Reaction mass of 2,2'-(vinylenedi-p-phenylene)bis[5-methylbenzoxazole] and 2,2'-(vinylenedi-p- phenylene)bisbenzoxazole and 2-[4-[2-[4-(benzoxazol-2- yl)phenyl]vinyl]phenyl]-5-methylbenzoxazole

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August from 03 to 10, 1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Principles of method if other than guideline:

The acute lethal toxicity of the test substance to Leuciscus idus f. melanotus was investigated under static exposure conditions over a period of 96 hours. 10 fish were exposed to one of the following nominal dose levels: 0, 1, 10, 100 and 500 mg/l .No chemical analysis of the test substance concentration was conducted.

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: goldorfe.
- Source: Paul Eggers, 2354 Hohenwestedt.
- Length at study initiation: 3.5 - 4.4 cm (mean = 3.9 cm; s = 0.2 g; n = 50).
- Weight at study initiation: 0.7 - 1.2 g (mean = 0.88 g; s = 0.13 g; n = 50).

ACCLIMATION
- Acclimation period: the fish were adapted to the experimental conditions at least 14 days prior to the experiment.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

20 ± 0.5 °C

Nominal and measured concentrations:

0, 1, 10, 100 and 500 mg/l nominal.

Details on test conditions:

TEST SYSTEM
- Test vessel: full-glass aquariums, 40 × 25 × 30 cm.
- Volume: 20 litres.
- No. of organisms per vessel: 10.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chlorinated tap water, treated with activated carbon filter.
- Total hardness: 17.8°dH; carbonate hardness 12°dH.
- pH: 7.8

OTHER TEST CONDITIONS
- Photoperiod: 12 hours of light and 12 of darkness.
- Light intensity: 700 Lux.

EFFECT PARAMETERS MEASURED
During the whole experiment, the fish were regularly observed and the behavior recorded.
The surviving fish was sacrificed after the end of the experiment and macroscopically examined.
The oxygen content and the pH values were checked at the beginning of the experiment and after 24-hour intervals.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Over the whole exposure period, no fish died in none of the concentration group.
The behavior was unaffected in all experimental groups and did not differ from that of the control fish. The section of the fish slaughtered at the end of the experiment revealed yellow-discolored intestinal contents in all experimental groups.

Conclusions:

LC50 (96h) > 500 mg/l (nominal)

Executive summary:

The acute lethal toxicity of test substance to Leuciscus idus f. melanotus was investigated under static exposure conditions over a period of 96 hours. 10 fish were exposed to each one of the follwoing nominal dose levels: 0, 1, 10, 100 and 500 mg/l. No chemical analysis of the test substance concentrations was conducted.

Over the whole exposure period, no fish died in none of the concentration groups. The behavior was unaffected in all experimental groups and did not differ from that of the control fish. The section of the fish slaughtered at the end of the experiment revealed yellow-discolored intestinal contents in all experimental groups.

Conclusion

LC50 (96h) > 500 mg/l (nominal)

Description of key information

Not harmful/toxic to fish, after short-term exposure.

Additional information

The acute lethal toxicity of the Fluorescent Brightener 219 was assessed in Leuciscus idus f. melanotus, under static exposure conditions. Fish were exposed to the following nominal dose levels: 0, 1, 10, 100 and 500 mg/l; no chemical analysis of the test substance concentrations was conducted. Over the whole exposure period of 96 hours, no fish died in none of the concentration groups. The behavior was unaffected in all experimental groups and did not differ from that of the control fish. The section of the fish slaughtered at the end of the experiment revealed yellow-discolored intestinal contents in all experimental groups.

In order to support the study outcomes, available data on one of the substance constituents (i.e. constituent 2) has also been taken into consideration. The acute lethal toxicity of the substance was investigated on guppy, under static exposure conditions over a period of 96 h. The only applied nominal test concentration was 100 mg/l. Due to the limited water solubility of the test material the respective amount was added as an acetonic solution to the empty test vessel. After complete evaporation of the solvent, the test water was added. No chemical analysis of the test substance concentration was conducted. At the nominal concentration of 100 mg/l one of a total of seven fish died after 96h of exposure. Other toxic effects than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed.

The no-observed-effect concentration (NOEC) was higher than 100 mg/l since the one fish that died is considered not to represent a significant effect as defined by the validity criteria of the test.

In both the cases, no acute adverse effects were recorded at the highest tested dosages; thus, the substance resulted to be not harmful/toxic to fish, after short-term exposure.