
12.6 mg/L: 100% immobilisation
4.9 mg/L = 100% immobilisation
1.4 mg/L = 100 % immobilisation
0.5 mg/L = 100 % immobilisation
0.14 mg/L = 100 % immobilisation
0.05 mg/L = 90% immobilisation
0.014 mg/L = 20% immobilisation
0.0049 mg/L = 0% immobilisation
- Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.012 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI : 0.0095-0.015
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.006 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 0.0047-0.0069
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.003 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50 (24h) = 1.0 mg/L - Reported statistics and error estimates:
- EC 50 was calculated by using Log-Probit statistical model (software Toxcalc)
NOEC was calculated by using the Bonferroni t-test (software Toxcalc).
Any other information on results incl. tables
ASSAY:
T = 24 hours |
|||||||||||
Concen- tration (mg/L) |
0.18 |
0.11 |
0.063 |
0.036 |
0.021 |
0.011 |
0.007 |
0.0042 |
0.0025 |
0.0014 |
Control |
I |
0 |
0 |
0 |
0 |
2 |
3 |
5 |
3 |
5 |
5 |
5 |
II |
0 |
0 |
0 |
1 |
1 |
3 |
2 |
4 |
5 |
5 |
5 |
III |
0 |
0 |
1 |
1 |
2 |
4 |
3 |
5 |
5 |
5 |
5 |
IV |
0 |
0 |
0 |
0 |
1 |
2 |
3 |
4 |
5 |
5 |
5 |
Total moving |
0 |
0 |
1 |
2 |
6 |
12 |
13 |
16 |
20 |
20 |
20 |
Immobilization rate % |
100% |
100% |
95% |
90% |
70% |
40% |
35% |
20% |
0% |
0% |
0% |
T = 48 hours |
|||||||||||
Concen- tration (mg/L) |
0.18 |
0.11 |
0.063 |
0.036 |
0.021 |
0.011 |
0.007 |
0.0042 |
0.0025 |
0.0014 |
Control |
I |
0 |
0 |
0 |
0 |
0 |
1 |
3 |
2 |
4 |
5 |
5 |
II |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
4 |
5 |
5 |
5 |
III |
0 |
0 |
0 |
1 |
0 |
0 |
2 |
4 |
4 |
5 |
5 |
IV |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
4 |
5 |
5 |
5 |
Total moving |
0 |
0 |
0 |
0 |
1 |
2 |
7 |
14 |
18 |
20 |
20 |
Immobilization rate % |
100% |
100% |
100% |
100% |
95% |
90% |
65% |
20% |
0% |
0% |
0% |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions:
24-h EC50=0.012 mg/L (IC 95% : 0.0095; 0.015)
48-h EC50=0.0057 mg/L. (IC 95% : 0.0047; 0.0069)
48-h NOEC= 0.0025 mg/mL - Executive summary:
The short-term toxicity (48 hours) on invertebrate (Daphnia magna) of the test item Sepisol Fast Blue 85219 was assessed by determining the nominal effective concentration (EC 50%) at which 50 % of the test animals are immobilized. The method followed was the standard NF EN ISO 6341 under static condition.
EC50 (48h) values and associated confidence limits were calculated using a Log-Probit statistical model (software Toxcalc) and NOEC was worked out by using the Bonferroni t-test (software Toxcalc).
Test System: A stock solution (14 mg/mL) of the test substance was prepared in ultra-pur water.
Immobilisations and viable abnormalities were recorded at 24 and 48 hours as well as pH and dissolved oxygen.
Validity criteria were fulfilled and study was considered as valid..
A preliminary assay was performed at the following concentration: 12.6 - 4.9 - 1.4 - 0.5 - 0.014 - 0.0049 mg/L
Test Levels: Test concentrations included control, 0.18 - 0.11 - 0.063 - 0.036 - 0.021 - 0.011 - 0.007 - 0.0042 - 0.0025 - 0.0014 mg/L. Twenty daphnia were used per test group. 3 replicates were performed.
Conclusion:
24-h EC50=0.012 mg/L (IC 95%: 0.0095-0.015)
48-h EC50=0.0057 mg/L (IC 95%: 0.0047-0.0069)
48-h NOEC= 0.0025 mg/mL
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