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EC number: 916-222-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29.10.1998-26.11.1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 98/Ba736/1 11 09 98
- Expiration date of the lot/batch: not specified
- Purity test date: not specified
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room tempearture, away from moisture
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: no solubility
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not reported
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: not specified
- Preliminary purification step (if any): not needed
- Final dilution of a dissolved solid, stock liquid or gel: not applicable
- Final preparation of a solid: not applicable - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: Chantilly 60, France, receiving predominantly domestic sewage.
- Storage conditions: used immidiately
- Storage length: used immidiately
- Pretreatment: The freshly obtained sludge was used immediately. The concentration of suspended micro-organisms was determined to be 1.10^5 /mL in the concentrated sludge. Before use, the sludge was allowed to settle and the supernatant liquid was used as inoculum at the amount of 30 mL/L of mineral medium.
- Initial cell/biomass concentration: 1.10^5 micro-organisms/mL
- Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- >= 10 - <= 20 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: see table 1 below
- Additional substrate: not specified
- Test temperature: ca. 20C
- pH: 7.55
- pH adjusted: not specified
- CEC (meq/100 g): not specified
- Aeration of dilution water: CO2 free compressed air
- Suspended solids concentration: not specified
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Details of trap for CO2 and volatile organics if used:
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 27
- St. dev.:
- 3
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the conditions and duration of the test the product Stepantex X 1240 shows a low degradation.
- Executive summary:
Objective of the study
Determine under defined conditions, the biodegradability of an organic product in a mineral liquid medium inoculates a microbial flora of wastewater.
The biodegradability is experimee in% of theoretical CO2 produced.
Method of the study:
OECD Guideline for the Testing of Chemicals: Ready Biodegradability - Method 301B (17 July 1992): CO2 evolution test (modified Sturm).
Substances tested:
Agent 2076 -43D
Agent X 2197 -7 -7
Stepantex X 1040/2
Stepantex X 1040/1
Stepantex X 1240
Concentrations used:
10 and 20 mg / l organic carbon (initial, nominal)
Reference substance:
Aniline; 20 mg/l organic carbon
Inoculum:
Prepared from a sample of activated sludge from an urban wastewater treatment plant.
Duration of the study:
28 days
Results of the tests
The biodegradability of the five substances is evaluated at the end of the test (28 days):
As a percentage of theoretical CO2:
Concentration Agent 2076-43D Agent X 2197-7-7 Stepantex X 1040/2 Stepantex X 1040/1 Stepantex X 1240 Aniline
(ref.)10 mg/L 50% 37% 70% 74% 29% - 20mg/L 53% 43% 69% 77% 24% 93% Average 51% 40% 70% 75% 27% - As percentage of TOC removal:
Concentration Agent 2076-43D Agent X 2197-7-7 Stepantex X 1040/2 Stepantex X 1040/1 Stepantex X 1240 Aniline
(ref.)10 mg/L 77% 60% 84% 80% 49% - 20mg/L 72% 66% 92% 90% 44% 98% Average 75% 63% 88% 85% 47% - Conclusion:
Under the conditions and the test period, only the two ST and SP substances meet the criteria for ready biodegradability.
Reference
Table 1: CO degradation
Day | CO2 Degradation | Cumulative CO2 production | Theoretical CO2 % | ||||||||||
Blanks | Assay | Assay | Assay | ||||||||||
FB1+FB2 | FB3 | FB average | FB9 | FB10 | FB total | FB9 | FB10 | FB total | FB9 | FB10 | FB average | FB total | |
1 | 2.09 | 5.28 | 3.66 | 1.65 | 1.98 | 3.74 | 0 | 0 | 0.05 | 0 | 0 | 0 | 0.02 |
4 | 5.83 | 11.44 | 8.83 | 7.37 | 8.89 | 43.67 | 0 | 0.05 | 35.05 | 0 | 0.02 | 0.01 | 15.92 |
5 | 13.66 | 15.62 | 14.74 | 17.93 | 22.66 | 81.52 | 3.19 | 7.92 | 70.78 | 2.9 | 3.6 | 3.25 | 34.9 |
6 | 14.74 | 15.95 | 15.34 | 19.47 | 27.5 | 133.96 | 4.12 | 12.15 | 118.83 | 3.75 | 5.52 | 4.84 | 53.92 |
7 | 16.39 | 16.81 | 16.61 | 21.56 | 31.46 | 153.45 | 4.95 | 14.85 | 136.64 | 4.5 | 6.75 | 5.82 | 62.2 |
8 | 17.93 | 17.49 | 17.71 | 24.2 | 35.66 | 167.88 | 6.49 | 18 | 150.15 | 5.9 | 8.25 | 7.07 | 68.25 |
11 | 18.15 | 19.03 | 18.59 | 28.6 | 44 | 186.78 | 10.01 | 15 | 168.18 | 9.1 | 11.56 | 10.32 | 76.45 |
12 | 18.15 | 19.8 | 18.97 | 30.36 | 42.74 | 193.34 | 11.38 | 25.41 | 174.4 | 10.35 | 13.07 | 11.71 | 79.27 |
14 | 18.37 | 20.02 | 19.19 | 32.56 | 52.14 | 200.75 | 13.38 | 28.78 | 181.55 | 12.15 | 14.97 | 13.56 | 82.52 |
15 | 18.58 | 20.13 | 19.36 | 34.32 | 55.33 | 205.15 | 14.96 | 32.94 | 185.79 | 13.6 | 16.35 | 14.97 | 84.75 |
18 | 18.58 | 20.57 | 19.56 | 37.16 | 59.07 | 214.83 | 17.6 | 35.97 | 195.25 | 16 | 18.2 | 17.1 | 88.75 |
20 | 19.25 | 21.01 | 20.13 | 39.27 | 63.14 | 220.33 | 19.14 | 40.04 | 200.2 | 17.4 | 19.55 | 18.47 | 91 |
22 | 20.66 | 21.01 | 20.84 | 42.48 | 65.47 | 222.42 | 21.61 | 43.01 | 201.52 | 18.85 | 20.32 | 20.01 | 91.42 |
25 | 21.56 | 21.01 | 21.28 | 45.87 | 66.42 | 222.42 | 24.58 | 44.82 | 201.13 | 22.35 | 21.42 | 21.89 | 91.42 |
27 | 22.66 | 21.01 | 21.83 | 47.65 | 66.63 | 223.96 | 26.01 | 47.13 | 202.12 | 23.85 | 21.72 | 22.69 | 91.87 |
28 | 22.99 | 21.01 | 22 | 54.34 | 74.25 | 225.3 | 32.34 | 47.79 | 203.5 | 29.4 | 23.75 | 26.57 | 92.5 |
Table 2: Theoretical CO2 %
Stepantex X 1240 | ||
Flask | FB 9 10 mg/L |
FB 10 20 mg/L |
Days | - | - |
4 | 0.0% | 0.0% |
7 | 4.5% | 6.7% |
14 | 12.1% | 15.0% |
22 | 19.6% | 20.4% |
28 | 29.4% | 23.7% |
Description of key information
Under the conditions and duration of the test the product Stepantex X 1240 shows a low degradation.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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