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EC number: 604-102-1 | CAS number: 13893-53-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-amino-2,3-dimethylbutanenitrile
- Cas Number:
- 13893-53-3
- Molecular formula:
- C6H12N2
- IUPAC Name:
- 2-amino-2,3-dimethylbutanenitrile
- Details on test material:
- - Name of test material (as cited in study report): Butanenitrile-2-amino-2.3-dimethyl
- Purity: 93.7 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 200.0 mg of the test substance was added to about 800 mL of demineralized water and stirred for about 2 hours at room temperature until the test substance was completely dissolved. After that the pH-value was measured (measured value = 9.1) and adjusted to 7.3 with 1 M sulphuric acid solution. The stock solution was made up to 1L with demineralized water. The stock solution appeared clear and no undissolved test substance was vissible.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant of Mannheim, Germany was collected on 23 Mar 2010 from the aeration tank of the plant. The activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed with tap water and the suspension was adjusted to 7.5 g/L dry matter.
An aliquot of this suspension was added to the test vessels to obtain a sludge concentration of 1.5 g/L dry substance.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
Test conditions
- Test temperature:
- 20 +/- 2 °C
- pH:
- The pH-values were measured in all test vessels. An adjustment was not necessary.
- Dissolved oxygen:
- Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement: > 6.5 mg/L - Nominal and measured concentrations:
- Test concentrations (nominal): 62.5, 21.0, 6.9, 2.3, 0.8 mg/L and blank controls.
- Details on test conditions:
- - Test vessels: Erlenmeyer-vessel (nominal volume 250 mL)
- Test volume: 250 mL
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
- Duration of the measurement of oxygen consumption: 8-10 min
- Number of test assays per concentration: 1 - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 min
- Dose descriptor:
- EC10
- Effect conc.:
- 1.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- 7.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- EC20 (180min) = 2.1 mg/L
EC80 (180min) = 24 mg/L
In a former respiration inhibition test the test substance had an inhibition rate of 69% at a test concentration of 62.5 mg/L. Thus the mentioned test concentrations were selected.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.